Imperfect guide-RNA (igRNA) enables CRISPR single-base editing with ABE and CBE

CRISPR base editing techniques tend to edit multiple bases in the targeted region, which is a limitation for precisely reverting disease-associated single-nucleotide polymorphisms (SNPs). We designed an imperfect gRNA (igRNA) editing methodology, which utilized a gRNA with one or more bases that wer...

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Detalles Bibliográficos
Autores principales: Zhao, Dongdong, Jiang, Guo, Li, Ju, Chen, Xuxu, Li, Siwei, Wang, Jie, Zhou, Zuping, Pu, Shiming, Dai, Zhubo, Ma, Yanhe, Bi, Changhao, Zhang, Xueli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Synthetic Biology and Bioengineering
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9023296/
https://www.ncbi.nlm.nih.gov/pubmed/35349689
http://dx.doi.org/10.1093/nar/gkac201

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