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Dichotomy in Neutralizing Antibody Induction to Peptide-Conjugated Vaccine in Squalene Emulsion Contrast With Aluminum Hydroxide Formulation
W614A-3S peptide is a modified 3S motif of the HIV-gp41 (mutation W614A). We previously detected the presence of natural neutralizing antibodies directed against W614A-3S peptide (NAbs) in long-term non-progressor HIV(+) patients. Here, we compared the efficacy of W614A-3S peptide formulated in eith...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9021396/ https://www.ncbi.nlm.nih.gov/pubmed/35464449 http://dx.doi.org/10.3389/fimmu.2022.848571 |
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author | Bonduelle, Olivia Chaudesaigues, Chloé Tolazzi, Monica Suleiman, Ehsan de Bernard, Simon Alves, Karine Nourikyan, Julien Bohec, Mylene Baudrin, Laura G. Katinger, Dietmar Debré, Patrice Scarlatti, Gabriella Vieillard, Vincent Combadière, Behazine |
author_facet | Bonduelle, Olivia Chaudesaigues, Chloé Tolazzi, Monica Suleiman, Ehsan de Bernard, Simon Alves, Karine Nourikyan, Julien Bohec, Mylene Baudrin, Laura G. Katinger, Dietmar Debré, Patrice Scarlatti, Gabriella Vieillard, Vincent Combadière, Behazine |
author_sort | Bonduelle, Olivia |
collection | PubMed |
description | W614A-3S peptide is a modified 3S motif of the HIV-gp41 (mutation W614A). We previously detected the presence of natural neutralizing antibodies directed against W614A-3S peptide (NAbs) in long-term non-progressor HIV(+) patients. Here, we compared the efficacy of W614A-3S peptide formulated in either squalene emulsion (SQE) or in aluminum hydroxide (Alum) in inducing broadly-NAbs (bNAbs). Rabbit and mouse models were used to screen the induction of bNAbs following 4 immunizations. SQE was more efficient than Alum formulation in inducing W614A-3S-specific bNAbs with up to 67%–93% of HIV strains neutralized. We then analyzed the quality of peptide-specific murine B cells by single-cell gene expression by quantitative reverse transcription-PCR and single-cell V(D)J sequencing. We found more frequent germinal center B cells in SQE than in Alum, albeit with a different gene expression profile. The V(D)J sequencing of W614A-3S-specific BCR showed significant differences in BCR sequences and validates the dichotomy between adjuvant formulations. All sixteen BCR sequences which were cloned were specific of peptide. Adjuvant formulations of W614A-3S-peptide-conjugated immunogen impact the quantity and quality of B cell immune responses at both the gene expression level and BCR sequence. |
format | Online Article Text |
id | pubmed-9021396 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-90213962022-04-22 Dichotomy in Neutralizing Antibody Induction to Peptide-Conjugated Vaccine in Squalene Emulsion Contrast With Aluminum Hydroxide Formulation Bonduelle, Olivia Chaudesaigues, Chloé Tolazzi, Monica Suleiman, Ehsan de Bernard, Simon Alves, Karine Nourikyan, Julien Bohec, Mylene Baudrin, Laura G. Katinger, Dietmar Debré, Patrice Scarlatti, Gabriella Vieillard, Vincent Combadière, Behazine Front Immunol Immunology W614A-3S peptide is a modified 3S motif of the HIV-gp41 (mutation W614A). We previously detected the presence of natural neutralizing antibodies directed against W614A-3S peptide (NAbs) in long-term non-progressor HIV(+) patients. Here, we compared the efficacy of W614A-3S peptide formulated in either squalene emulsion (SQE) or in aluminum hydroxide (Alum) in inducing broadly-NAbs (bNAbs). Rabbit and mouse models were used to screen the induction of bNAbs following 4 immunizations. SQE was more efficient than Alum formulation in inducing W614A-3S-specific bNAbs with up to 67%–93% of HIV strains neutralized. We then analyzed the quality of peptide-specific murine B cells by single-cell gene expression by quantitative reverse transcription-PCR and single-cell V(D)J sequencing. We found more frequent germinal center B cells in SQE than in Alum, albeit with a different gene expression profile. The V(D)J sequencing of W614A-3S-specific BCR showed significant differences in BCR sequences and validates the dichotomy between adjuvant formulations. All sixteen BCR sequences which were cloned were specific of peptide. Adjuvant formulations of W614A-3S-peptide-conjugated immunogen impact the quantity and quality of B cell immune responses at both the gene expression level and BCR sequence. Frontiers Media S.A. 2022-04-07 /pmc/articles/PMC9021396/ /pubmed/35464449 http://dx.doi.org/10.3389/fimmu.2022.848571 Text en Copyright © 2022 Bonduelle, Chaudesaigues, Tolazzi, Suleiman, de Bernard, Alves, Nourikyan, Bohec, Baudrin, Katinger, Debré, Scarlatti, Vieillard and Combadière https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Bonduelle, Olivia Chaudesaigues, Chloé Tolazzi, Monica Suleiman, Ehsan de Bernard, Simon Alves, Karine Nourikyan, Julien Bohec, Mylene Baudrin, Laura G. Katinger, Dietmar Debré, Patrice Scarlatti, Gabriella Vieillard, Vincent Combadière, Behazine Dichotomy in Neutralizing Antibody Induction to Peptide-Conjugated Vaccine in Squalene Emulsion Contrast With Aluminum Hydroxide Formulation |
title | Dichotomy in Neutralizing Antibody Induction to Peptide-Conjugated Vaccine in Squalene Emulsion Contrast With Aluminum Hydroxide Formulation |
title_full | Dichotomy in Neutralizing Antibody Induction to Peptide-Conjugated Vaccine in Squalene Emulsion Contrast With Aluminum Hydroxide Formulation |
title_fullStr | Dichotomy in Neutralizing Antibody Induction to Peptide-Conjugated Vaccine in Squalene Emulsion Contrast With Aluminum Hydroxide Formulation |
title_full_unstemmed | Dichotomy in Neutralizing Antibody Induction to Peptide-Conjugated Vaccine in Squalene Emulsion Contrast With Aluminum Hydroxide Formulation |
title_short | Dichotomy in Neutralizing Antibody Induction to Peptide-Conjugated Vaccine in Squalene Emulsion Contrast With Aluminum Hydroxide Formulation |
title_sort | dichotomy in neutralizing antibody induction to peptide-conjugated vaccine in squalene emulsion contrast with aluminum hydroxide formulation |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9021396/ https://www.ncbi.nlm.nih.gov/pubmed/35464449 http://dx.doi.org/10.3389/fimmu.2022.848571 |
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