Cargando…

The Potential of Metabolism-Related Gene OGDHL as a Biomarker for Myocardial Remodeling in Dilated Cardiomyopathy

The development of dilated cardiomyopathy (DCM) is accompanied by a series of metabolic disorders, resulting in myocardial remodeling or exacerbation, while the mechanism remains not completely clear. This study was to find out the key metabolism-related genes involved in the onset of DCM, providing...

Descripción completa

Detalles Bibliográficos
Autores principales: Tang, Yaohan, Zhu, Yaoxi, Lu, Yang, Yang, Hongmin, Yang, Han, Li, Lixia, Liu, Changhu, Du, Yimei, Yuan, Jing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9021392/
https://www.ncbi.nlm.nih.gov/pubmed/35463769
http://dx.doi.org/10.3389/fcvm.2022.741920
_version_ 1784689806204207104
author Tang, Yaohan
Zhu, Yaoxi
Lu, Yang
Yang, Hongmin
Yang, Han
Li, Lixia
Liu, Changhu
Du, Yimei
Yuan, Jing
author_facet Tang, Yaohan
Zhu, Yaoxi
Lu, Yang
Yang, Hongmin
Yang, Han
Li, Lixia
Liu, Changhu
Du, Yimei
Yuan, Jing
author_sort Tang, Yaohan
collection PubMed
description The development of dilated cardiomyopathy (DCM) is accompanied by a series of metabolic disorders, resulting in myocardial remodeling or exacerbation, while the mechanism remains not completely clear. This study was to find out the key metabolism-related genes involved in the onset of DCM, providing new insight into the pathogenesis of this disease. The datasets of GSE57338, GSE116250, and GSE5406 associated with hearts of patients with DCM were downloaded from the Gene Expression Omnibus database. GSE57338 was analyzed to screen out metabolism-related differentially expressed genes (DEGs), while GSE116250 and GSE5406 were utilized to verify the optimal genes through R software. Support vector machine recursive feature elimination algorithm and least absolute shrinkage and selection operator algorithm were used to determine key genes. Finally, 6 of 39 metabolism-related DEGs were screened out and identified as the optimal genes. After quantitative reverse-transcription polymerase chain reaction (qRT-PCR) validation performed on the samples drawn from the left ventricles of human hearts, it showed that only the expression of oxoglutarate dehydrogenase-like (OGDHL) increased while PLA2G2 decreased significantly in patients with DCM compared with non-failing donors, respectively. Furthermore, the higher OGDHL protein expression, except the change of PLA2G2, was also found in DCM hearts, and its mRNA expression was negatively correlated with myocardial Masson’s scores (r = –0.84, P = 0.009) and left ventricular end-diastolic diameter (LVEDd; r = –0.82, P = 0.014), which might be regulated by miR-3925-5p through further bioinformatics prediction and qRT-PCR verification. The data then suggested that the metabolism-related gene OGDHL was associated with myocardial fibrosis of DCM and probably a biomarker for myocardial remodeling in patients with DCM.
format Online
Article
Text
id pubmed-9021392
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-90213922022-04-22 The Potential of Metabolism-Related Gene OGDHL as a Biomarker for Myocardial Remodeling in Dilated Cardiomyopathy Tang, Yaohan Zhu, Yaoxi Lu, Yang Yang, Hongmin Yang, Han Li, Lixia Liu, Changhu Du, Yimei Yuan, Jing Front Cardiovasc Med Cardiovascular Medicine The development of dilated cardiomyopathy (DCM) is accompanied by a series of metabolic disorders, resulting in myocardial remodeling or exacerbation, while the mechanism remains not completely clear. This study was to find out the key metabolism-related genes involved in the onset of DCM, providing new insight into the pathogenesis of this disease. The datasets of GSE57338, GSE116250, and GSE5406 associated with hearts of patients with DCM were downloaded from the Gene Expression Omnibus database. GSE57338 was analyzed to screen out metabolism-related differentially expressed genes (DEGs), while GSE116250 and GSE5406 were utilized to verify the optimal genes through R software. Support vector machine recursive feature elimination algorithm and least absolute shrinkage and selection operator algorithm were used to determine key genes. Finally, 6 of 39 metabolism-related DEGs were screened out and identified as the optimal genes. After quantitative reverse-transcription polymerase chain reaction (qRT-PCR) validation performed on the samples drawn from the left ventricles of human hearts, it showed that only the expression of oxoglutarate dehydrogenase-like (OGDHL) increased while PLA2G2 decreased significantly in patients with DCM compared with non-failing donors, respectively. Furthermore, the higher OGDHL protein expression, except the change of PLA2G2, was also found in DCM hearts, and its mRNA expression was negatively correlated with myocardial Masson’s scores (r = –0.84, P = 0.009) and left ventricular end-diastolic diameter (LVEDd; r = –0.82, P = 0.014), which might be regulated by miR-3925-5p through further bioinformatics prediction and qRT-PCR verification. The data then suggested that the metabolism-related gene OGDHL was associated with myocardial fibrosis of DCM and probably a biomarker for myocardial remodeling in patients with DCM. Frontiers Media S.A. 2022-04-07 /pmc/articles/PMC9021392/ /pubmed/35463769 http://dx.doi.org/10.3389/fcvm.2022.741920 Text en Copyright © 2022 Tang, Zhu, Lu, Yang, Yang, Li, Liu, Du and Yuan. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cardiovascular Medicine
Tang, Yaohan
Zhu, Yaoxi
Lu, Yang
Yang, Hongmin
Yang, Han
Li, Lixia
Liu, Changhu
Du, Yimei
Yuan, Jing
The Potential of Metabolism-Related Gene OGDHL as a Biomarker for Myocardial Remodeling in Dilated Cardiomyopathy
title The Potential of Metabolism-Related Gene OGDHL as a Biomarker for Myocardial Remodeling in Dilated Cardiomyopathy
title_full The Potential of Metabolism-Related Gene OGDHL as a Biomarker for Myocardial Remodeling in Dilated Cardiomyopathy
title_fullStr The Potential of Metabolism-Related Gene OGDHL as a Biomarker for Myocardial Remodeling in Dilated Cardiomyopathy
title_full_unstemmed The Potential of Metabolism-Related Gene OGDHL as a Biomarker for Myocardial Remodeling in Dilated Cardiomyopathy
title_short The Potential of Metabolism-Related Gene OGDHL as a Biomarker for Myocardial Remodeling in Dilated Cardiomyopathy
title_sort potential of metabolism-related gene ogdhl as a biomarker for myocardial remodeling in dilated cardiomyopathy
topic Cardiovascular Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9021392/
https://www.ncbi.nlm.nih.gov/pubmed/35463769
http://dx.doi.org/10.3389/fcvm.2022.741920
work_keys_str_mv AT tangyaohan thepotentialofmetabolismrelatedgeneogdhlasabiomarkerformyocardialremodelingindilatedcardiomyopathy
AT zhuyaoxi thepotentialofmetabolismrelatedgeneogdhlasabiomarkerformyocardialremodelingindilatedcardiomyopathy
AT luyang thepotentialofmetabolismrelatedgeneogdhlasabiomarkerformyocardialremodelingindilatedcardiomyopathy
AT yanghongmin thepotentialofmetabolismrelatedgeneogdhlasabiomarkerformyocardialremodelingindilatedcardiomyopathy
AT yanghan thepotentialofmetabolismrelatedgeneogdhlasabiomarkerformyocardialremodelingindilatedcardiomyopathy
AT lilixia thepotentialofmetabolismrelatedgeneogdhlasabiomarkerformyocardialremodelingindilatedcardiomyopathy
AT liuchanghu thepotentialofmetabolismrelatedgeneogdhlasabiomarkerformyocardialremodelingindilatedcardiomyopathy
AT duyimei thepotentialofmetabolismrelatedgeneogdhlasabiomarkerformyocardialremodelingindilatedcardiomyopathy
AT yuanjing thepotentialofmetabolismrelatedgeneogdhlasabiomarkerformyocardialremodelingindilatedcardiomyopathy
AT tangyaohan potentialofmetabolismrelatedgeneogdhlasabiomarkerformyocardialremodelingindilatedcardiomyopathy
AT zhuyaoxi potentialofmetabolismrelatedgeneogdhlasabiomarkerformyocardialremodelingindilatedcardiomyopathy
AT luyang potentialofmetabolismrelatedgeneogdhlasabiomarkerformyocardialremodelingindilatedcardiomyopathy
AT yanghongmin potentialofmetabolismrelatedgeneogdhlasabiomarkerformyocardialremodelingindilatedcardiomyopathy
AT yanghan potentialofmetabolismrelatedgeneogdhlasabiomarkerformyocardialremodelingindilatedcardiomyopathy
AT lilixia potentialofmetabolismrelatedgeneogdhlasabiomarkerformyocardialremodelingindilatedcardiomyopathy
AT liuchanghu potentialofmetabolismrelatedgeneogdhlasabiomarkerformyocardialremodelingindilatedcardiomyopathy
AT duyimei potentialofmetabolismrelatedgeneogdhlasabiomarkerformyocardialremodelingindilatedcardiomyopathy
AT yuanjing potentialofmetabolismrelatedgeneogdhlasabiomarkerformyocardialremodelingindilatedcardiomyopathy