Cargando…

Partial recovery of voiding function in female mice following repeated psychological stress exposure

Psychological stress causes bladder dysfunction in humans and in rodent models, with increased urinary frequency and altered contractile responses evident following repeated environmental stress exposure. However, whether these changes persist after removal of the stressor is unknown, and the aim of...

Descripción completa

Detalles Bibliográficos
Autores principales: West, Eliza G., McDermott, Catherine, Chess-Williams, Russ, Sellers, Donna J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9022836/
https://www.ncbi.nlm.nih.gov/pubmed/35446874
http://dx.doi.org/10.1371/journal.pone.0266458
_version_ 1784690182561202176
author West, Eliza G.
McDermott, Catherine
Chess-Williams, Russ
Sellers, Donna J.
author_facet West, Eliza G.
McDermott, Catherine
Chess-Williams, Russ
Sellers, Donna J.
author_sort West, Eliza G.
collection PubMed
description Psychological stress causes bladder dysfunction in humans and in rodent models, with increased urinary frequency and altered contractile responses evident following repeated environmental stress exposure. However, whether these changes persist after removal of the stressor is unknown, and the aim of this study was to determine if stress-induced changes in voiding behaviour and bladder function recover following removal of the stressor. Adult female mice were allocated to three groups: Unstressed, Stressed or Stressed + Recovery. Animals in the stressed groups were exposed to water avoidance stress for 1h/day for 10-days, with unstressed animals age-matched and housed under normal conditions. For recovery studies, animals were housed without stress exposure for an additional 10-days. Voiding behaviour was assessed periodically and animals sacrificed on day 10 (Unstressed and Stressed) or day 20 (Unstressed and Stressed + Recovery). Isolated whole bladder studies were used to assess compliance, urothelial mediator release and contractile responses. Exposure to stress increased plasma corticosterone levels almost three-fold (P<0.05) but this returned to baseline during the recovery period. Contractile responses of the bladder to carbachol and KCl were also increased following stress, and again fully recovered after a 10-day stress-free period. In contrast, stress increased urinary frequency four-fold (P<0.001), but this did not return fully to baseline during the recovery period. Bladder compliance was unchanged by stress; however, it was increased in the stressed + recovery group (P<0.05). Thus, following a stress-free period there is partial recovery of voiding behaviour, with an increase in bladder compliance possibly contributing to the compensatory mechanisms.
format Online
Article
Text
id pubmed-9022836
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-90228362022-04-22 Partial recovery of voiding function in female mice following repeated psychological stress exposure West, Eliza G. McDermott, Catherine Chess-Williams, Russ Sellers, Donna J. PLoS One Research Article Psychological stress causes bladder dysfunction in humans and in rodent models, with increased urinary frequency and altered contractile responses evident following repeated environmental stress exposure. However, whether these changes persist after removal of the stressor is unknown, and the aim of this study was to determine if stress-induced changes in voiding behaviour and bladder function recover following removal of the stressor. Adult female mice were allocated to three groups: Unstressed, Stressed or Stressed + Recovery. Animals in the stressed groups were exposed to water avoidance stress for 1h/day for 10-days, with unstressed animals age-matched and housed under normal conditions. For recovery studies, animals were housed without stress exposure for an additional 10-days. Voiding behaviour was assessed periodically and animals sacrificed on day 10 (Unstressed and Stressed) or day 20 (Unstressed and Stressed + Recovery). Isolated whole bladder studies were used to assess compliance, urothelial mediator release and contractile responses. Exposure to stress increased plasma corticosterone levels almost three-fold (P<0.05) but this returned to baseline during the recovery period. Contractile responses of the bladder to carbachol and KCl were also increased following stress, and again fully recovered after a 10-day stress-free period. In contrast, stress increased urinary frequency four-fold (P<0.001), but this did not return fully to baseline during the recovery period. Bladder compliance was unchanged by stress; however, it was increased in the stressed + recovery group (P<0.05). Thus, following a stress-free period there is partial recovery of voiding behaviour, with an increase in bladder compliance possibly contributing to the compensatory mechanisms. Public Library of Science 2022-04-21 /pmc/articles/PMC9022836/ /pubmed/35446874 http://dx.doi.org/10.1371/journal.pone.0266458 Text en © 2022 West et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
West, Eliza G.
McDermott, Catherine
Chess-Williams, Russ
Sellers, Donna J.
Partial recovery of voiding function in female mice following repeated psychological stress exposure
title Partial recovery of voiding function in female mice following repeated psychological stress exposure
title_full Partial recovery of voiding function in female mice following repeated psychological stress exposure
title_fullStr Partial recovery of voiding function in female mice following repeated psychological stress exposure
title_full_unstemmed Partial recovery of voiding function in female mice following repeated psychological stress exposure
title_short Partial recovery of voiding function in female mice following repeated psychological stress exposure
title_sort partial recovery of voiding function in female mice following repeated psychological stress exposure
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9022836/
https://www.ncbi.nlm.nih.gov/pubmed/35446874
http://dx.doi.org/10.1371/journal.pone.0266458
work_keys_str_mv AT westelizag partialrecoveryofvoidingfunctioninfemalemicefollowingrepeatedpsychologicalstressexposure
AT mcdermottcatherine partialrecoveryofvoidingfunctioninfemalemicefollowingrepeatedpsychologicalstressexposure
AT chesswilliamsruss partialrecoveryofvoidingfunctioninfemalemicefollowingrepeatedpsychologicalstressexposure
AT sellersdonnaj partialrecoveryofvoidingfunctioninfemalemicefollowingrepeatedpsychologicalstressexposure