Protocol for electroretinogram recording of the Drosophila compound eye
Drosophila phototransduction is a well-established model for characterizing biological processes, such as calcium signaling. Here, we present a protocol for electroretinogram (ERG) recording, an in-vivo physiological assay extensively used in Drosophila phototransduction research to measure light-ev...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9019699/ https://www.ncbi.nlm.nih.gov/pubmed/35463470 http://dx.doi.org/10.1016/j.xpro.2022.101286 |
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author | Wu, Jinglin Tian, Yao Dong, Wei Han, Junhai |
author_facet | Wu, Jinglin Tian, Yao Dong, Wei Han, Junhai |
author_sort | Wu, Jinglin |
collection | PubMed |
description | Drosophila phototransduction is a well-established model for characterizing biological processes, such as calcium signaling. Here, we present a protocol for electroretinogram (ERG) recording, an in-vivo physiological assay extensively used in Drosophila phototransduction research to measure light-evoked field potential responses of photoreceptors and neurons in the lamina neuropil. We describe fly preparation and electrode placement, followed by pretest setup, ERG recording, and data analysis. This protocol enables assessment of photoreceptor performance and visual synaptic transmission between photoreceptors and downstream lamina neurons. For complete details on the use and execution of this protocol, please refer to Han et al. (2006), Hu et al. (2012), and Wu et al. (2021). |
format | Online Article Text |
id | pubmed-9019699 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-90196992022-04-21 Protocol for electroretinogram recording of the Drosophila compound eye Wu, Jinglin Tian, Yao Dong, Wei Han, Junhai STAR Protoc Protocol Drosophila phototransduction is a well-established model for characterizing biological processes, such as calcium signaling. Here, we present a protocol for electroretinogram (ERG) recording, an in-vivo physiological assay extensively used in Drosophila phototransduction research to measure light-evoked field potential responses of photoreceptors and neurons in the lamina neuropil. We describe fly preparation and electrode placement, followed by pretest setup, ERG recording, and data analysis. This protocol enables assessment of photoreceptor performance and visual synaptic transmission between photoreceptors and downstream lamina neurons. For complete details on the use and execution of this protocol, please refer to Han et al. (2006), Hu et al. (2012), and Wu et al. (2021). Elsevier 2022-04-11 /pmc/articles/PMC9019699/ /pubmed/35463470 http://dx.doi.org/10.1016/j.xpro.2022.101286 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Protocol Wu, Jinglin Tian, Yao Dong, Wei Han, Junhai Protocol for electroretinogram recording of the Drosophila compound eye |
title | Protocol for electroretinogram recording of the Drosophila compound eye |
title_full | Protocol for electroretinogram recording of the Drosophila compound eye |
title_fullStr | Protocol for electroretinogram recording of the Drosophila compound eye |
title_full_unstemmed | Protocol for electroretinogram recording of the Drosophila compound eye |
title_short | Protocol for electroretinogram recording of the Drosophila compound eye |
title_sort | protocol for electroretinogram recording of the drosophila compound eye |
topic | Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9019699/ https://www.ncbi.nlm.nih.gov/pubmed/35463470 http://dx.doi.org/10.1016/j.xpro.2022.101286 |
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