Cargando…

Prevalence and Characteristics of mcr-1-Producing Escherichia coli in Three Kinds of Poultry in Changsha, China

Colistin is one of the last-line drugs against difficult to treat and multidrug-resistant Gram-negative bacteria. The emergence of mobile colistin resistance gene mcr-1 increased worldwide attention on colistin resistance. mcr-1 is the dominant gene that caused resistance to colistin in chicken-deri...

Descripción completa

Detalles Bibliográficos
Autores principales: Hu, Jufang, Yang, Jie, Chen, Wenxin, Liu, Zhihong, Zhao, Qin, Yang, Hui, Sun, Zhiliang, Chen, Xiaojun, Li, Jiyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9021793/
https://www.ncbi.nlm.nih.gov/pubmed/35464934
http://dx.doi.org/10.3389/fmicb.2022.840520
_version_ 1784689916532228096
author Hu, Jufang
Yang, Jie
Chen, Wenxin
Liu, Zhihong
Zhao, Qin
Yang, Hui
Sun, Zhiliang
Chen, Xiaojun
Li, Jiyun
author_facet Hu, Jufang
Yang, Jie
Chen, Wenxin
Liu, Zhihong
Zhao, Qin
Yang, Hui
Sun, Zhiliang
Chen, Xiaojun
Li, Jiyun
author_sort Hu, Jufang
collection PubMed
description Colistin is one of the last-line drugs against difficult to treat and multidrug-resistant Gram-negative bacteria. The emergence of mobile colistin resistance gene mcr-1 increased worldwide attention on colistin resistance. mcr-1 is the dominant gene that caused resistance to colistin in chicken-derived Escherichia coli (E. coli) in China; it has a broad resistance spectrum and causes multiple drug resistance problems. There are only few studies on mcr-positive E. coli (MCRPEC) from laying ducks and quails in China. Here, the molecular and epidemiological characteristics of MCRPEC from three kinds of poultry farms (laying duck, quail, and broiler) were investigated in Changsha, China. A total of 17 mcr-positive E. coli (MCRPEC) strains were screened in 690 samples from the three kinds of poultry farms. This is the first report on MCRPEC, to our best knowledge, derived from quail. All the MCRPEC strains were resistant to colistin, sulfamethoxazole-trimethoprim, florfenicol, tetracycline, and ciprofloxacin, and mildly resistant to tigecycline, gentamicin, piperacillin/tazobactam, cefotaxime, and ceftiofur. All the strains were sensitive to meropenem and amikacin. By bioinformatics analysis, 17 MCRPEC strains belonging to 11 MLST types were distributed in phylogroups A (58.8%), B1 (23.5%), and phylogroup D (17.6%). mcr-1 was located in IncI2 plasmid with typical plasmid conjugation transfer part, type IV secretory system, and tellurium-resistant protein, increasing transmission capacity to other bacteria. Monitoring of colistin-resistant bacteria in poultry farms should be strengthened.
format Online
Article
Text
id pubmed-9021793
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-90217932022-04-22 Prevalence and Characteristics of mcr-1-Producing Escherichia coli in Three Kinds of Poultry in Changsha, China Hu, Jufang Yang, Jie Chen, Wenxin Liu, Zhihong Zhao, Qin Yang, Hui Sun, Zhiliang Chen, Xiaojun Li, Jiyun Front Microbiol Microbiology Colistin is one of the last-line drugs against difficult to treat and multidrug-resistant Gram-negative bacteria. The emergence of mobile colistin resistance gene mcr-1 increased worldwide attention on colistin resistance. mcr-1 is the dominant gene that caused resistance to colistin in chicken-derived Escherichia coli (E. coli) in China; it has a broad resistance spectrum and causes multiple drug resistance problems. There are only few studies on mcr-positive E. coli (MCRPEC) from laying ducks and quails in China. Here, the molecular and epidemiological characteristics of MCRPEC from three kinds of poultry farms (laying duck, quail, and broiler) were investigated in Changsha, China. A total of 17 mcr-positive E. coli (MCRPEC) strains were screened in 690 samples from the three kinds of poultry farms. This is the first report on MCRPEC, to our best knowledge, derived from quail. All the MCRPEC strains were resistant to colistin, sulfamethoxazole-trimethoprim, florfenicol, tetracycline, and ciprofloxacin, and mildly resistant to tigecycline, gentamicin, piperacillin/tazobactam, cefotaxime, and ceftiofur. All the strains were sensitive to meropenem and amikacin. By bioinformatics analysis, 17 MCRPEC strains belonging to 11 MLST types were distributed in phylogroups A (58.8%), B1 (23.5%), and phylogroup D (17.6%). mcr-1 was located in IncI2 plasmid with typical plasmid conjugation transfer part, type IV secretory system, and tellurium-resistant protein, increasing transmission capacity to other bacteria. Monitoring of colistin-resistant bacteria in poultry farms should be strengthened. Frontiers Media S.A. 2022-04-07 /pmc/articles/PMC9021793/ /pubmed/35464934 http://dx.doi.org/10.3389/fmicb.2022.840520 Text en Copyright © 2022 Hu, Yang, Chen, Liu, Zhao, Yang, Sun, Chen and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Hu, Jufang
Yang, Jie
Chen, Wenxin
Liu, Zhihong
Zhao, Qin
Yang, Hui
Sun, Zhiliang
Chen, Xiaojun
Li, Jiyun
Prevalence and Characteristics of mcr-1-Producing Escherichia coli in Three Kinds of Poultry in Changsha, China
title Prevalence and Characteristics of mcr-1-Producing Escherichia coli in Three Kinds of Poultry in Changsha, China
title_full Prevalence and Characteristics of mcr-1-Producing Escherichia coli in Three Kinds of Poultry in Changsha, China
title_fullStr Prevalence and Characteristics of mcr-1-Producing Escherichia coli in Three Kinds of Poultry in Changsha, China
title_full_unstemmed Prevalence and Characteristics of mcr-1-Producing Escherichia coli in Three Kinds of Poultry in Changsha, China
title_short Prevalence and Characteristics of mcr-1-Producing Escherichia coli in Three Kinds of Poultry in Changsha, China
title_sort prevalence and characteristics of mcr-1-producing escherichia coli in three kinds of poultry in changsha, china
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9021793/
https://www.ncbi.nlm.nih.gov/pubmed/35464934
http://dx.doi.org/10.3389/fmicb.2022.840520
work_keys_str_mv AT hujufang prevalenceandcharacteristicsofmcr1producingescherichiacoliinthreekindsofpoultryinchangshachina
AT yangjie prevalenceandcharacteristicsofmcr1producingescherichiacoliinthreekindsofpoultryinchangshachina
AT chenwenxin prevalenceandcharacteristicsofmcr1producingescherichiacoliinthreekindsofpoultryinchangshachina
AT liuzhihong prevalenceandcharacteristicsofmcr1producingescherichiacoliinthreekindsofpoultryinchangshachina
AT zhaoqin prevalenceandcharacteristicsofmcr1producingescherichiacoliinthreekindsofpoultryinchangshachina
AT yanghui prevalenceandcharacteristicsofmcr1producingescherichiacoliinthreekindsofpoultryinchangshachina
AT sunzhiliang prevalenceandcharacteristicsofmcr1producingescherichiacoliinthreekindsofpoultryinchangshachina
AT chenxiaojun prevalenceandcharacteristicsofmcr1producingescherichiacoliinthreekindsofpoultryinchangshachina
AT lijiyun prevalenceandcharacteristicsofmcr1producingescherichiacoliinthreekindsofpoultryinchangshachina