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Packaging contests between viral RNA molecules and kinetic selectivity
The paper presents a statistical-mechanics model for the kinetic selection of viral RNA molecules by packaging signals during the nucleation stage of the assembly of small RNA viruses. The effects of the RNA secondary structure and folding geometry of the packaging signals on the assembly activation...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9022832/ https://www.ncbi.nlm.nih.gov/pubmed/35363785 http://dx.doi.org/10.1371/journal.pcbi.1009913 |
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author | Mizrahi, Inbal Bruinsma, Robijn Rudnick, Joseph |
author_facet | Mizrahi, Inbal Bruinsma, Robijn Rudnick, Joseph |
author_sort | Mizrahi, Inbal |
collection | PubMed |
description | The paper presents a statistical-mechanics model for the kinetic selection of viral RNA molecules by packaging signals during the nucleation stage of the assembly of small RNA viruses. The effects of the RNA secondary structure and folding geometry of the packaging signals on the assembly activation energy barrier are encoded by a pair of characteristics: the wrapping number and the maximum ladder distance. Kinetic selection is found to be optimal when assembly takes place under conditions of supersaturation and also when the concentration ratio of capsid protein and viral RNA concentrations equals the stoichiometric ratio of assembled viral particles. As a function of the height of the activation energy barrier, there is a form of order-disorder transition such that for sufficiently low activation energy barriers, kinetic selectivity is erased by entropic effects associated with the number of assembly pathways. |
format | Online Article Text |
id | pubmed-9022832 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-90228322022-04-22 Packaging contests between viral RNA molecules and kinetic selectivity Mizrahi, Inbal Bruinsma, Robijn Rudnick, Joseph PLoS Comput Biol Research Article The paper presents a statistical-mechanics model for the kinetic selection of viral RNA molecules by packaging signals during the nucleation stage of the assembly of small RNA viruses. The effects of the RNA secondary structure and folding geometry of the packaging signals on the assembly activation energy barrier are encoded by a pair of characteristics: the wrapping number and the maximum ladder distance. Kinetic selection is found to be optimal when assembly takes place under conditions of supersaturation and also when the concentration ratio of capsid protein and viral RNA concentrations equals the stoichiometric ratio of assembled viral particles. As a function of the height of the activation energy barrier, there is a form of order-disorder transition such that for sufficiently low activation energy barriers, kinetic selectivity is erased by entropic effects associated with the number of assembly pathways. Public Library of Science 2022-04-01 /pmc/articles/PMC9022832/ /pubmed/35363785 http://dx.doi.org/10.1371/journal.pcbi.1009913 Text en © 2022 Mizrahi et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Mizrahi, Inbal Bruinsma, Robijn Rudnick, Joseph Packaging contests between viral RNA molecules and kinetic selectivity |
title | Packaging contests between viral RNA molecules and kinetic selectivity |
title_full | Packaging contests between viral RNA molecules and kinetic selectivity |
title_fullStr | Packaging contests between viral RNA molecules and kinetic selectivity |
title_full_unstemmed | Packaging contests between viral RNA molecules and kinetic selectivity |
title_short | Packaging contests between viral RNA molecules and kinetic selectivity |
title_sort | packaging contests between viral rna molecules and kinetic selectivity |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9022832/ https://www.ncbi.nlm.nih.gov/pubmed/35363785 http://dx.doi.org/10.1371/journal.pcbi.1009913 |
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