Cloning of DNA oligo pools for in vitro expression

Oligo library pools are powerful tools for systematic investigation of genetic and transcriptomic machinery such as promoter function and gene regulation, non-coding RNAs, or RNA modifications. Here, we provide a detailed protocol for cloning DNA oligo pools made up of tens of thousands of different...

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Detalles Bibliográficos
Autores principales: Uzonyi, Anna, Nir, Ronit, Schwartz, Schraga
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9019715/
https://www.ncbi.nlm.nih.gov/pubmed/35462793
http://dx.doi.org/10.1016/j.xpro.2021.101103
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author Uzonyi, Anna
Nir, Ronit
Schwartz, Schraga
author_facet Uzonyi, Anna
Nir, Ronit
Schwartz, Schraga
author_sort Uzonyi, Anna
collection PubMed
description Oligo library pools are powerful tools for systematic investigation of genetic and transcriptomic machinery such as promoter function and gene regulation, non-coding RNAs, or RNA modifications. Here, we provide a detailed protocol for cloning DNA oligo pools made up of tens of thousands of different constructs, aiming to preserve the complexity of the pools. This system would be suitable for expression in cell lines and can be followed up by next-generation sequencing analysis. For complete details on the use and execution of this profile, please refer to Uzonyi et al. (2021).
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spelling pubmed-90197152022-04-21 Cloning of DNA oligo pools for in vitro expression Uzonyi, Anna Nir, Ronit Schwartz, Schraga STAR Protoc Protocol Oligo library pools are powerful tools for systematic investigation of genetic and transcriptomic machinery such as promoter function and gene regulation, non-coding RNAs, or RNA modifications. Here, we provide a detailed protocol for cloning DNA oligo pools made up of tens of thousands of different constructs, aiming to preserve the complexity of the pools. This system would be suitable for expression in cell lines and can be followed up by next-generation sequencing analysis. For complete details on the use and execution of this profile, please refer to Uzonyi et al. (2021). Elsevier 2022-01-17 /pmc/articles/PMC9019715/ /pubmed/35462793 http://dx.doi.org/10.1016/j.xpro.2021.101103 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Uzonyi, Anna
Nir, Ronit
Schwartz, Schraga
Cloning of DNA oligo pools for in vitro expression
title Cloning of DNA oligo pools for in vitro expression
title_full Cloning of DNA oligo pools for in vitro expression
title_fullStr Cloning of DNA oligo pools for in vitro expression
title_full_unstemmed Cloning of DNA oligo pools for in vitro expression
title_short Cloning of DNA oligo pools for in vitro expression
title_sort cloning of dna oligo pools for in vitro expression
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9019715/
https://www.ncbi.nlm.nih.gov/pubmed/35462793
http://dx.doi.org/10.1016/j.xpro.2021.101103
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