Generation of precision-cut slice cultures of human placenta

We present a protocol to generate an advanced ex vivo model of human placenta. We use a vibrating tissue slicer to obtain precision-cut slices representative of the entire thickness of human placenta. This approach delivers standardized cultures with a preserved microstructure and cellular compositi...

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Detalles Bibliográficos
Autores principales: Fahmi, Amal, Brügger, Melanie, Zumkehr, Beatrice, Oliveira Esteves, Blandina I., Baud, David, Alves, Marco P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9019714/
https://www.ncbi.nlm.nih.gov/pubmed/35463475
http://dx.doi.org/10.1016/j.xpro.2022.101291
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author Fahmi, Amal
Brügger, Melanie
Zumkehr, Beatrice
Oliveira Esteves, Blandina I.
Baud, David
Alves, Marco P.
author_facet Fahmi, Amal
Brügger, Melanie
Zumkehr, Beatrice
Oliveira Esteves, Blandina I.
Baud, David
Alves, Marco P.
author_sort Fahmi, Amal
collection PubMed
description We present a protocol to generate an advanced ex vivo model of human placenta. We use a vibrating tissue slicer to obtain precision-cut slices representative of the entire thickness of human placenta. This approach delivers standardized cultures with a preserved microstructure and cellular composition comparable to the native tissue. We applied this system to study SARS-CoV-2 infection at the maternal-fetal interface. Moreover, this system can be used to investigate the basic functions of the human placenta in health and disease. For complete details on the use and execution of this protocol, please refer to Fahmi et al. (2021).
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spelling pubmed-90197142022-04-21 Generation of precision-cut slice cultures of human placenta Fahmi, Amal Brügger, Melanie Zumkehr, Beatrice Oliveira Esteves, Blandina I. Baud, David Alves, Marco P. STAR Protoc Protocol We present a protocol to generate an advanced ex vivo model of human placenta. We use a vibrating tissue slicer to obtain precision-cut slices representative of the entire thickness of human placenta. This approach delivers standardized cultures with a preserved microstructure and cellular composition comparable to the native tissue. We applied this system to study SARS-CoV-2 infection at the maternal-fetal interface. Moreover, this system can be used to investigate the basic functions of the human placenta in health and disease. For complete details on the use and execution of this protocol, please refer to Fahmi et al. (2021). Elsevier 2022-04-12 /pmc/articles/PMC9019714/ /pubmed/35463475 http://dx.doi.org/10.1016/j.xpro.2022.101291 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Fahmi, Amal
Brügger, Melanie
Zumkehr, Beatrice
Oliveira Esteves, Blandina I.
Baud, David
Alves, Marco P.
Generation of precision-cut slice cultures of human placenta
title Generation of precision-cut slice cultures of human placenta
title_full Generation of precision-cut slice cultures of human placenta
title_fullStr Generation of precision-cut slice cultures of human placenta
title_full_unstemmed Generation of precision-cut slice cultures of human placenta
title_short Generation of precision-cut slice cultures of human placenta
title_sort generation of precision-cut slice cultures of human placenta
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9019714/
https://www.ncbi.nlm.nih.gov/pubmed/35463475
http://dx.doi.org/10.1016/j.xpro.2022.101291
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