Isolation and culture of pure adult mouse microglia and astrocytes for in vitro characterization and analyses

Microglia and astrocytes are implicated in aging and age-related diseases. Here, we present a protocol to isolate and culture these glia cells from the murine brain. The protocol consists of two parts: magnetic sorting of adult microglia and mechanical/magnetic sorting of adult microglia and astrocy...

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Detalles Bibliográficos
Autores principales: Milner, Mark T., Lawrence, Grace MEP., Holley, Caroline L., Bodea, Liviu-Gabriel, Götz, Jürgen, Burgener, Sabrina S., Schroder, Kate
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9019703/
https://www.ncbi.nlm.nih.gov/pubmed/35463473
http://dx.doi.org/10.1016/j.xpro.2022.101295
Descripción
Sumario:Microglia and astrocytes are implicated in aging and age-related diseases. Here, we present a protocol to isolate and culture these glia cells from the murine brain. The protocol consists of two parts: magnetic sorting of adult microglia and mechanical/magnetic sorting of adult microglia and astrocytes. We then describe the characterization of these glial cells by flow cytometry and immunohistochemistry. Microglia isolated from aged mice maintain age-related phenotype during culture. These purified glia cells can be applied in ex vivo studies.

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