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An in vitro Evaluation of Efficacy of ViaSpan, Aloe vera, Gatorade Solution, and Propolis Storage Media for Maintaining the Periodontal Ligament Cell Viability

BACKGROUND: Replantation is a commonly performed method for avulsed tooth. A vital periodontal membrane (periodontal ligament [PDL]) is significant for the successful healing of replanted teeth. Hence, various storage media are used to preserve the viability of periodontal cells before replantation....

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Autores principales: Misurya, Rajat, Sharma, Sandeep, Syed Ismail, Prabu Mahin, Gupta, Nitika, Rajan, Reshma, Kaur, Rasveen, Babaji, Prashant
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer - Medknow 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9020624/
https://www.ncbi.nlm.nih.gov/pubmed/35313402
http://dx.doi.org/10.4103/aam.aam_71_20
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author Misurya, Rajat
Sharma, Sandeep
Syed Ismail, Prabu Mahin
Gupta, Nitika
Rajan, Reshma
Kaur, Rasveen
Babaji, Prashant
author_facet Misurya, Rajat
Sharma, Sandeep
Syed Ismail, Prabu Mahin
Gupta, Nitika
Rajan, Reshma
Kaur, Rasveen
Babaji, Prashant
author_sort Misurya, Rajat
collection PubMed
description BACKGROUND: Replantation is a commonly performed method for avulsed tooth. A vital periodontal membrane (periodontal ligament [PDL]) is significant for the successful healing of replanted teeth. Hence, various storage media are used to preserve the viability of periodontal cells before replantation. OBJECTIVES: The present study was conducted to evaluate the efficacy of ViaSpan, Aloe vera, Gatorade solution, and propolis storage media for maintaining the PDL cell viability. MATERIALS AND METHODS: The present study was conducted on 40 recently extracted teeth which were randomly divided into four study storage groups: Group I: ViaSpan, Group II: Aloe vera, Group III: Gatorade solution, and Group IV: Propolis. Later they were subjected to centrifugation, and the cells from supernatant were colored with 0.4% trypan blue for determination of viability. The obtained data were statistically evaluated with SPSS package (21.0 version, Inc.; Chicago, IL, USA) using analysis of variance, Mann–Whitney test, and Post hoc tests. RESULTS: The mean viable periodontal cell in Group I was 30.2 cumm, in Group II was 24.6 cumm, Group III was 14.5 cumm, and Group IV in 31.4. The difference was significant (P < 0.01). Post hoc test between different groups revealed a significant difference in mean viable periodontal cells (P < 0.001). Propolis, ViaSpan, and Aloe vera had higher pH and osmolality values. CONCLUSION: This study found that propolis had higher periodontal cell viability followed by ViaSpan solution and Aloe vera and least in Gatorade solution. Propolis, ViaSpan, and Aloe vera media can be used as a storage media.
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spelling pubmed-90206242022-04-21 An in vitro Evaluation of Efficacy of ViaSpan, Aloe vera, Gatorade Solution, and Propolis Storage Media for Maintaining the Periodontal Ligament Cell Viability Misurya, Rajat Sharma, Sandeep Syed Ismail, Prabu Mahin Gupta, Nitika Rajan, Reshma Kaur, Rasveen Babaji, Prashant Ann Afr Med Original Article BACKGROUND: Replantation is a commonly performed method for avulsed tooth. A vital periodontal membrane (periodontal ligament [PDL]) is significant for the successful healing of replanted teeth. Hence, various storage media are used to preserve the viability of periodontal cells before replantation. OBJECTIVES: The present study was conducted to evaluate the efficacy of ViaSpan, Aloe vera, Gatorade solution, and propolis storage media for maintaining the PDL cell viability. MATERIALS AND METHODS: The present study was conducted on 40 recently extracted teeth which were randomly divided into four study storage groups: Group I: ViaSpan, Group II: Aloe vera, Group III: Gatorade solution, and Group IV: Propolis. Later they were subjected to centrifugation, and the cells from supernatant were colored with 0.4% trypan blue for determination of viability. The obtained data were statistically evaluated with SPSS package (21.0 version, Inc.; Chicago, IL, USA) using analysis of variance, Mann–Whitney test, and Post hoc tests. RESULTS: The mean viable periodontal cell in Group I was 30.2 cumm, in Group II was 24.6 cumm, Group III was 14.5 cumm, and Group IV in 31.4. The difference was significant (P < 0.01). Post hoc test between different groups revealed a significant difference in mean viable periodontal cells (P < 0.001). Propolis, ViaSpan, and Aloe vera had higher pH and osmolality values. CONCLUSION: This study found that propolis had higher periodontal cell viability followed by ViaSpan solution and Aloe vera and least in Gatorade solution. Propolis, ViaSpan, and Aloe vera media can be used as a storage media. Wolters Kluwer - Medknow 2022 2022-03-18 /pmc/articles/PMC9020624/ /pubmed/35313402 http://dx.doi.org/10.4103/aam.aam_71_20 Text en Copyright: © 2022 Annals of African Medicine https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Misurya, Rajat
Sharma, Sandeep
Syed Ismail, Prabu Mahin
Gupta, Nitika
Rajan, Reshma
Kaur, Rasveen
Babaji, Prashant
An in vitro Evaluation of Efficacy of ViaSpan, Aloe vera, Gatorade Solution, and Propolis Storage Media for Maintaining the Periodontal Ligament Cell Viability
title An in vitro Evaluation of Efficacy of ViaSpan, Aloe vera, Gatorade Solution, and Propolis Storage Media for Maintaining the Periodontal Ligament Cell Viability
title_full An in vitro Evaluation of Efficacy of ViaSpan, Aloe vera, Gatorade Solution, and Propolis Storage Media for Maintaining the Periodontal Ligament Cell Viability
title_fullStr An in vitro Evaluation of Efficacy of ViaSpan, Aloe vera, Gatorade Solution, and Propolis Storage Media for Maintaining the Periodontal Ligament Cell Viability
title_full_unstemmed An in vitro Evaluation of Efficacy of ViaSpan, Aloe vera, Gatorade Solution, and Propolis Storage Media for Maintaining the Periodontal Ligament Cell Viability
title_short An in vitro Evaluation of Efficacy of ViaSpan, Aloe vera, Gatorade Solution, and Propolis Storage Media for Maintaining the Periodontal Ligament Cell Viability
title_sort in vitro evaluation of efficacy of viaspan, aloe vera, gatorade solution, and propolis storage media for maintaining the periodontal ligament cell viability
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9020624/
https://www.ncbi.nlm.nih.gov/pubmed/35313402
http://dx.doi.org/10.4103/aam.aam_71_20
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