Real-time cell analysis: A high-throughput approach for testing SARS-CoV-2 antibody neutralization and escape

Real-time cell analysis (RTCA) enables high-throughput, quantitative kinetic measurements of cytopathic effect (CPE) in virus-infected cells. Here, we detail a RTCA approach for assessing antibody neutralization. We describe how to evaluate the neutralizing potency of monoclonal antibodies (mAbs) an...

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Autores principales: Suryadevara, Naveenchandra, Gilchuk, Pavlo, Zost, Seth J., Mittal, Nikhil, Zhao, Li Leyna, Crowe, James E., Carnahan, Robert H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9023333/
https://www.ncbi.nlm.nih.gov/pubmed/35578733
http://dx.doi.org/10.1016/j.xpro.2022.101387
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author Suryadevara, Naveenchandra
Gilchuk, Pavlo
Zost, Seth J.
Mittal, Nikhil
Zhao, Li Leyna
Crowe, James E.
Carnahan, Robert H.
author_facet Suryadevara, Naveenchandra
Gilchuk, Pavlo
Zost, Seth J.
Mittal, Nikhil
Zhao, Li Leyna
Crowe, James E.
Carnahan, Robert H.
author_sort Suryadevara, Naveenchandra
collection PubMed
description Real-time cell analysis (RTCA) enables high-throughput, quantitative kinetic measurements of cytopathic effect (CPE) in virus-infected cells. Here, we detail a RTCA approach for assessing antibody neutralization. We describe how to evaluate the neutralizing potency of monoclonal antibodies (mAbs) and identify viral escape mutants to antibody neutralization for severe respiratory syndrome coronavirus 2 (SARS-CoV-2). For complete details on the use and execution of this protocol, please refer to Zost et al. (2020) and Suryadevara et al. (2021).
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spelling pubmed-90233332022-04-22 Real-time cell analysis: A high-throughput approach for testing SARS-CoV-2 antibody neutralization and escape Suryadevara, Naveenchandra Gilchuk, Pavlo Zost, Seth J. Mittal, Nikhil Zhao, Li Leyna Crowe, James E. Carnahan, Robert H. STAR Protoc Protocol Real-time cell analysis (RTCA) enables high-throughput, quantitative kinetic measurements of cytopathic effect (CPE) in virus-infected cells. Here, we detail a RTCA approach for assessing antibody neutralization. We describe how to evaluate the neutralizing potency of monoclonal antibodies (mAbs) and identify viral escape mutants to antibody neutralization for severe respiratory syndrome coronavirus 2 (SARS-CoV-2). For complete details on the use and execution of this protocol, please refer to Zost et al. (2020) and Suryadevara et al. (2021). Elsevier 2022-04-22 /pmc/articles/PMC9023333/ /pubmed/35578733 http://dx.doi.org/10.1016/j.xpro.2022.101387 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Suryadevara, Naveenchandra
Gilchuk, Pavlo
Zost, Seth J.
Mittal, Nikhil
Zhao, Li Leyna
Crowe, James E.
Carnahan, Robert H.
Real-time cell analysis: A high-throughput approach for testing SARS-CoV-2 antibody neutralization and escape
title Real-time cell analysis: A high-throughput approach for testing SARS-CoV-2 antibody neutralization and escape
title_full Real-time cell analysis: A high-throughput approach for testing SARS-CoV-2 antibody neutralization and escape
title_fullStr Real-time cell analysis: A high-throughput approach for testing SARS-CoV-2 antibody neutralization and escape
title_full_unstemmed Real-time cell analysis: A high-throughput approach for testing SARS-CoV-2 antibody neutralization and escape
title_short Real-time cell analysis: A high-throughput approach for testing SARS-CoV-2 antibody neutralization and escape
title_sort real-time cell analysis: a high-throughput approach for testing sars-cov-2 antibody neutralization and escape
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9023333/
https://www.ncbi.nlm.nih.gov/pubmed/35578733
http://dx.doi.org/10.1016/j.xpro.2022.101387
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