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Preparation of BMP-2/PDA-BCP Bioceramic Scaffold by DLP 3D Printing and its Ability for Inducing Continuous Bone Formation

Digital light processing (DLP)-based 3D printing is suitable to fabricate bone scaffolds with small size and high precision. However, the published literature mainly deals with the fabrication procedure and parameters of DLP printed bioceramic scaffold, but lacks the subsequent systematic biological...

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Autores principales: Yang, Ziyang, Xie, Li, Zhang, Boqing, Zhang, Gang, Huo, Fangjun, Zhou, Changchun, Liang, Xi, Fan, Yujiang, Tian, Weidong, Tan, Yinghui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9019734/
https://www.ncbi.nlm.nih.gov/pubmed/35464724
http://dx.doi.org/10.3389/fbioe.2022.854693
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author Yang, Ziyang
Xie, Li
Zhang, Boqing
Zhang, Gang
Huo, Fangjun
Zhou, Changchun
Liang, Xi
Fan, Yujiang
Tian, Weidong
Tan, Yinghui
author_facet Yang, Ziyang
Xie, Li
Zhang, Boqing
Zhang, Gang
Huo, Fangjun
Zhou, Changchun
Liang, Xi
Fan, Yujiang
Tian, Weidong
Tan, Yinghui
author_sort Yang, Ziyang
collection PubMed
description Digital light processing (DLP)-based 3D printing is suitable to fabricate bone scaffolds with small size and high precision. However, the published literature mainly deals with the fabrication procedure and parameters of DLP printed bioceramic scaffold, but lacks the subsequent systematic biological evaluations for bone regeneration application. In this work, a biphasic calcium phosphate (BCP) macroporous scaffold was constructed by DLP-based 3D printing technique. Furthermore, bone morphogenetic protein-2 (BMP-2) was facilely incorporated into this scaffold through a facile polydopamine (PDA) modification process. The resultant scaffold presents an interconnected porous structure with pore size of ∼570 μm, compressive strength (∼3.6 MPa), and the self-assembly Ca-P/PDA nanocoating exhibited excellent sustained-release property for BMP-2. Notably, this BMP-2/PDA-BCP scaffold presents favorable effects on the adhesion, proliferation, osteogenic differentiation, and mineralization of bone marrow stromal cells (BMSCs). Furthermore, in vivo experiments conducted on rats demonstrated that the scaffolds could induce cell layer aggregation adjacent to the scaffolds and continuous new bone generation within the scaffold. Collectively, this work demonstrated that the BMP-2/PDA-BCP scaffold is of immense potential to treat small craniofacial bone defects in demand of high accuracy.
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spelling pubmed-90197342022-04-21 Preparation of BMP-2/PDA-BCP Bioceramic Scaffold by DLP 3D Printing and its Ability for Inducing Continuous Bone Formation Yang, Ziyang Xie, Li Zhang, Boqing Zhang, Gang Huo, Fangjun Zhou, Changchun Liang, Xi Fan, Yujiang Tian, Weidong Tan, Yinghui Front Bioeng Biotechnol Bioengineering and Biotechnology Digital light processing (DLP)-based 3D printing is suitable to fabricate bone scaffolds with small size and high precision. However, the published literature mainly deals with the fabrication procedure and parameters of DLP printed bioceramic scaffold, but lacks the subsequent systematic biological evaluations for bone regeneration application. In this work, a biphasic calcium phosphate (BCP) macroporous scaffold was constructed by DLP-based 3D printing technique. Furthermore, bone morphogenetic protein-2 (BMP-2) was facilely incorporated into this scaffold through a facile polydopamine (PDA) modification process. The resultant scaffold presents an interconnected porous structure with pore size of ∼570 μm, compressive strength (∼3.6 MPa), and the self-assembly Ca-P/PDA nanocoating exhibited excellent sustained-release property for BMP-2. Notably, this BMP-2/PDA-BCP scaffold presents favorable effects on the adhesion, proliferation, osteogenic differentiation, and mineralization of bone marrow stromal cells (BMSCs). Furthermore, in vivo experiments conducted on rats demonstrated that the scaffolds could induce cell layer aggregation adjacent to the scaffolds and continuous new bone generation within the scaffold. Collectively, this work demonstrated that the BMP-2/PDA-BCP scaffold is of immense potential to treat small craniofacial bone defects in demand of high accuracy. Frontiers Media S.A. 2022-04-06 /pmc/articles/PMC9019734/ /pubmed/35464724 http://dx.doi.org/10.3389/fbioe.2022.854693 Text en Copyright © 2022 Yang, Xie, Zhang, Zhang, Huo, Zhou, Liang, Fan, Tian and Tan. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Yang, Ziyang
Xie, Li
Zhang, Boqing
Zhang, Gang
Huo, Fangjun
Zhou, Changchun
Liang, Xi
Fan, Yujiang
Tian, Weidong
Tan, Yinghui
Preparation of BMP-2/PDA-BCP Bioceramic Scaffold by DLP 3D Printing and its Ability for Inducing Continuous Bone Formation
title Preparation of BMP-2/PDA-BCP Bioceramic Scaffold by DLP 3D Printing and its Ability for Inducing Continuous Bone Formation
title_full Preparation of BMP-2/PDA-BCP Bioceramic Scaffold by DLP 3D Printing and its Ability for Inducing Continuous Bone Formation
title_fullStr Preparation of BMP-2/PDA-BCP Bioceramic Scaffold by DLP 3D Printing and its Ability for Inducing Continuous Bone Formation
title_full_unstemmed Preparation of BMP-2/PDA-BCP Bioceramic Scaffold by DLP 3D Printing and its Ability for Inducing Continuous Bone Formation
title_short Preparation of BMP-2/PDA-BCP Bioceramic Scaffold by DLP 3D Printing and its Ability for Inducing Continuous Bone Formation
title_sort preparation of bmp-2/pda-bcp bioceramic scaffold by dlp 3d printing and its ability for inducing continuous bone formation
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9019734/
https://www.ncbi.nlm.nih.gov/pubmed/35464724
http://dx.doi.org/10.3389/fbioe.2022.854693
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