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Characterization of the Phase-Variable Autotransporter Lav Reveals a Role in Host Cell Adherence and Biofilm Formation in Nontypeable Haemophilus influenzae
Lav is an autotransporter protein found in pathogenic Haemophilus and Neisseria species. Lav in nontypeable Haemophilus influenzae (NTHi) is phase-variable: the gene reversibly switches ON-OFF via changes in length of a locus-located GCAA((n)) simple DNA sequence repeat tract. The expression status...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Microbiology
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9022572/ https://www.ncbi.nlm.nih.gov/pubmed/35258316 http://dx.doi.org/10.1128/iai.00565-21 |
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author | Phillips, Zachary N. Garai, Preeti Tram, Greg Martin, Gael Van Den Bergh, Annelies Husna, Asma-Ul Staples, Megan Grimwood, Keith Jennison, Amy V. Guillon, Patrice von Itzstein, Mark Jennings, Michael P. Brockman, Kenneth L. Atack, John M. |
author_facet | Phillips, Zachary N. Garai, Preeti Tram, Greg Martin, Gael Van Den Bergh, Annelies Husna, Asma-Ul Staples, Megan Grimwood, Keith Jennison, Amy V. Guillon, Patrice von Itzstein, Mark Jennings, Michael P. Brockman, Kenneth L. Atack, John M. |
author_sort | Phillips, Zachary N. |
collection | PubMed |
description | Lav is an autotransporter protein found in pathogenic Haemophilus and Neisseria species. Lav in nontypeable Haemophilus influenzae (NTHi) is phase-variable: the gene reversibly switches ON-OFF via changes in length of a locus-located GCAA((n)) simple DNA sequence repeat tract. The expression status of lav was examined in carriage and invasive collections of NTHi, where it was predominantly not expressed (OFF). Phenotypic study showed lav expression (ON) results in increased adherence to human lung cells and denser biofilm formation. A survey of Haemophilus species genome sequences showed lav is present in ∼60% of NTHi strains, but lav is not present in most typeable H. influenzae strains. Sequence analysis revealed a total of five distinct variants of the Lav passenger domain present in Haemophilus spp., with these five variants showing a distinct lineage distribution. Determining the role of Lav in NTHi will help understand the role of this protein during distinct pathologies. |
format | Online Article Text |
id | pubmed-9022572 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Society for Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-90225722022-04-22 Characterization of the Phase-Variable Autotransporter Lav Reveals a Role in Host Cell Adherence and Biofilm Formation in Nontypeable Haemophilus influenzae Phillips, Zachary N. Garai, Preeti Tram, Greg Martin, Gael Van Den Bergh, Annelies Husna, Asma-Ul Staples, Megan Grimwood, Keith Jennison, Amy V. Guillon, Patrice von Itzstein, Mark Jennings, Michael P. Brockman, Kenneth L. Atack, John M. Infect Immun Molecular Pathogenesis Lav is an autotransporter protein found in pathogenic Haemophilus and Neisseria species. Lav in nontypeable Haemophilus influenzae (NTHi) is phase-variable: the gene reversibly switches ON-OFF via changes in length of a locus-located GCAA((n)) simple DNA sequence repeat tract. The expression status of lav was examined in carriage and invasive collections of NTHi, where it was predominantly not expressed (OFF). Phenotypic study showed lav expression (ON) results in increased adherence to human lung cells and denser biofilm formation. A survey of Haemophilus species genome sequences showed lav is present in ∼60% of NTHi strains, but lav is not present in most typeable H. influenzae strains. Sequence analysis revealed a total of five distinct variants of the Lav passenger domain present in Haemophilus spp., with these five variants showing a distinct lineage distribution. Determining the role of Lav in NTHi will help understand the role of this protein during distinct pathologies. American Society for Microbiology 2022-03-08 /pmc/articles/PMC9022572/ /pubmed/35258316 http://dx.doi.org/10.1128/iai.00565-21 Text en Copyright © 2022 Phillips et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Molecular Pathogenesis Phillips, Zachary N. Garai, Preeti Tram, Greg Martin, Gael Van Den Bergh, Annelies Husna, Asma-Ul Staples, Megan Grimwood, Keith Jennison, Amy V. Guillon, Patrice von Itzstein, Mark Jennings, Michael P. Brockman, Kenneth L. Atack, John M. Characterization of the Phase-Variable Autotransporter Lav Reveals a Role in Host Cell Adherence and Biofilm Formation in Nontypeable Haemophilus influenzae |
title | Characterization of the Phase-Variable Autotransporter Lav Reveals a Role in Host Cell Adherence and Biofilm Formation in Nontypeable Haemophilus influenzae |
title_full | Characterization of the Phase-Variable Autotransporter Lav Reveals a Role in Host Cell Adherence and Biofilm Formation in Nontypeable Haemophilus influenzae |
title_fullStr | Characterization of the Phase-Variable Autotransporter Lav Reveals a Role in Host Cell Adherence and Biofilm Formation in Nontypeable Haemophilus influenzae |
title_full_unstemmed | Characterization of the Phase-Variable Autotransporter Lav Reveals a Role in Host Cell Adherence and Biofilm Formation in Nontypeable Haemophilus influenzae |
title_short | Characterization of the Phase-Variable Autotransporter Lav Reveals a Role in Host Cell Adherence and Biofilm Formation in Nontypeable Haemophilus influenzae |
title_sort | characterization of the phase-variable autotransporter lav reveals a role in host cell adherence and biofilm formation in nontypeable haemophilus influenzae |
topic | Molecular Pathogenesis |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9022572/ https://www.ncbi.nlm.nih.gov/pubmed/35258316 http://dx.doi.org/10.1128/iai.00565-21 |
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