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Measuring Locomotor Activity and Behavioral Aspects of Rodents Living in the Home-Cage

Automatization and technological advances have led to a larger number of methods and systems to monitor and measure locomotor activity and more specific behavior of a wide variety of animal species in various environmental conditions in laboratory settings. In rodents, the majority of these systems...

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Autores principales: Klein, Christian J. M. I., Budiman, Thomas, Homberg, Judith R., Verma, Dilip, Keijer, Jaap, van Schothorst, Evert M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9021872/
https://www.ncbi.nlm.nih.gov/pubmed/35464142
http://dx.doi.org/10.3389/fnbeh.2022.877323
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author Klein, Christian J. M. I.
Budiman, Thomas
Homberg, Judith R.
Verma, Dilip
Keijer, Jaap
van Schothorst, Evert M.
author_facet Klein, Christian J. M. I.
Budiman, Thomas
Homberg, Judith R.
Verma, Dilip
Keijer, Jaap
van Schothorst, Evert M.
author_sort Klein, Christian J. M. I.
collection PubMed
description Automatization and technological advances have led to a larger number of methods and systems to monitor and measure locomotor activity and more specific behavior of a wide variety of animal species in various environmental conditions in laboratory settings. In rodents, the majority of these systems require the animals to be temporarily taken away from their home-cage into separate observation cage environments which requires manual handling and consequently evokes distress for the animal and may alter behavioral responses. An automated high-throughput approach can overcome this problem. Therefore, this review describes existing automated methods and technologies which enable the measurement of locomotor activity and behavioral aspects of rodents in their most meaningful and stress-free laboratory environment: the home-cage. In line with the Directive 2010/63/EU and the 3R principles (replacement, reduction, refinement), this review furthermore assesses their suitability and potential for group-housed conditions as a refinement strategy, highlighting their current technological and practical limitations. It covers electrical capacitance technology and radio-frequency identification (RFID), which focus mainly on voluntary locomotor activity in both single and multiple rodents, respectively. Infrared beams and force plates expand the detection beyond locomotor activity toward basic behavioral traits but discover their full potential in individually housed rodents only. Despite the great premises of these approaches in terms of behavioral pattern recognition, more sophisticated methods, such as (RFID-assisted) video tracking technology need to be applied to enable the automated analysis of advanced behavioral aspects of individual animals in social housing conditions.
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spelling pubmed-90218722022-04-22 Measuring Locomotor Activity and Behavioral Aspects of Rodents Living in the Home-Cage Klein, Christian J. M. I. Budiman, Thomas Homberg, Judith R. Verma, Dilip Keijer, Jaap van Schothorst, Evert M. Front Behav Neurosci Neuroscience Automatization and technological advances have led to a larger number of methods and systems to monitor and measure locomotor activity and more specific behavior of a wide variety of animal species in various environmental conditions in laboratory settings. In rodents, the majority of these systems require the animals to be temporarily taken away from their home-cage into separate observation cage environments which requires manual handling and consequently evokes distress for the animal and may alter behavioral responses. An automated high-throughput approach can overcome this problem. Therefore, this review describes existing automated methods and technologies which enable the measurement of locomotor activity and behavioral aspects of rodents in their most meaningful and stress-free laboratory environment: the home-cage. In line with the Directive 2010/63/EU and the 3R principles (replacement, reduction, refinement), this review furthermore assesses their suitability and potential for group-housed conditions as a refinement strategy, highlighting their current technological and practical limitations. It covers electrical capacitance technology and radio-frequency identification (RFID), which focus mainly on voluntary locomotor activity in both single and multiple rodents, respectively. Infrared beams and force plates expand the detection beyond locomotor activity toward basic behavioral traits but discover their full potential in individually housed rodents only. Despite the great premises of these approaches in terms of behavioral pattern recognition, more sophisticated methods, such as (RFID-assisted) video tracking technology need to be applied to enable the automated analysis of advanced behavioral aspects of individual animals in social housing conditions. Frontiers Media S.A. 2022-04-07 /pmc/articles/PMC9021872/ /pubmed/35464142 http://dx.doi.org/10.3389/fnbeh.2022.877323 Text en Copyright © 2022 Klein, Budiman, Homberg, Verma, Keijer and van Schothorst. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Klein, Christian J. M. I.
Budiman, Thomas
Homberg, Judith R.
Verma, Dilip
Keijer, Jaap
van Schothorst, Evert M.
Measuring Locomotor Activity and Behavioral Aspects of Rodents Living in the Home-Cage
title Measuring Locomotor Activity and Behavioral Aspects of Rodents Living in the Home-Cage
title_full Measuring Locomotor Activity and Behavioral Aspects of Rodents Living in the Home-Cage
title_fullStr Measuring Locomotor Activity and Behavioral Aspects of Rodents Living in the Home-Cage
title_full_unstemmed Measuring Locomotor Activity and Behavioral Aspects of Rodents Living in the Home-Cage
title_short Measuring Locomotor Activity and Behavioral Aspects of Rodents Living in the Home-Cage
title_sort measuring locomotor activity and behavioral aspects of rodents living in the home-cage
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9021872/
https://www.ncbi.nlm.nih.gov/pubmed/35464142
http://dx.doi.org/10.3389/fnbeh.2022.877323
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