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Isolation and culture of pure adult mouse microglia and astrocytes for in vitro characterization and analyses
Microglia and astrocytes are implicated in aging and age-related diseases. Here, we present a protocol to isolate and culture these glia cells from the murine brain. The protocol consists of two parts: magnetic sorting of adult microglia and mechanical/magnetic sorting of adult microglia and astrocy...
| Autores principales: | , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9019703/ https://www.ncbi.nlm.nih.gov/pubmed/35463473 http://dx.doi.org/10.1016/j.xpro.2022.101295 |