Isolation and culture of pure adult mouse microglia and astrocytes for in vitro characterization and analyses

Microglia and astrocytes are implicated in aging and age-related diseases. Here, we present a protocol to isolate and culture these glia cells from the murine brain. The protocol consists of two parts: magnetic sorting of adult microglia and mechanical/magnetic sorting of adult microglia and astrocy...

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Autores principales: Milner, Mark T., Lawrence, Grace MEP., Holley, Caroline L., Bodea, Liviu-Gabriel, Götz, Jürgen, Burgener, Sabrina S., Schroder, Kate
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9019703/
https://www.ncbi.nlm.nih.gov/pubmed/35463473
http://dx.doi.org/10.1016/j.xpro.2022.101295
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author Milner, Mark T.
Lawrence, Grace MEP.
Holley, Caroline L.
Bodea, Liviu-Gabriel
Götz, Jürgen
Burgener, Sabrina S.
Schroder, Kate
author_facet Milner, Mark T.
Lawrence, Grace MEP.
Holley, Caroline L.
Bodea, Liviu-Gabriel
Götz, Jürgen
Burgener, Sabrina S.
Schroder, Kate
author_sort Milner, Mark T.
collection PubMed
description Microglia and astrocytes are implicated in aging and age-related diseases. Here, we present a protocol to isolate and culture these glia cells from the murine brain. The protocol consists of two parts: magnetic sorting of adult microglia and mechanical/magnetic sorting of adult microglia and astrocytes. We then describe the characterization of these glial cells by flow cytometry and immunohistochemistry. Microglia isolated from aged mice maintain age-related phenotype during culture. These purified glia cells can be applied in ex vivo studies.
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spelling pubmed-90197032022-04-21 Isolation and culture of pure adult mouse microglia and astrocytes for in vitro characterization and analyses Milner, Mark T. Lawrence, Grace MEP. Holley, Caroline L. Bodea, Liviu-Gabriel Götz, Jürgen Burgener, Sabrina S. Schroder, Kate STAR Protoc Protocol Microglia and astrocytes are implicated in aging and age-related diseases. Here, we present a protocol to isolate and culture these glia cells from the murine brain. The protocol consists of two parts: magnetic sorting of adult microglia and mechanical/magnetic sorting of adult microglia and astrocytes. We then describe the characterization of these glial cells by flow cytometry and immunohistochemistry. Microglia isolated from aged mice maintain age-related phenotype during culture. These purified glia cells can be applied in ex vivo studies. Elsevier 2022-04-11 /pmc/articles/PMC9019703/ /pubmed/35463473 http://dx.doi.org/10.1016/j.xpro.2022.101295 Text en Crown Copyright © 2022. https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Milner, Mark T.
Lawrence, Grace MEP.
Holley, Caroline L.
Bodea, Liviu-Gabriel
Götz, Jürgen
Burgener, Sabrina S.
Schroder, Kate
Isolation and culture of pure adult mouse microglia and astrocytes for in vitro characterization and analyses
title Isolation and culture of pure adult mouse microglia and astrocytes for in vitro characterization and analyses
title_full Isolation and culture of pure adult mouse microglia and astrocytes for in vitro characterization and analyses
title_fullStr Isolation and culture of pure adult mouse microglia and astrocytes for in vitro characterization and analyses
title_full_unstemmed Isolation and culture of pure adult mouse microglia and astrocytes for in vitro characterization and analyses
title_short Isolation and culture of pure adult mouse microglia and astrocytes for in vitro characterization and analyses
title_sort isolation and culture of pure adult mouse microglia and astrocytes for in vitro characterization and analyses
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9019703/
https://www.ncbi.nlm.nih.gov/pubmed/35463473
http://dx.doi.org/10.1016/j.xpro.2022.101295
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