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Next generation automated traceless cell chromatography platform for GMP-compliant cell isolation and activation

Large-scale target cell isolation from patient blood preparations is one of the critical operations during drug product manufacturing for personalized cell therapy in immuno-oncology. Use of high-affinity murine antibody coated magnetic nanoparticles that remain on isolated cells is the current stan...

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Autores principales: Radisch, Sabine, Poltorak, Mateusz P., Wagner, Michaela, Cletiu, Vlad, Radisch, Christian, Treise, Irina, Pann, Steffi, Weigt, Alexis, Artner, Sophie, Dreher, Stefan, Fechner, Fabian, Borjan, Bojana, Fraessle, Simon P., Effenberger, Manuel, Benke, Eileen, Navratil, Gottfried, Hentschel, Norbert, Busch, Dirk H., Schmidt, Thomas, Stemberger, Christian, Germeroth, Lothar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9023455/
https://www.ncbi.nlm.nih.gov/pubmed/35449227
http://dx.doi.org/10.1038/s41598-022-10320-x
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author Radisch, Sabine
Poltorak, Mateusz P.
Wagner, Michaela
Cletiu, Vlad
Radisch, Christian
Treise, Irina
Pann, Steffi
Weigt, Alexis
Artner, Sophie
Dreher, Stefan
Fechner, Fabian
Borjan, Bojana
Fraessle, Simon P.
Effenberger, Manuel
Benke, Eileen
Navratil, Gottfried
Hentschel, Norbert
Busch, Dirk H.
Schmidt, Thomas
Stemberger, Christian
Germeroth, Lothar
author_facet Radisch, Sabine
Poltorak, Mateusz P.
Wagner, Michaela
Cletiu, Vlad
Radisch, Christian
Treise, Irina
Pann, Steffi
Weigt, Alexis
Artner, Sophie
Dreher, Stefan
Fechner, Fabian
Borjan, Bojana
Fraessle, Simon P.
Effenberger, Manuel
Benke, Eileen
Navratil, Gottfried
Hentschel, Norbert
Busch, Dirk H.
Schmidt, Thomas
Stemberger, Christian
Germeroth, Lothar
author_sort Radisch, Sabine
collection PubMed
description Large-scale target cell isolation from patient blood preparations is one of the critical operations during drug product manufacturing for personalized cell therapy in immuno-oncology. Use of high-affinity murine antibody coated magnetic nanoparticles that remain on isolated cells is the current standard applied for this purpose. Here, we present the transformation of previously described technology — non-magnetic immunoaffinity column chromatography-based cell selection with reversible reagents into a new clinical-grade cell isolation platform called Automated Traceless Cell affinity chromatography (ATC). ATC is a fully closed and GMP-compliant cell selection and manufacturing system. Reversibility of reagents enables (sequential) positive cell selection, optionally in combination with depletion columns, enabling capture of highly specific cell subsets. Moreover, synergy with other Streptamer-based technologies allows novel uses beyond cell isolation including integrated and automated on-column target cell activation. In conclusion, ATC technology is an innovative as well as versatile platform to select, stimulate and modify cells for clinical manufacturing and downstream therapies.
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spelling pubmed-90234552022-04-25 Next generation automated traceless cell chromatography platform for GMP-compliant cell isolation and activation Radisch, Sabine Poltorak, Mateusz P. Wagner, Michaela Cletiu, Vlad Radisch, Christian Treise, Irina Pann, Steffi Weigt, Alexis Artner, Sophie Dreher, Stefan Fechner, Fabian Borjan, Bojana Fraessle, Simon P. Effenberger, Manuel Benke, Eileen Navratil, Gottfried Hentschel, Norbert Busch, Dirk H. Schmidt, Thomas Stemberger, Christian Germeroth, Lothar Sci Rep Article Large-scale target cell isolation from patient blood preparations is one of the critical operations during drug product manufacturing for personalized cell therapy in immuno-oncology. Use of high-affinity murine antibody coated magnetic nanoparticles that remain on isolated cells is the current standard applied for this purpose. Here, we present the transformation of previously described technology — non-magnetic immunoaffinity column chromatography-based cell selection with reversible reagents into a new clinical-grade cell isolation platform called Automated Traceless Cell affinity chromatography (ATC). ATC is a fully closed and GMP-compliant cell selection and manufacturing system. Reversibility of reagents enables (sequential) positive cell selection, optionally in combination with depletion columns, enabling capture of highly specific cell subsets. Moreover, synergy with other Streptamer-based technologies allows novel uses beyond cell isolation including integrated and automated on-column target cell activation. In conclusion, ATC technology is an innovative as well as versatile platform to select, stimulate and modify cells for clinical manufacturing and downstream therapies. Nature Publishing Group UK 2022-04-21 /pmc/articles/PMC9023455/ /pubmed/35449227 http://dx.doi.org/10.1038/s41598-022-10320-x Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Radisch, Sabine
Poltorak, Mateusz P.
Wagner, Michaela
Cletiu, Vlad
Radisch, Christian
Treise, Irina
Pann, Steffi
Weigt, Alexis
Artner, Sophie
Dreher, Stefan
Fechner, Fabian
Borjan, Bojana
Fraessle, Simon P.
Effenberger, Manuel
Benke, Eileen
Navratil, Gottfried
Hentschel, Norbert
Busch, Dirk H.
Schmidt, Thomas
Stemberger, Christian
Germeroth, Lothar
Next generation automated traceless cell chromatography platform for GMP-compliant cell isolation and activation
title Next generation automated traceless cell chromatography platform for GMP-compliant cell isolation and activation
title_full Next generation automated traceless cell chromatography platform for GMP-compliant cell isolation and activation
title_fullStr Next generation automated traceless cell chromatography platform for GMP-compliant cell isolation and activation
title_full_unstemmed Next generation automated traceless cell chromatography platform for GMP-compliant cell isolation and activation
title_short Next generation automated traceless cell chromatography platform for GMP-compliant cell isolation and activation
title_sort next generation automated traceless cell chromatography platform for gmp-compliant cell isolation and activation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9023455/
https://www.ncbi.nlm.nih.gov/pubmed/35449227
http://dx.doi.org/10.1038/s41598-022-10320-x
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