Protocol for electroretinogram recording of the Drosophila compound eye

Drosophila phototransduction is a well-established model for characterizing biological processes, such as calcium signaling. Here, we present a protocol for electroretinogram (ERG) recording, an in-vivo physiological assay extensively used in Drosophila phototransduction research to measure light-ev...

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Autores principales: Wu, Jinglin, Tian, Yao, Dong, Wei, Han, Junhai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9019699/
https://www.ncbi.nlm.nih.gov/pubmed/35463470
http://dx.doi.org/10.1016/j.xpro.2022.101286
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author Wu, Jinglin
Tian, Yao
Dong, Wei
Han, Junhai
author_facet Wu, Jinglin
Tian, Yao
Dong, Wei
Han, Junhai
author_sort Wu, Jinglin
collection PubMed
description Drosophila phototransduction is a well-established model for characterizing biological processes, such as calcium signaling. Here, we present a protocol for electroretinogram (ERG) recording, an in-vivo physiological assay extensively used in Drosophila phototransduction research to measure light-evoked field potential responses of photoreceptors and neurons in the lamina neuropil. We describe fly preparation and electrode placement, followed by pretest setup, ERG recording, and data analysis. This protocol enables assessment of photoreceptor performance and visual synaptic transmission between photoreceptors and downstream lamina neurons. For complete details on the use and execution of this protocol, please refer to Han et al. (2006), Hu et al. (2012), and Wu et al. (2021).
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spelling pubmed-90196992022-04-21 Protocol for electroretinogram recording of the Drosophila compound eye Wu, Jinglin Tian, Yao Dong, Wei Han, Junhai STAR Protoc Protocol Drosophila phototransduction is a well-established model for characterizing biological processes, such as calcium signaling. Here, we present a protocol for electroretinogram (ERG) recording, an in-vivo physiological assay extensively used in Drosophila phototransduction research to measure light-evoked field potential responses of photoreceptors and neurons in the lamina neuropil. We describe fly preparation and electrode placement, followed by pretest setup, ERG recording, and data analysis. This protocol enables assessment of photoreceptor performance and visual synaptic transmission between photoreceptors and downstream lamina neurons. For complete details on the use and execution of this protocol, please refer to Han et al. (2006), Hu et al. (2012), and Wu et al. (2021). Elsevier 2022-04-11 /pmc/articles/PMC9019699/ /pubmed/35463470 http://dx.doi.org/10.1016/j.xpro.2022.101286 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Wu, Jinglin
Tian, Yao
Dong, Wei
Han, Junhai
Protocol for electroretinogram recording of the Drosophila compound eye
title Protocol for electroretinogram recording of the Drosophila compound eye
title_full Protocol for electroretinogram recording of the Drosophila compound eye
title_fullStr Protocol for electroretinogram recording of the Drosophila compound eye
title_full_unstemmed Protocol for electroretinogram recording of the Drosophila compound eye
title_short Protocol for electroretinogram recording of the Drosophila compound eye
title_sort protocol for electroretinogram recording of the drosophila compound eye
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9019699/
https://www.ncbi.nlm.nih.gov/pubmed/35463470
http://dx.doi.org/10.1016/j.xpro.2022.101286
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