Real-time cell analysis: A high-throughput approach for testing SARS-CoV-2 antibody neutralization and escape

Real-time cell analysis (RTCA) enables high-throughput, quantitative kinetic measurements of cytopathic effect (CPE) in virus-infected cells. Here, we detail a RTCA approach for assessing antibody neutralization. We describe how to evaluate the neutralizing potency of monoclonal antibodies (mAbs) an...

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Detalles Bibliográficos
Autores principales: Suryadevara, Naveenchandra, Gilchuk, Pavlo, Zost, Seth J., Mittal, Nikhil, Zhao, Li Leyna, Crowe, James E., Carnahan, Robert H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9023333/
https://www.ncbi.nlm.nih.gov/pubmed/35578733
http://dx.doi.org/10.1016/j.xpro.2022.101387
Descripción
Sumario:Real-time cell analysis (RTCA) enables high-throughput, quantitative kinetic measurements of cytopathic effect (CPE) in virus-infected cells. Here, we detail a RTCA approach for assessing antibody neutralization. We describe how to evaluate the neutralizing potency of monoclonal antibodies (mAbs) and identify viral escape mutants to antibody neutralization for severe respiratory syndrome coronavirus 2 (SARS-CoV-2). For complete details on the use and execution of this protocol, please refer to Zost et al. (2020) and Suryadevara et al. (2021).

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