Cargando…

A real-time PCR method to genotype mutant mouse models with altered affinity for cardiotonic steroids on the Na,K-ATPase

The highly conserved, cardiotonic steroid binding site (also termed ouabain binding site) on the primary α subunit of Na,K-ATPase plays a receptor signaling role in a range of vital cell processes and is a therapeutic target for human disease. Mouse lines with altered affinity for cardiotonic steroi...

Descripción completa

Detalles Bibliográficos
Autores principales:	Chomczynski, Peter W., Vires, Kianna M., Rymaszewski, Michal, Heiny, Judith A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2022
Materias:	Lab Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9022855/ https://www.ncbi.nlm.nih.gov/pubmed/35446892 http://dx.doi.org/10.1371/journal.pone.0267348

Ejemplares similares

Na/K-ATPase Signaling and Cardiac Pre/Postconditioning with Cardiotonic Steroids
por: Marck, Pauline V., et al.
Publicado: (2018)

Na(+),K(+)-ATPase and Cardiotonic Steroids in Models of Dopaminergic System Pathologies
por: Markina, Alisa A., et al.
Publicado: (2023)

Na,K-ATPase as a target for endogenous cardiotonic steroids: What's the evidence?
por: Orlov, Sergei N., et al.
Publicado: (2020)

Depth of the Steroid Core Location Determines the Mode of Na,K-ATPase Inhibition by Cardiotonic Steroids
por: Tverskoi, Artem M., et al.
Publicado: (2021)

Rapid processing of SARS-CoV-2 containing specimens for direct RT-PCR
por: Chomczynski, Piotr, et al.
Publicado: (2021)

Cardiac Oxidative Signaling and Physiological Hypertrophy in the Na/K-ATPase α1(s/s)α2(s/s) Mouse Model of High Affinity for Cardiotonic Steroids
por: Marck, Pauline V., et al.
Publicado: (2021)

Novel stereoselective bufadienolides reveal new insights into the requirements for Na(+), K(+)-ATPase inhibition by cardiotonic steroids
por: Tang, Hong-Jin, et al.
Publicado: (2016)

Cryoelectron microscopy of Na(+),K(+)-ATPase in the two E2P states with and without cardiotonic steroids
por: Kanai, Ryuta, et al.
Publicado: (2022)

Cardiotonic Steroids and the Sodium Trade Balance: New Insights into Trade-Off Mechanisms Mediated by the Na(+)/K(+)-ATPase
por: Khalaf, Fatimah K., et al.
Publicado: (2018)

Identification of the NA(+)/K(+)-ATPase α-Isoforms in Six Species of Poison Dart Frogs and their Sensitivity to Cardiotonic Steroids
por: Medina-Ortiz, Katherine, et al.
Publicado: (2023)

Telocinobufagin, a Novel Cardiotonic Steroid, Promotes Renal Fibrosis via Na(+)/K(+)-ATPase Profibrotic Signaling Pathways
por: Kennedy, David J., et al.
Publicado: (2018)

Epithelial and Endothelial Adhesion of Immune Cells Is Enhanced by Cardiotonic Steroid Signaling Through Na(+)/K(+)‐ATPase‐α‐1
por: Khalaf, Fatimah K., et al.
Publicado: (2020)

Cardiotonic Steroids as Modulators of Neuroinflammation
por: Orellana, Ana Maria, et al.
Publicado: (2016)

Na(+)(i),K(+)(i)-Dependent and -Independent Signaling Triggered by Cardiotonic Steroids: Facts and Artifacts
por: Orlov, Sergei N., et al.
Publicado: (2017)

Endogenous cardiotonic steroids and cardiovascular disease, where to next?
por: Pavlovic, Davor
Publicado: (2020)

Research Progress in Pharmacological Activities and Applications of Cardiotonic Steroids
por: Ren, Junwei, et al.
Publicado: (2022)

K(+) and Rb(+) Affinities of the Na,K-ATPase α(1) and α(2) Isozymes: An Application of ICP-MS for Quantification of Na(+) Pump Kinetics in Myofibers
por: Hakimjavadi, Hesamedin, et al.
Publicado: (2018)

Time- and dose dependent actions of cardiotonic steroids on transcriptome and intracellular content of Na(+) and K(+): a comparative analysis
por: Klimanova, Elizaveta A., et al.
Publicado: (2017)

Preeclampsia: Cardiotonic Steroids, Fibrosis, Fli1 and Hint to Carcinogenesis
por: Agalakova, Natalia I., et al.
Publicado: (2021)

Probing the stereoselectivity of OleD-catalyzed glycosylation of cardiotonic steroids
por: Zhu, Xue-Lin, et al.
Publicado: (2018)

The Na+/K+ ATPase Inhibitor Ouabain Attenuates Stemness and Chemoresistance of Osteosarcoma Cells
por: Guo, Weixiong, et al.
Publicado: (2019)

Much More than a Cardiotonic Steroid: Modulation of Inflammation by Ouabain
por: Cavalcante-Silva, Luiz H. A., et al.
Publicado: (2017)

Corrigendum: Much More than a Cardiotonic Steroid: Modulation of Inflammation by Ouabain
por: Cavalcante-Silva, Luiz H. A., et al.
Publicado: (2018)

Na,K-ATPase α2 activity in mammalian skeletal muscle T-tubules is acutely stimulated by extracellular K(+)
por: DiFranco, Marino, et al.
Publicado: (2015)

β subunit affects Na(+) and K(+) affinities of Na(+)/K(+)-ATPase: Na(+) and K(+) affinities of a hybrid Na(+)/K(+)-ATPase composed of insect α and mammalian β subunits
por: Homareda, Haruo, et al.
Publicado: (2022)

Cardiotonic Steroids—A Possible Link Between High-Salt Diet and Organ Damage
por: Paczula, Aneta, et al.
Publicado: (2019)

Effect of Cardiotonic Steroid Marinobufagenin on Vascular Remodeling and Cognitive Impairment in Young Dahl-S Rats
por: Grigorova, Yulia N., et al.
Publicado: (2022)

Structure-Activity Relationships of Several Cardiotonic Steroids with Respect to Inhibition of Ion Transport in Frog Muscle
por: Abeles, A. L.
Publicado: (1969)

Endogenous Mammalian Cardiotonic Steroids—A New Cardiovascular Risk Factor?—A Mini-Review
por: Słabiak-Błaż, Natalia, et al.
Publicado: (2021)

Broad Spectrum Antiviral Properties of Cardiotonic Steroids Used as Potential Therapeutics for Emerging Coronavirus Infections
por: Jin, Young-Hee, et al.
Publicado: (2021)

Chemistry and the Potential Antiviral, Anticancer, and Anti-Inflammatory Activities of Cardiotonic Steroids Derived from Toads †
por: El-Seedi, Hesham R., et al.
Publicado: (2022)

Ca(2+)-Driven Selectivity of the Effect of the Cardiotonic Steroid Marinobufagenin on Rabbit Sinoatrial Node Function
por: Segal, Sofia, et al.
Publicado: (2023)

Isoform-Specific Na,K-ATPase Alterations Precede Disuse-Induced Atrophy of Rat Soleus Muscle
por: Kravtsova, Violetta V., et al.
Publicado: (2015)

The Cardiotonic Steroid Marinobufagenin Is a Predictor of Increased Left Ventricular Mass in Obesity: The African-PREDICT Study
por: Strauss-Kruger, Michél, et al.
Publicado: (2020)

The endogenous cardiotonic steroid Marinobufagenin and decline in estimated glomerular filtration rate at follow-up in patients with arterial hypertension
por: Keppel, Martin H., et al.
Publicado: (2019)

Insect Collections as an Untapped Source of Bioactive Compounds—Fireflies (Coleoptera: Lampyridae) and Cardiotonic Steroids as a Proof of Concept
por: Berger, Andreas, et al.
Publicado: (2021)

Cardiotonic Drugs from the Avicenna’s Point of View
por: GHORBANI, Fariba, et al.
Publicado: (2015)

Chronic Nicotine Modifies Skeletal Muscle Na,K-ATPase Activity through Its Interaction with the Nicotinic Acetylcholine Receptor and Phospholemman
por: Chibalin, Alexander V., et al.
Publicado: (2012)

Development of a real-time PCR assay to detect the single nucleotide polymorphism causing Warmblood Fragile Foal Syndrome
por: Flanagan, Sharon, et al.
Publicado: (2021)

Surface Plasmon Resonance Biosensor Method for Palytoxin Detection Based on Na(+),K(+)-ATPase Affinity
por: Alfonso, Amparo, et al.
Publicado: (2013)

Cannot write session to /tmp/vufind_sessions/sess_c9n9nfuqfja7nlri5ojdg5j54f