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Redesigning regulatory components of quorum-sensing system for diverse metabolic control
Quorum sensing (QS) is a ubiquitous cell–cell communication mechanism that can be employed to autonomously and dynamically control metabolic fluxes. However, since the functions of genetic components in the circuits are not fully understood, the developed QS circuits are still less sophisticated for...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9023504/ https://www.ncbi.nlm.nih.gov/pubmed/35449138 http://dx.doi.org/10.1038/s41467-022-29933-x |
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author | Ge, Chang Yu, Zheng Sheng, Huakang Shen, Xiaolin Sun, Xinxiao Zhang, Yifei Yan, Yajun Wang, Jia Yuan, Qipeng |
author_facet | Ge, Chang Yu, Zheng Sheng, Huakang Shen, Xiaolin Sun, Xinxiao Zhang, Yifei Yan, Yajun Wang, Jia Yuan, Qipeng |
author_sort | Ge, Chang |
collection | PubMed |
description | Quorum sensing (QS) is a ubiquitous cell–cell communication mechanism that can be employed to autonomously and dynamically control metabolic fluxes. However, since the functions of genetic components in the circuits are not fully understood, the developed QS circuits are still less sophisticated for regulating multiple sets of genes or operons in metabolic engineering applications. Here, we discover the regulatory roles of a CRP-binding site and the lux box to −10 region within luxR-luxI intergenic sequence in controlling the lux-type QS promoters. By varying the numbers of the CRP-binding site and redesigning the lux box to −10 site sequence, we create a library of QS variants that possess both high dynamic ranges and low leakiness. These circuits are successfully applied to achieve diverse metabolic control in salicylic acid and 4-hydroxycoumarin biosynthetic pathways in Escherichia coli. This work expands the toolbox for dynamic control of multiple metabolic fluxes under complex metabolic background and presents paradigms to engineer metabolic pathways for high-level synthesis of target products. |
format | Online Article Text |
id | pubmed-9023504 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-90235042022-04-28 Redesigning regulatory components of quorum-sensing system for diverse metabolic control Ge, Chang Yu, Zheng Sheng, Huakang Shen, Xiaolin Sun, Xinxiao Zhang, Yifei Yan, Yajun Wang, Jia Yuan, Qipeng Nat Commun Article Quorum sensing (QS) is a ubiquitous cell–cell communication mechanism that can be employed to autonomously and dynamically control metabolic fluxes. However, since the functions of genetic components in the circuits are not fully understood, the developed QS circuits are still less sophisticated for regulating multiple sets of genes or operons in metabolic engineering applications. Here, we discover the regulatory roles of a CRP-binding site and the lux box to −10 region within luxR-luxI intergenic sequence in controlling the lux-type QS promoters. By varying the numbers of the CRP-binding site and redesigning the lux box to −10 site sequence, we create a library of QS variants that possess both high dynamic ranges and low leakiness. These circuits are successfully applied to achieve diverse metabolic control in salicylic acid and 4-hydroxycoumarin biosynthetic pathways in Escherichia coli. This work expands the toolbox for dynamic control of multiple metabolic fluxes under complex metabolic background and presents paradigms to engineer metabolic pathways for high-level synthesis of target products. Nature Publishing Group UK 2022-04-21 /pmc/articles/PMC9023504/ /pubmed/35449138 http://dx.doi.org/10.1038/s41467-022-29933-x Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Ge, Chang Yu, Zheng Sheng, Huakang Shen, Xiaolin Sun, Xinxiao Zhang, Yifei Yan, Yajun Wang, Jia Yuan, Qipeng Redesigning regulatory components of quorum-sensing system for diverse metabolic control |
title | Redesigning regulatory components of quorum-sensing system for diverse metabolic control |
title_full | Redesigning regulatory components of quorum-sensing system for diverse metabolic control |
title_fullStr | Redesigning regulatory components of quorum-sensing system for diverse metabolic control |
title_full_unstemmed | Redesigning regulatory components of quorum-sensing system for diverse metabolic control |
title_short | Redesigning regulatory components of quorum-sensing system for diverse metabolic control |
title_sort | redesigning regulatory components of quorum-sensing system for diverse metabolic control |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9023504/ https://www.ncbi.nlm.nih.gov/pubmed/35449138 http://dx.doi.org/10.1038/s41467-022-29933-x |
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