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Adaptive Evolution of the Fox Coronavirus Based on Genome-Wide Sequence Analysis

PURPOSE: To report the first complete fox coronavirus (CoV) genome sequence obtained through genome-wide amplifications and to understand the adaptive evolution of fox CoV. METHODS: Anal swab samples were collected from 35 foxes to detect the presence of CoV and obtain the virus sequence. Phylogenet...

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Autores principales: Feng, Chunyu, Liu, Yuting, Lyu, Guangqi, Shang, Songyang, Xia, Hongyue, Zhang, Junpeng, Irwin, David M., Wang, Zhe, Zhang, Shuyi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9020971/
https://www.ncbi.nlm.nih.gov/pubmed/35463975
http://dx.doi.org/10.1155/2022/9627961
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author Feng, Chunyu
Liu, Yuting
Lyu, Guangqi
Shang, Songyang
Xia, Hongyue
Zhang, Junpeng
Irwin, David M.
Wang, Zhe
Zhang, Shuyi
author_facet Feng, Chunyu
Liu, Yuting
Lyu, Guangqi
Shang, Songyang
Xia, Hongyue
Zhang, Junpeng
Irwin, David M.
Wang, Zhe
Zhang, Shuyi
author_sort Feng, Chunyu
collection PubMed
description PURPOSE: To report the first complete fox coronavirus (CoV) genome sequence obtained through genome-wide amplifications and to understand the adaptive evolution of fox CoV. METHODS: Anal swab samples were collected from 35 foxes to detect the presence of CoV and obtain the virus sequence. Phylogenetic analysis was conducted using MrBayes. The possibility of recombination within these sequences was assessed using GARD. Analysis of the levels of selection pressure experienced by these sequences was assessed using methods on both the PAML and Data Monkey platforms. RESULTS: Of the 35 samples, two were positive, and complete genome sequences for the viruses were obtained. Phylogenetic analysis, using Bayesian methods, of these sequences, together with other CoV sequences, revealed that the fox CoV sequences clustered with canine coronavirus (CCoV) sequences, with sequences from other carnivores more distantly related. In contrast to the feline, ferret and mink CoV sequences that clustered into species-specific clades, the fox CoV fell within the CCoV clade. Minimal evidence for recombination was found among the sequences. A total of 7, 3, 14, and 2 positively selected sites were identified in the M, N, S, and 7B genes, respectively, with 99, 111, and 581 negatively selected sites identified in M, N, and S genes, respectively. CONCLUSION: The complete genome sequence of fox CoV has been obtained for the first time. The results suggest that the genome sequence of fox CoV may have experienced adaptive evolution in the genes replication, entry, and virulence. The number of sites in each gene that experienced negative selection is far greater than the number that underwent positive selection, suggesting that most of the sequence is highly conserved and important for viral survive. However, positive selection at a few sites likely aided these viruses to adapt to new environments.
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spelling pubmed-90209712022-04-21 Adaptive Evolution of the Fox Coronavirus Based on Genome-Wide Sequence Analysis Feng, Chunyu Liu, Yuting Lyu, Guangqi Shang, Songyang Xia, Hongyue Zhang, Junpeng Irwin, David M. Wang, Zhe Zhang, Shuyi Biomed Res Int Research Article PURPOSE: To report the first complete fox coronavirus (CoV) genome sequence obtained through genome-wide amplifications and to understand the adaptive evolution of fox CoV. METHODS: Anal swab samples were collected from 35 foxes to detect the presence of CoV and obtain the virus sequence. Phylogenetic analysis was conducted using MrBayes. The possibility of recombination within these sequences was assessed using GARD. Analysis of the levels of selection pressure experienced by these sequences was assessed using methods on both the PAML and Data Monkey platforms. RESULTS: Of the 35 samples, two were positive, and complete genome sequences for the viruses were obtained. Phylogenetic analysis, using Bayesian methods, of these sequences, together with other CoV sequences, revealed that the fox CoV sequences clustered with canine coronavirus (CCoV) sequences, with sequences from other carnivores more distantly related. In contrast to the feline, ferret and mink CoV sequences that clustered into species-specific clades, the fox CoV fell within the CCoV clade. Minimal evidence for recombination was found among the sequences. A total of 7, 3, 14, and 2 positively selected sites were identified in the M, N, S, and 7B genes, respectively, with 99, 111, and 581 negatively selected sites identified in M, N, and S genes, respectively. CONCLUSION: The complete genome sequence of fox CoV has been obtained for the first time. The results suggest that the genome sequence of fox CoV may have experienced adaptive evolution in the genes replication, entry, and virulence. The number of sites in each gene that experienced negative selection is far greater than the number that underwent positive selection, suggesting that most of the sequence is highly conserved and important for viral survive. However, positive selection at a few sites likely aided these viruses to adapt to new environments. Hindawi 2022-04-13 /pmc/articles/PMC9020971/ /pubmed/35463975 http://dx.doi.org/10.1155/2022/9627961 Text en Copyright © 2022 Chunyu Feng et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Feng, Chunyu
Liu, Yuting
Lyu, Guangqi
Shang, Songyang
Xia, Hongyue
Zhang, Junpeng
Irwin, David M.
Wang, Zhe
Zhang, Shuyi
Adaptive Evolution of the Fox Coronavirus Based on Genome-Wide Sequence Analysis
title Adaptive Evolution of the Fox Coronavirus Based on Genome-Wide Sequence Analysis
title_full Adaptive Evolution of the Fox Coronavirus Based on Genome-Wide Sequence Analysis
title_fullStr Adaptive Evolution of the Fox Coronavirus Based on Genome-Wide Sequence Analysis
title_full_unstemmed Adaptive Evolution of the Fox Coronavirus Based on Genome-Wide Sequence Analysis
title_short Adaptive Evolution of the Fox Coronavirus Based on Genome-Wide Sequence Analysis
title_sort adaptive evolution of the fox coronavirus based on genome-wide sequence analysis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9020971/
https://www.ncbi.nlm.nih.gov/pubmed/35463975
http://dx.doi.org/10.1155/2022/9627961
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