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Next generation automated traceless cell chromatography platform for GMP-compliant cell isolation and activation
Large-scale target cell isolation from patient blood preparations is one of the critical operations during drug product manufacturing for personalized cell therapy in immuno-oncology. Use of high-affinity murine antibody coated magnetic nanoparticles that remain on isolated cells is the current stan...
Autores principales: | , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9023455/ https://www.ncbi.nlm.nih.gov/pubmed/35449227 http://dx.doi.org/10.1038/s41598-022-10320-x |
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author | Radisch, Sabine Poltorak, Mateusz P. Wagner, Michaela Cletiu, Vlad Radisch, Christian Treise, Irina Pann, Steffi Weigt, Alexis Artner, Sophie Dreher, Stefan Fechner, Fabian Borjan, Bojana Fraessle, Simon P. Effenberger, Manuel Benke, Eileen Navratil, Gottfried Hentschel, Norbert Busch, Dirk H. Schmidt, Thomas Stemberger, Christian Germeroth, Lothar |
author_facet | Radisch, Sabine Poltorak, Mateusz P. Wagner, Michaela Cletiu, Vlad Radisch, Christian Treise, Irina Pann, Steffi Weigt, Alexis Artner, Sophie Dreher, Stefan Fechner, Fabian Borjan, Bojana Fraessle, Simon P. Effenberger, Manuel Benke, Eileen Navratil, Gottfried Hentschel, Norbert Busch, Dirk H. Schmidt, Thomas Stemberger, Christian Germeroth, Lothar |
author_sort | Radisch, Sabine |
collection | PubMed |
description | Large-scale target cell isolation from patient blood preparations is one of the critical operations during drug product manufacturing for personalized cell therapy in immuno-oncology. Use of high-affinity murine antibody coated magnetic nanoparticles that remain on isolated cells is the current standard applied for this purpose. Here, we present the transformation of previously described technology — non-magnetic immunoaffinity column chromatography-based cell selection with reversible reagents into a new clinical-grade cell isolation platform called Automated Traceless Cell affinity chromatography (ATC). ATC is a fully closed and GMP-compliant cell selection and manufacturing system. Reversibility of reagents enables (sequential) positive cell selection, optionally in combination with depletion columns, enabling capture of highly specific cell subsets. Moreover, synergy with other Streptamer-based technologies allows novel uses beyond cell isolation including integrated and automated on-column target cell activation. In conclusion, ATC technology is an innovative as well as versatile platform to select, stimulate and modify cells for clinical manufacturing and downstream therapies. |
format | Online Article Text |
id | pubmed-9023455 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-90234552022-04-25 Next generation automated traceless cell chromatography platform for GMP-compliant cell isolation and activation Radisch, Sabine Poltorak, Mateusz P. Wagner, Michaela Cletiu, Vlad Radisch, Christian Treise, Irina Pann, Steffi Weigt, Alexis Artner, Sophie Dreher, Stefan Fechner, Fabian Borjan, Bojana Fraessle, Simon P. Effenberger, Manuel Benke, Eileen Navratil, Gottfried Hentschel, Norbert Busch, Dirk H. Schmidt, Thomas Stemberger, Christian Germeroth, Lothar Sci Rep Article Large-scale target cell isolation from patient blood preparations is one of the critical operations during drug product manufacturing for personalized cell therapy in immuno-oncology. Use of high-affinity murine antibody coated magnetic nanoparticles that remain on isolated cells is the current standard applied for this purpose. Here, we present the transformation of previously described technology — non-magnetic immunoaffinity column chromatography-based cell selection with reversible reagents into a new clinical-grade cell isolation platform called Automated Traceless Cell affinity chromatography (ATC). ATC is a fully closed and GMP-compliant cell selection and manufacturing system. Reversibility of reagents enables (sequential) positive cell selection, optionally in combination with depletion columns, enabling capture of highly specific cell subsets. Moreover, synergy with other Streptamer-based technologies allows novel uses beyond cell isolation including integrated and automated on-column target cell activation. In conclusion, ATC technology is an innovative as well as versatile platform to select, stimulate and modify cells for clinical manufacturing and downstream therapies. Nature Publishing Group UK 2022-04-21 /pmc/articles/PMC9023455/ /pubmed/35449227 http://dx.doi.org/10.1038/s41598-022-10320-x Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Radisch, Sabine Poltorak, Mateusz P. Wagner, Michaela Cletiu, Vlad Radisch, Christian Treise, Irina Pann, Steffi Weigt, Alexis Artner, Sophie Dreher, Stefan Fechner, Fabian Borjan, Bojana Fraessle, Simon P. Effenberger, Manuel Benke, Eileen Navratil, Gottfried Hentschel, Norbert Busch, Dirk H. Schmidt, Thomas Stemberger, Christian Germeroth, Lothar Next generation automated traceless cell chromatography platform for GMP-compliant cell isolation and activation |
title | Next generation automated traceless cell chromatography platform for GMP-compliant cell isolation and activation |
title_full | Next generation automated traceless cell chromatography platform for GMP-compliant cell isolation and activation |
title_fullStr | Next generation automated traceless cell chromatography platform for GMP-compliant cell isolation and activation |
title_full_unstemmed | Next generation automated traceless cell chromatography platform for GMP-compliant cell isolation and activation |
title_short | Next generation automated traceless cell chromatography platform for GMP-compliant cell isolation and activation |
title_sort | next generation automated traceless cell chromatography platform for gmp-compliant cell isolation and activation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9023455/ https://www.ncbi.nlm.nih.gov/pubmed/35449227 http://dx.doi.org/10.1038/s41598-022-10320-x |
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