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Hematological and Histopathological Effects of Subacute Aconitine Poisoning in Mouse

Aconitine is the principal toxic ingredient of Aconitum, which can cause systemic poisoning involving multiple organs and systems after animal ingestion. The purpose of this study was to investigate the effects of aconitine on hematological indices and histological changes in mice. One hundred twent...

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Autores principales: Lu, Hao, Mei, Li, Guo, Ziyu, Wu, Kexin, Zhang, Yunhao, Tang, Shiyu, Zhu, Yiru, Zhao, Baoyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9020262/
https://www.ncbi.nlm.nih.gov/pubmed/35464374
http://dx.doi.org/10.3389/fvets.2022.874660
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author Lu, Hao
Mei, Li
Guo, Ziyu
Wu, Kexin
Zhang, Yunhao
Tang, Shiyu
Zhu, Yiru
Zhao, Baoyu
author_facet Lu, Hao
Mei, Li
Guo, Ziyu
Wu, Kexin
Zhang, Yunhao
Tang, Shiyu
Zhu, Yiru
Zhao, Baoyu
author_sort Lu, Hao
collection PubMed
description Aconitine is the principal toxic ingredient of Aconitum, which can cause systemic poisoning involving multiple organs and systems after animal ingestion. The purpose of this study was to investigate the effects of aconitine on hematological indices and histological changes in mice. One hundred twenty mice were divided into a control group (normal saline), low-dose group (0.14 μmol/L), middle-dose group (0.28 μmol/L) and high-dose group (0.56 μmol/L), which were continuously lavaged for 30 days. The blood of 10 mice were collected randomly and analyzed by group at the 10th, 20th, and 30th days, and some tissues were collected and stained with hematoxylin-eosin to observe histological changes at the 30th day. Compared with the control group, the organ coefficient (%) of liver, spleen, lungs, and brain of the high-dose group were significantly increased (p < 0.05 or p < 0.01). WBC and Gran initially decreased and then increased in each poisoning group, with significant differences in the high-dose group (p < 0.05 or p < 0.01). RBC, HGB, HCT, and PLT decreased continuously in all groups except the low-dose group at the 20th and 30th days (p < 0.05 or p < 0.01). Moreover, BUN, ALT and AST increased in each poisoning group, in comparison with the control group, with significant differences except for the low-dose group (p < 0.05 or p < 0.01). CRE initially increased and then decreased, the TP and ALB decreased, with significant differences observed in the high-dose and middle-dose groups (p < 0.05). All the mice in the poison-treated groups showed varying degrees of histopathological changes such as degeneration and necrosis of tissues, especially heart and cerebellum. Our data suggest that different doses of aconitine have remarkable effects on hematological and histopathological changes in mice, in a significant time and dose-effect relationship.
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spelling pubmed-90202622022-04-21 Hematological and Histopathological Effects of Subacute Aconitine Poisoning in Mouse Lu, Hao Mei, Li Guo, Ziyu Wu, Kexin Zhang, Yunhao Tang, Shiyu Zhu, Yiru Zhao, Baoyu Front Vet Sci Veterinary Science Aconitine is the principal toxic ingredient of Aconitum, which can cause systemic poisoning involving multiple organs and systems after animal ingestion. The purpose of this study was to investigate the effects of aconitine on hematological indices and histological changes in mice. One hundred twenty mice were divided into a control group (normal saline), low-dose group (0.14 μmol/L), middle-dose group (0.28 μmol/L) and high-dose group (0.56 μmol/L), which were continuously lavaged for 30 days. The blood of 10 mice were collected randomly and analyzed by group at the 10th, 20th, and 30th days, and some tissues were collected and stained with hematoxylin-eosin to observe histological changes at the 30th day. Compared with the control group, the organ coefficient (%) of liver, spleen, lungs, and brain of the high-dose group were significantly increased (p < 0.05 or p < 0.01). WBC and Gran initially decreased and then increased in each poisoning group, with significant differences in the high-dose group (p < 0.05 or p < 0.01). RBC, HGB, HCT, and PLT decreased continuously in all groups except the low-dose group at the 20th and 30th days (p < 0.05 or p < 0.01). Moreover, BUN, ALT and AST increased in each poisoning group, in comparison with the control group, with significant differences except for the low-dose group (p < 0.05 or p < 0.01). CRE initially increased and then decreased, the TP and ALB decreased, with significant differences observed in the high-dose and middle-dose groups (p < 0.05). All the mice in the poison-treated groups showed varying degrees of histopathological changes such as degeneration and necrosis of tissues, especially heart and cerebellum. Our data suggest that different doses of aconitine have remarkable effects on hematological and histopathological changes in mice, in a significant time and dose-effect relationship. Frontiers Media S.A. 2022-04-05 /pmc/articles/PMC9020262/ /pubmed/35464374 http://dx.doi.org/10.3389/fvets.2022.874660 Text en Copyright © 2022 Lu, Mei, Guo, Wu, Zhang, Tang, Zhu and Zhao. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Lu, Hao
Mei, Li
Guo, Ziyu
Wu, Kexin
Zhang, Yunhao
Tang, Shiyu
Zhu, Yiru
Zhao, Baoyu
Hematological and Histopathological Effects of Subacute Aconitine Poisoning in Mouse
title Hematological and Histopathological Effects of Subacute Aconitine Poisoning in Mouse
title_full Hematological and Histopathological Effects of Subacute Aconitine Poisoning in Mouse
title_fullStr Hematological and Histopathological Effects of Subacute Aconitine Poisoning in Mouse
title_full_unstemmed Hematological and Histopathological Effects of Subacute Aconitine Poisoning in Mouse
title_short Hematological and Histopathological Effects of Subacute Aconitine Poisoning in Mouse
title_sort hematological and histopathological effects of subacute aconitine poisoning in mouse
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9020262/
https://www.ncbi.nlm.nih.gov/pubmed/35464374
http://dx.doi.org/10.3389/fvets.2022.874660
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