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Ad26.COV2.S prevents upregulation of SARS-CoV-2 induced pathways of inflammation and thrombosis in hamsters and rhesus macaques
Syrian golden hamsters exhibit features of severe disease after SARS-CoV-2 WA1/2020 challenge and are therefore useful models of COVID-19 pathogenesis and prevention with vaccines. Recent studies have shown that SARS-CoV-2 infection stimulates type I interferon, myeloid, and inflammatory signatures...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9020736/ https://www.ncbi.nlm.nih.gov/pubmed/35395058 http://dx.doi.org/10.1371/journal.ppat.1009990 |
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author | Aid, Malika Vidal, Samuel J. Piedra-Mora, Cesar Ducat, Sarah Chan, Chi N. Bondoc, Stephen Colarusso, Alessandro Starke, Carly E. Nekorchuk, Michael Busman-Sahay, Kathleen Estes, Jacob D. Martinot, Amanda J. Barouch, Dan H. |
author_facet | Aid, Malika Vidal, Samuel J. Piedra-Mora, Cesar Ducat, Sarah Chan, Chi N. Bondoc, Stephen Colarusso, Alessandro Starke, Carly E. Nekorchuk, Michael Busman-Sahay, Kathleen Estes, Jacob D. Martinot, Amanda J. Barouch, Dan H. |
author_sort | Aid, Malika |
collection | PubMed |
description | Syrian golden hamsters exhibit features of severe disease after SARS-CoV-2 WA1/2020 challenge and are therefore useful models of COVID-19 pathogenesis and prevention with vaccines. Recent studies have shown that SARS-CoV-2 infection stimulates type I interferon, myeloid, and inflammatory signatures similar to human disease and that weight loss can be prevented with vaccines. However, the impact of vaccination on transcriptional programs associated with COVID-19 pathogenesis and protective adaptive immune responses is unknown. Here we show that SARS-CoV-2 WA1/2020 challenge in hamsters stimulates myeloid and inflammatory programs as well as signatures of complement and thrombosis associated with human COVID-19. Notably, immunization with Ad26.COV2.S, an adenovirus serotype 26 vector (Ad26)-based vaccine expressing a stabilized SARS-CoV-2 spike protein, prevents the upregulation of these pathways, such that the mRNA expression profiles of vaccinated hamsters are comparable to uninfected animals. Using proteomics profiling, we validated these findings in rhesus macaques challenged with SARS-CoV-2 WA1/2020 or SARS-CoV-2 B.1.351. Finally, we show that Ad26.COV2.S vaccination induces T and B cell signatures that correlate with binding and neutralizing antibody responses weeks following vaccination. These data provide insights into the molecular mechanisms of Ad26.COV2.S protection against severe COVID-19 in animal models. |
format | Online Article Text |
id | pubmed-9020736 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-90207362022-04-21 Ad26.COV2.S prevents upregulation of SARS-CoV-2 induced pathways of inflammation and thrombosis in hamsters and rhesus macaques Aid, Malika Vidal, Samuel J. Piedra-Mora, Cesar Ducat, Sarah Chan, Chi N. Bondoc, Stephen Colarusso, Alessandro Starke, Carly E. Nekorchuk, Michael Busman-Sahay, Kathleen Estes, Jacob D. Martinot, Amanda J. Barouch, Dan H. PLoS Pathog Research Article Syrian golden hamsters exhibit features of severe disease after SARS-CoV-2 WA1/2020 challenge and are therefore useful models of COVID-19 pathogenesis and prevention with vaccines. Recent studies have shown that SARS-CoV-2 infection stimulates type I interferon, myeloid, and inflammatory signatures similar to human disease and that weight loss can be prevented with vaccines. However, the impact of vaccination on transcriptional programs associated with COVID-19 pathogenesis and protective adaptive immune responses is unknown. Here we show that SARS-CoV-2 WA1/2020 challenge in hamsters stimulates myeloid and inflammatory programs as well as signatures of complement and thrombosis associated with human COVID-19. Notably, immunization with Ad26.COV2.S, an adenovirus serotype 26 vector (Ad26)-based vaccine expressing a stabilized SARS-CoV-2 spike protein, prevents the upregulation of these pathways, such that the mRNA expression profiles of vaccinated hamsters are comparable to uninfected animals. Using proteomics profiling, we validated these findings in rhesus macaques challenged with SARS-CoV-2 WA1/2020 or SARS-CoV-2 B.1.351. Finally, we show that Ad26.COV2.S vaccination induces T and B cell signatures that correlate with binding and neutralizing antibody responses weeks following vaccination. These data provide insights into the molecular mechanisms of Ad26.COV2.S protection against severe COVID-19 in animal models. Public Library of Science 2022-04-08 /pmc/articles/PMC9020736/ /pubmed/35395058 http://dx.doi.org/10.1371/journal.ppat.1009990 Text en © 2022 Aid et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Aid, Malika Vidal, Samuel J. Piedra-Mora, Cesar Ducat, Sarah Chan, Chi N. Bondoc, Stephen Colarusso, Alessandro Starke, Carly E. Nekorchuk, Michael Busman-Sahay, Kathleen Estes, Jacob D. Martinot, Amanda J. Barouch, Dan H. Ad26.COV2.S prevents upregulation of SARS-CoV-2 induced pathways of inflammation and thrombosis in hamsters and rhesus macaques |
title | Ad26.COV2.S prevents upregulation of SARS-CoV-2 induced pathways of inflammation and thrombosis in hamsters and rhesus macaques |
title_full | Ad26.COV2.S prevents upregulation of SARS-CoV-2 induced pathways of inflammation and thrombosis in hamsters and rhesus macaques |
title_fullStr | Ad26.COV2.S prevents upregulation of SARS-CoV-2 induced pathways of inflammation and thrombosis in hamsters and rhesus macaques |
title_full_unstemmed | Ad26.COV2.S prevents upregulation of SARS-CoV-2 induced pathways of inflammation and thrombosis in hamsters and rhesus macaques |
title_short | Ad26.COV2.S prevents upregulation of SARS-CoV-2 induced pathways of inflammation and thrombosis in hamsters and rhesus macaques |
title_sort | ad26.cov2.s prevents upregulation of sars-cov-2 induced pathways of inflammation and thrombosis in hamsters and rhesus macaques |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9020736/ https://www.ncbi.nlm.nih.gov/pubmed/35395058 http://dx.doi.org/10.1371/journal.ppat.1009990 |
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