Performance of the QIAprep&amp Viral RNA UM Kit assay (Qiagen), an automatable method for RT-qPCR detection of SARS-CoV-2 without RNA extraction

We evaluated the performance of the QIAprep&amp Viral RNA UM Kit (Qiagen) for SARS-CoV-2 detection. It displayed specificity and sensitivity required for SARS-CoV-2 RNA detection from swab transport media without RNA extraction. This method identifies accurately patients at risk of transmission...

Descripción completa

Detalles Bibliográficos
Autores principales: Fenaux, Honorine, Limam, Lamia, Soutiere, Marie-Pierre, Veillet, Frédéric, Escuret, Vanessa, Roque-Afonso, Anne-Marie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Author(s). Published by Elsevier Inc. 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9023082/
https://www.ncbi.nlm.nih.gov/pubmed/35605560
http://dx.doi.org/10.1016/j.diagmicrobio.2022.115700
_version_ 1784690256222617600
author Fenaux, Honorine
Limam, Lamia
Soutiere, Marie-Pierre
Veillet, Frédéric
Escuret, Vanessa
Roque-Afonso, Anne-Marie
author_facet Fenaux, Honorine
Limam, Lamia
Soutiere, Marie-Pierre
Veillet, Frédéric
Escuret, Vanessa
Roque-Afonso, Anne-Marie
author_sort Fenaux, Honorine
collection PubMed
description We evaluated the performance of the QIAprep&amp Viral RNA UM Kit (Qiagen) for SARS-CoV-2 detection. It displayed specificity and sensitivity required for SARS-CoV-2 RNA detection from swab transport media without RNA extraction. This method identifies accurately patients at risk of transmission while saving time and cost of extraction.
format Online
Article
Text
id pubmed-9023082
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher The Author(s). Published by Elsevier Inc.
record_format MEDLINE/PubMed
spelling pubmed-90230822022-04-22 Performance of the QIAprep&amp Viral RNA UM Kit assay (Qiagen), an automatable method for RT-qPCR detection of SARS-CoV-2 without RNA extraction Fenaux, Honorine Limam, Lamia Soutiere, Marie-Pierre Veillet, Frédéric Escuret, Vanessa Roque-Afonso, Anne-Marie Diagn Microbiol Infect Dis Article We evaluated the performance of the QIAprep&amp Viral RNA UM Kit (Qiagen) for SARS-CoV-2 detection. It displayed specificity and sensitivity required for SARS-CoV-2 RNA detection from swab transport media without RNA extraction. This method identifies accurately patients at risk of transmission while saving time and cost of extraction. The Author(s). Published by Elsevier Inc. 2022-07 2022-04-21 /pmc/articles/PMC9023082/ /pubmed/35605560 http://dx.doi.org/10.1016/j.diagmicrobio.2022.115700 Text en © 2022 The Author(s) Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Fenaux, Honorine
Limam, Lamia
Soutiere, Marie-Pierre
Veillet, Frédéric
Escuret, Vanessa
Roque-Afonso, Anne-Marie
Performance of the QIAprep&amp Viral RNA UM Kit assay (Qiagen), an automatable method for RT-qPCR detection of SARS-CoV-2 without RNA extraction
title Performance of the QIAprep&amp Viral RNA UM Kit assay (Qiagen), an automatable method for RT-qPCR detection of SARS-CoV-2 without RNA extraction
title_full Performance of the QIAprep&amp Viral RNA UM Kit assay (Qiagen), an automatable method for RT-qPCR detection of SARS-CoV-2 without RNA extraction
title_fullStr Performance of the QIAprep&amp Viral RNA UM Kit assay (Qiagen), an automatable method for RT-qPCR detection of SARS-CoV-2 without RNA extraction
title_full_unstemmed Performance of the QIAprep&amp Viral RNA UM Kit assay (Qiagen), an automatable method for RT-qPCR detection of SARS-CoV-2 without RNA extraction
title_short Performance of the QIAprep&amp Viral RNA UM Kit assay (Qiagen), an automatable method for RT-qPCR detection of SARS-CoV-2 without RNA extraction
title_sort performance of the qiaprep&amp viral rna um kit assay (qiagen), an automatable method for rt-qpcr detection of sars-cov-2 without rna extraction
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9023082/
https://www.ncbi.nlm.nih.gov/pubmed/35605560
http://dx.doi.org/10.1016/j.diagmicrobio.2022.115700
work_keys_str_mv AT fenauxhonorine performanceoftheqiaprepampviralrnaumkitassayqiagenanautomatablemethodforrtqpcrdetectionofsarscov2withoutrnaextraction
AT limamlamia performanceoftheqiaprepampviralrnaumkitassayqiagenanautomatablemethodforrtqpcrdetectionofsarscov2withoutrnaextraction
AT soutieremariepierre performanceoftheqiaprepampviralrnaumkitassayqiagenanautomatablemethodforrtqpcrdetectionofsarscov2withoutrnaextraction
AT veilletfrederic performanceoftheqiaprepampviralrnaumkitassayqiagenanautomatablemethodforrtqpcrdetectionofsarscov2withoutrnaextraction
AT escuretvanessa performanceoftheqiaprepampviralrnaumkitassayqiagenanautomatablemethodforrtqpcrdetectionofsarscov2withoutrnaextraction
AT roqueafonsoannemarie performanceoftheqiaprepampviralrnaumkitassayqiagenanautomatablemethodforrtqpcrdetectionofsarscov2withoutrnaextraction

Ejemplares similares