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Establishment of a fish model to study gas-bubble lesions
Decompression sickness (DCS) is a clinical syndrome caused by the formation of systemic intravascular and extravascular gas bubbles. The presence of these bubbles in blood vessels is known as gas embolism. DCS has been described in humans and animals such as sea turtles and cetaceans. To delve deepe...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9023494/ https://www.ncbi.nlm.nih.gov/pubmed/35449183 http://dx.doi.org/10.1038/s41598-022-10539-8 |
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author | Velázquez-Wallraf, Alicia Fernández, Antonio Caballero, María José Arregui, Marina González Díaz, Óscar Betancor, Mónica B. Bernaldo de Quirós, Yara |
author_facet | Velázquez-Wallraf, Alicia Fernández, Antonio Caballero, María José Arregui, Marina González Díaz, Óscar Betancor, Mónica B. Bernaldo de Quirós, Yara |
author_sort | Velázquez-Wallraf, Alicia |
collection | PubMed |
description | Decompression sickness (DCS) is a clinical syndrome caused by the formation of systemic intravascular and extravascular gas bubbles. The presence of these bubbles in blood vessels is known as gas embolism. DCS has been described in humans and animals such as sea turtles and cetaceans. To delve deeper into DCS, experimental models in terrestrial mammals subjected to compression/decompression in a hyperbaric chamber have been used. Fish can suffer from gas bubble disease (GBD), characterized by the formation of intravascular and extravascular systemic gas bubbles, similarly to that observed in DCS. Given these similarities and the fact that fish develop this disease naturally in supersaturated water, they could be used as an alternative experimental model for the study of the pathophysiological aspect of gas bubbles. The objective of this study was to obtain a reproducible model for GBD in fish by an engineering system and a complete pathological study, validating this model for the study of the physiopathology of gas related lesions in DCS. A massive and severe GBD was achieved by exposing the fish for 18 h to TDG values of 162–163%, characterized by the presence of severe hemorrhages and the visualization of massive quantities of macroscopic and microscopic gas bubbles, systemically distributed, circulating through different large vessels of experimental fish. These pathological findings were the same as those described in small mammals for the study of explosive DCS by hyperbaric chamber, validating the translational usefulness of this first fish model to study the gas-bubbles lesions associated to DCS from a pathological standpoint. |
format | Online Article Text |
id | pubmed-9023494 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-90234942022-04-25 Establishment of a fish model to study gas-bubble lesions Velázquez-Wallraf, Alicia Fernández, Antonio Caballero, María José Arregui, Marina González Díaz, Óscar Betancor, Mónica B. Bernaldo de Quirós, Yara Sci Rep Article Decompression sickness (DCS) is a clinical syndrome caused by the formation of systemic intravascular and extravascular gas bubbles. The presence of these bubbles in blood vessels is known as gas embolism. DCS has been described in humans and animals such as sea turtles and cetaceans. To delve deeper into DCS, experimental models in terrestrial mammals subjected to compression/decompression in a hyperbaric chamber have been used. Fish can suffer from gas bubble disease (GBD), characterized by the formation of intravascular and extravascular systemic gas bubbles, similarly to that observed in DCS. Given these similarities and the fact that fish develop this disease naturally in supersaturated water, they could be used as an alternative experimental model for the study of the pathophysiological aspect of gas bubbles. The objective of this study was to obtain a reproducible model for GBD in fish by an engineering system and a complete pathological study, validating this model for the study of the physiopathology of gas related lesions in DCS. A massive and severe GBD was achieved by exposing the fish for 18 h to TDG values of 162–163%, characterized by the presence of severe hemorrhages and the visualization of massive quantities of macroscopic and microscopic gas bubbles, systemically distributed, circulating through different large vessels of experimental fish. These pathological findings were the same as those described in small mammals for the study of explosive DCS by hyperbaric chamber, validating the translational usefulness of this first fish model to study the gas-bubbles lesions associated to DCS from a pathological standpoint. Nature Publishing Group UK 2022-04-21 /pmc/articles/PMC9023494/ /pubmed/35449183 http://dx.doi.org/10.1038/s41598-022-10539-8 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Velázquez-Wallraf, Alicia Fernández, Antonio Caballero, María José Arregui, Marina González Díaz, Óscar Betancor, Mónica B. Bernaldo de Quirós, Yara Establishment of a fish model to study gas-bubble lesions |
title | Establishment of a fish model to study gas-bubble lesions |
title_full | Establishment of a fish model to study gas-bubble lesions |
title_fullStr | Establishment of a fish model to study gas-bubble lesions |
title_full_unstemmed | Establishment of a fish model to study gas-bubble lesions |
title_short | Establishment of a fish model to study gas-bubble lesions |
title_sort | establishment of a fish model to study gas-bubble lesions |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9023494/ https://www.ncbi.nlm.nih.gov/pubmed/35449183 http://dx.doi.org/10.1038/s41598-022-10539-8 |
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