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Shaping axial identity during human pluripotent stem cell differentiation to neural crest cells

The neural crest (NC) is a multipotent cell population which can give rise to a vast array of derivatives including neurons and glia of the peripheral nervous system, cartilage, cardiac smooth muscle, melanocytes and sympathoadrenal cells. An attractive strategy to model human NC development and ass...

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Detalles Bibliográficos
Autores principales: Cooper, Fay, Tsakiridis, Anestis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9022984/
https://www.ncbi.nlm.nih.gov/pubmed/35015077
http://dx.doi.org/10.1042/BST20211152
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author Cooper, Fay
Tsakiridis, Anestis
author_facet Cooper, Fay
Tsakiridis, Anestis
author_sort Cooper, Fay
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description The neural crest (NC) is a multipotent cell population which can give rise to a vast array of derivatives including neurons and glia of the peripheral nervous system, cartilage, cardiac smooth muscle, melanocytes and sympathoadrenal cells. An attractive strategy to model human NC development and associated birth defects as well as produce clinically relevant cell populations for regenerative medicine applications involves the in vitro generation of NC from human pluripotent stem cells (hPSCs). However, in vivo, the potential of NC cells to generate distinct cell types is determined by their position along the anteroposterior (A–P) axis and, therefore the axial identity of hPSC-derived NC cells is an important aspect to consider. Recent advances in understanding the developmental origins of NC and the signalling pathways involved in its specification have aided the in vitro generation of human NC cells which are representative of various A–P positions. Here, we explore recent advances in methodologies of in vitro NC specification and axis patterning using hPSCs.
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spelling pubmed-90229842022-05-03 Shaping axial identity during human pluripotent stem cell differentiation to neural crest cells Cooper, Fay Tsakiridis, Anestis Biochem Soc Trans Review Articles The neural crest (NC) is a multipotent cell population which can give rise to a vast array of derivatives including neurons and glia of the peripheral nervous system, cartilage, cardiac smooth muscle, melanocytes and sympathoadrenal cells. An attractive strategy to model human NC development and associated birth defects as well as produce clinically relevant cell populations for regenerative medicine applications involves the in vitro generation of NC from human pluripotent stem cells (hPSCs). However, in vivo, the potential of NC cells to generate distinct cell types is determined by their position along the anteroposterior (A–P) axis and, therefore the axial identity of hPSC-derived NC cells is an important aspect to consider. Recent advances in understanding the developmental origins of NC and the signalling pathways involved in its specification have aided the in vitro generation of human NC cells which are representative of various A–P positions. Here, we explore recent advances in methodologies of in vitro NC specification and axis patterning using hPSCs. Portland Press Ltd. 2022-02-28 2021-01-11 /pmc/articles/PMC9022984/ /pubmed/35015077 http://dx.doi.org/10.1042/BST20211152 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Review Articles
Cooper, Fay
Tsakiridis, Anestis
Shaping axial identity during human pluripotent stem cell differentiation to neural crest cells
title Shaping axial identity during human pluripotent stem cell differentiation to neural crest cells
title_full Shaping axial identity during human pluripotent stem cell differentiation to neural crest cells
title_fullStr Shaping axial identity during human pluripotent stem cell differentiation to neural crest cells
title_full_unstemmed Shaping axial identity during human pluripotent stem cell differentiation to neural crest cells
title_short Shaping axial identity during human pluripotent stem cell differentiation to neural crest cells
title_sort shaping axial identity during human pluripotent stem cell differentiation to neural crest cells
topic Review Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9022984/
https://www.ncbi.nlm.nih.gov/pubmed/35015077
http://dx.doi.org/10.1042/BST20211152
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