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Oxidative stress-CBP axis modulates MOB1 acetylation and activates the Hippo signaling pathway

Reactive oxygen species (ROS) are constantly produced in cells, an excess of which causes oxidative stress. ROS has been linked to regulation of the Hippo pathway; however, the underlying detailed mechanisms remain unclear. Here, we report that MOB1, a substrate of MST1/2 and co-activator of LATS1/2...

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Autores principales: Jin, Jiaqi, Zhang, Lei, Li, Xueying, Xu, Weizhi, Yang, Siyuan, Song, Jiagui, Zhang, Wenhao, Zhan, Jun, Luo, Jianyuan, Zhang, Hongquan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9023286/
https://www.ncbi.nlm.nih.gov/pubmed/35349706
http://dx.doi.org/10.1093/nar/gkac189
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author Jin, Jiaqi
Zhang, Lei
Li, Xueying
Xu, Weizhi
Yang, Siyuan
Song, Jiagui
Zhang, Wenhao
Zhan, Jun
Luo, Jianyuan
Zhang, Hongquan
author_facet Jin, Jiaqi
Zhang, Lei
Li, Xueying
Xu, Weizhi
Yang, Siyuan
Song, Jiagui
Zhang, Wenhao
Zhan, Jun
Luo, Jianyuan
Zhang, Hongquan
author_sort Jin, Jiaqi
collection PubMed
description Reactive oxygen species (ROS) are constantly produced in cells, an excess of which causes oxidative stress. ROS has been linked to regulation of the Hippo pathway; however, the underlying detailed mechanisms remain unclear. Here, we report that MOB1, a substrate of MST1/2 and co-activator of LATS1/2 in the canonical Hippo pathway, interacts with and is acetylated at lysine 11 by acetyltransferase CBP and deacetylated by HDAC6. MOB1-K11 acetylation stabilizes itself by reducing its binding capacity with E3 ligase Praja2 and subsequent ubiquitination. MOB1-K11 acetylation increases its phosphorylation and activates LATS1. Importantly, upstream oxidative stress signals promote MOB1 acetylation by suppressing CBP degradation, independent of MST1/2 kinase activity and HDAC6 deacetylation effect, thereby linking oxidative stress to activation of the Hippo pathway. Functionally, the acetylation-deficient mutant MOB1-K11R promotes lung cancer cell proliferation, migration and invasion in vitro and accelerates tumor growth in vivo, compared to the wild-type MOB1. Clinically, acetylated MOB1 corresponds to better prediction of overall survival in patients with non-small cell lung cancer. Therefore, as demonstrated, an oxidative stress-CBP regulatory axis controls MOB1-K11 acetylation and activates LATS1, thereby activating the Hippo pathway and suppressing YAP/TAZ nuclear translocation and tumor progression.
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spelling pubmed-90232862022-04-22 Oxidative stress-CBP axis modulates MOB1 acetylation and activates the Hippo signaling pathway Jin, Jiaqi Zhang, Lei Li, Xueying Xu, Weizhi Yang, Siyuan Song, Jiagui Zhang, Wenhao Zhan, Jun Luo, Jianyuan Zhang, Hongquan Nucleic Acids Res Gene regulation, Chromatin and Epigenetics Reactive oxygen species (ROS) are constantly produced in cells, an excess of which causes oxidative stress. ROS has been linked to regulation of the Hippo pathway; however, the underlying detailed mechanisms remain unclear. Here, we report that MOB1, a substrate of MST1/2 and co-activator of LATS1/2 in the canonical Hippo pathway, interacts with and is acetylated at lysine 11 by acetyltransferase CBP and deacetylated by HDAC6. MOB1-K11 acetylation stabilizes itself by reducing its binding capacity with E3 ligase Praja2 and subsequent ubiquitination. MOB1-K11 acetylation increases its phosphorylation and activates LATS1. Importantly, upstream oxidative stress signals promote MOB1 acetylation by suppressing CBP degradation, independent of MST1/2 kinase activity and HDAC6 deacetylation effect, thereby linking oxidative stress to activation of the Hippo pathway. Functionally, the acetylation-deficient mutant MOB1-K11R promotes lung cancer cell proliferation, migration and invasion in vitro and accelerates tumor growth in vivo, compared to the wild-type MOB1. Clinically, acetylated MOB1 corresponds to better prediction of overall survival in patients with non-small cell lung cancer. Therefore, as demonstrated, an oxidative stress-CBP regulatory axis controls MOB1-K11 acetylation and activates LATS1, thereby activating the Hippo pathway and suppressing YAP/TAZ nuclear translocation and tumor progression. Oxford University Press 2022-03-29 /pmc/articles/PMC9023286/ /pubmed/35349706 http://dx.doi.org/10.1093/nar/gkac189 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Gene regulation, Chromatin and Epigenetics
Jin, Jiaqi
Zhang, Lei
Li, Xueying
Xu, Weizhi
Yang, Siyuan
Song, Jiagui
Zhang, Wenhao
Zhan, Jun
Luo, Jianyuan
Zhang, Hongquan
Oxidative stress-CBP axis modulates MOB1 acetylation and activates the Hippo signaling pathway
title Oxidative stress-CBP axis modulates MOB1 acetylation and activates the Hippo signaling pathway
title_full Oxidative stress-CBP axis modulates MOB1 acetylation and activates the Hippo signaling pathway
title_fullStr Oxidative stress-CBP axis modulates MOB1 acetylation and activates the Hippo signaling pathway
title_full_unstemmed Oxidative stress-CBP axis modulates MOB1 acetylation and activates the Hippo signaling pathway
title_short Oxidative stress-CBP axis modulates MOB1 acetylation and activates the Hippo signaling pathway
title_sort oxidative stress-cbp axis modulates mob1 acetylation and activates the hippo signaling pathway
topic Gene regulation, Chromatin and Epigenetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9023286/
https://www.ncbi.nlm.nih.gov/pubmed/35349706
http://dx.doi.org/10.1093/nar/gkac189
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