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Transdifferentiation of Myoblasts Into Adipocytes by All-Trans-Retinoic Acid in Avian

Increased adipogenesis in muscle tissues is related to metabolic syndromes and muscle weakness in humans and improvement of meat quality in animal production. With growing evidence for pro-adipogenic functions of all-trans-retinoic acid (atRA), the current study investigated whether atRA can transdi...

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Autores principales: Kim, Dong-Hwan, Lee, Joonbum, Suh, Yeunsu, Ko, Jae-Kyun, Lee, Kichoon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9019681/
https://www.ncbi.nlm.nih.gov/pubmed/35465310
http://dx.doi.org/10.3389/fcell.2022.856881
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author Kim, Dong-Hwan
Lee, Joonbum
Suh, Yeunsu
Ko, Jae-Kyun
Lee, Kichoon
author_facet Kim, Dong-Hwan
Lee, Joonbum
Suh, Yeunsu
Ko, Jae-Kyun
Lee, Kichoon
author_sort Kim, Dong-Hwan
collection PubMed
description Increased adipogenesis in muscle tissues is related to metabolic syndromes and muscle weakness in humans and improvement of meat quality in animal production. With growing evidence for pro-adipogenic functions of all-trans-retinoic acid (atRA), the current study investigated whether atRA can transdifferentiate myoblasts into adipocytes using a quail myogenic cell line (QM7) and avian primary myoblasts. atRA increased cytoplasmic lipid droplet accumulation and mRNA expression for adipogenic genes in these cells. An acute induction of Pparγ expression by atRA under cycloheximide treatment indicated a direct regulation of Pparγ by atRA. In addition, the induction of Pparγ expression was mediated by retinoic acid receptors . At high levels of Pparγ by atRA, BADGE, an antagonist of Pparγ, inhibited, and rosiglitazone, an agonist of Pparγ, further enhanced atRA-induced transdifferentiation. However, at very low levels of Pparγ in the absence of atRA treatment, rosiglitazone could not induce transdifferentiation of avian myoblasts. These data suggest that the induction of Pparγ expression by atRA is an essential molecular event in myoblasts for atRA-induced transdifferentiation into adipocytes. Based on our findings, atRA can be a new transdifferentiation factor of myoblasts to adipocytes, providing a potential nutrient to enhance marbling in poultry.
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spelling pubmed-90196812022-04-21 Transdifferentiation of Myoblasts Into Adipocytes by All-Trans-Retinoic Acid in Avian Kim, Dong-Hwan Lee, Joonbum Suh, Yeunsu Ko, Jae-Kyun Lee, Kichoon Front Cell Dev Biol Cell and Developmental Biology Increased adipogenesis in muscle tissues is related to metabolic syndromes and muscle weakness in humans and improvement of meat quality in animal production. With growing evidence for pro-adipogenic functions of all-trans-retinoic acid (atRA), the current study investigated whether atRA can transdifferentiate myoblasts into adipocytes using a quail myogenic cell line (QM7) and avian primary myoblasts. atRA increased cytoplasmic lipid droplet accumulation and mRNA expression for adipogenic genes in these cells. An acute induction of Pparγ expression by atRA under cycloheximide treatment indicated a direct regulation of Pparγ by atRA. In addition, the induction of Pparγ expression was mediated by retinoic acid receptors . At high levels of Pparγ by atRA, BADGE, an antagonist of Pparγ, inhibited, and rosiglitazone, an agonist of Pparγ, further enhanced atRA-induced transdifferentiation. However, at very low levels of Pparγ in the absence of atRA treatment, rosiglitazone could not induce transdifferentiation of avian myoblasts. These data suggest that the induction of Pparγ expression by atRA is an essential molecular event in myoblasts for atRA-induced transdifferentiation into adipocytes. Based on our findings, atRA can be a new transdifferentiation factor of myoblasts to adipocytes, providing a potential nutrient to enhance marbling in poultry. Frontiers Media S.A. 2022-04-06 /pmc/articles/PMC9019681/ /pubmed/35465310 http://dx.doi.org/10.3389/fcell.2022.856881 Text en Copyright © 2022 Kim, Lee, Suh, Ko and Lee. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Kim, Dong-Hwan
Lee, Joonbum
Suh, Yeunsu
Ko, Jae-Kyun
Lee, Kichoon
Transdifferentiation of Myoblasts Into Adipocytes by All-Trans-Retinoic Acid in Avian
title Transdifferentiation of Myoblasts Into Adipocytes by All-Trans-Retinoic Acid in Avian
title_full Transdifferentiation of Myoblasts Into Adipocytes by All-Trans-Retinoic Acid in Avian
title_fullStr Transdifferentiation of Myoblasts Into Adipocytes by All-Trans-Retinoic Acid in Avian
title_full_unstemmed Transdifferentiation of Myoblasts Into Adipocytes by All-Trans-Retinoic Acid in Avian
title_short Transdifferentiation of Myoblasts Into Adipocytes by All-Trans-Retinoic Acid in Avian
title_sort transdifferentiation of myoblasts into adipocytes by all-trans-retinoic acid in avian
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9019681/
https://www.ncbi.nlm.nih.gov/pubmed/35465310
http://dx.doi.org/10.3389/fcell.2022.856881
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