Cargando…

Aberrant Expression of Mitochondrial SAM Transporter SLC25A26 Impairs Oocyte Maturation and Early Development in Mice

The immature germinal vesicle (GV) oocytes proceed through metaphase I (MI) division, extrude the first polar body, and become mature metaphase II (MII) oocytes for fertilization which is followed by preimplantation and postimplantation development until birth. Slc25a26 is the gene encoding S-adenos...

Descripción completa

Detalles Bibliográficos
Autores principales: Cheng, Gui-ping, Guo, Shi-meng, Yin, Ying, Li, Yuan-yuan, He, Ximiao, Zhou, Li-quan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9020962/
https://www.ncbi.nlm.nih.gov/pubmed/35464759
http://dx.doi.org/10.1155/2022/1681623
_version_ 1784689687975165952
author Cheng, Gui-ping
Guo, Shi-meng
Yin, Ying
Li, Yuan-yuan
He, Ximiao
Zhou, Li-quan
author_facet Cheng, Gui-ping
Guo, Shi-meng
Yin, Ying
Li, Yuan-yuan
He, Ximiao
Zhou, Li-quan
author_sort Cheng, Gui-ping
collection PubMed
description The immature germinal vesicle (GV) oocytes proceed through metaphase I (MI) division, extrude the first polar body, and become mature metaphase II (MII) oocytes for fertilization which is followed by preimplantation and postimplantation development until birth. Slc25a26 is the gene encoding S-adenosylmethionine carrier (SAMC), a member of the mitochondrial carrier family. Its major function is to catalyze the uptake of S-adenosylmethionine (SAM) from cytosol into mitochondria, which is the only known mitochondrial SAM transporter. In the present study, we demonstrated that excessive SLC25A26 accumulation in mouse oocytes mimicked naturally aged oocytes and resulted in lower oocyte quality with decreased maturation rate and increased reactive oxygen species (ROS) by impairing mitochondrial function. Increased level of Slc25a26 gene impacted gene expression in mouse oocytes such as mt-Cytb which regulates mitochondrial respiratory chain. Furthermore, increased level of Slc25a26 gene in fertilized oocytes slightly compromised blastocyst formation, and Slc25a26 knockout mice displayed embryonic lethality around 10.5 dpc. Taken together, our results showed that Slc25a26 gene plays a critical role in oocyte maturation and early mouse development.
format Online
Article
Text
id pubmed-9020962
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-90209622022-04-21 Aberrant Expression of Mitochondrial SAM Transporter SLC25A26 Impairs Oocyte Maturation and Early Development in Mice Cheng, Gui-ping Guo, Shi-meng Yin, Ying Li, Yuan-yuan He, Ximiao Zhou, Li-quan Oxid Med Cell Longev Research Article The immature germinal vesicle (GV) oocytes proceed through metaphase I (MI) division, extrude the first polar body, and become mature metaphase II (MII) oocytes for fertilization which is followed by preimplantation and postimplantation development until birth. Slc25a26 is the gene encoding S-adenosylmethionine carrier (SAMC), a member of the mitochondrial carrier family. Its major function is to catalyze the uptake of S-adenosylmethionine (SAM) from cytosol into mitochondria, which is the only known mitochondrial SAM transporter. In the present study, we demonstrated that excessive SLC25A26 accumulation in mouse oocytes mimicked naturally aged oocytes and resulted in lower oocyte quality with decreased maturation rate and increased reactive oxygen species (ROS) by impairing mitochondrial function. Increased level of Slc25a26 gene impacted gene expression in mouse oocytes such as mt-Cytb which regulates mitochondrial respiratory chain. Furthermore, increased level of Slc25a26 gene in fertilized oocytes slightly compromised blastocyst formation, and Slc25a26 knockout mice displayed embryonic lethality around 10.5 dpc. Taken together, our results showed that Slc25a26 gene plays a critical role in oocyte maturation and early mouse development. Hindawi 2022-04-13 /pmc/articles/PMC9020962/ /pubmed/35464759 http://dx.doi.org/10.1155/2022/1681623 Text en Copyright © 2022 Gui-ping Cheng et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Cheng, Gui-ping
Guo, Shi-meng
Yin, Ying
Li, Yuan-yuan
He, Ximiao
Zhou, Li-quan
Aberrant Expression of Mitochondrial SAM Transporter SLC25A26 Impairs Oocyte Maturation and Early Development in Mice
title Aberrant Expression of Mitochondrial SAM Transporter SLC25A26 Impairs Oocyte Maturation and Early Development in Mice
title_full Aberrant Expression of Mitochondrial SAM Transporter SLC25A26 Impairs Oocyte Maturation and Early Development in Mice
title_fullStr Aberrant Expression of Mitochondrial SAM Transporter SLC25A26 Impairs Oocyte Maturation and Early Development in Mice
title_full_unstemmed Aberrant Expression of Mitochondrial SAM Transporter SLC25A26 Impairs Oocyte Maturation and Early Development in Mice
title_short Aberrant Expression of Mitochondrial SAM Transporter SLC25A26 Impairs Oocyte Maturation and Early Development in Mice
title_sort aberrant expression of mitochondrial sam transporter slc25a26 impairs oocyte maturation and early development in mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9020962/
https://www.ncbi.nlm.nih.gov/pubmed/35464759
http://dx.doi.org/10.1155/2022/1681623
work_keys_str_mv AT chengguiping aberrantexpressionofmitochondrialsamtransporterslc25a26impairsoocytematurationandearlydevelopmentinmice
AT guoshimeng aberrantexpressionofmitochondrialsamtransporterslc25a26impairsoocytematurationandearlydevelopmentinmice
AT yinying aberrantexpressionofmitochondrialsamtransporterslc25a26impairsoocytematurationandearlydevelopmentinmice
AT liyuanyuan aberrantexpressionofmitochondrialsamtransporterslc25a26impairsoocytematurationandearlydevelopmentinmice
AT heximiao aberrantexpressionofmitochondrialsamtransporterslc25a26impairsoocytematurationandearlydevelopmentinmice
AT zhouliquan aberrantexpressionofmitochondrialsamtransporterslc25a26impairsoocytematurationandearlydevelopmentinmice