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Comprehending Cardiac Dysfunction by Oxidative Stress: Untargeted Metabolomics of In Vitro Samples
Cardiovascular diseases (CVDs) are noncommunicable diseases known for their complex etiology and high mortality rate. Oxidative stress (OS), a condition in which the release of free radical exceeds endogenous antioxidant capacity, is pivotal in CVC, such as myocardial infarction, ischemia/reperfusio...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9023746/ https://www.ncbi.nlm.nih.gov/pubmed/35464220 http://dx.doi.org/10.3389/fchem.2022.836478 |
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author | Amaral, Alan Gonçalves Moretto, Isabela Aparecida Zandonadi, Flávia da Silva Zamora-Obando, Hans Rolando Rocha, Isabela Sussulini, Alessandra de Thomaz, André Alexandre Oliveira, Regina Vincenzi dos Santos, Aline Mara Simionato, Ana Valéria Colnaghi |
author_facet | Amaral, Alan Gonçalves Moretto, Isabela Aparecida Zandonadi, Flávia da Silva Zamora-Obando, Hans Rolando Rocha, Isabela Sussulini, Alessandra de Thomaz, André Alexandre Oliveira, Regina Vincenzi dos Santos, Aline Mara Simionato, Ana Valéria Colnaghi |
author_sort | Amaral, Alan Gonçalves |
collection | PubMed |
description | Cardiovascular diseases (CVDs) are noncommunicable diseases known for their complex etiology and high mortality rate. Oxidative stress (OS), a condition in which the release of free radical exceeds endogenous antioxidant capacity, is pivotal in CVC, such as myocardial infarction, ischemia/reperfusion, and heart failure. Due to the lack of information about the implications of OS on cardiovascular conditions, several methodologies have been applied to investigate the causes and consequences, and to find new ways of diagnosis and treatment as well. In the present study, cardiac dysfunction was evaluated by analyzing cells’ alterations with untargeted metabolomics, after simulation of an oxidative stress condition using hydrogen peroxide (H(2)O(2)) in H9c2 myocytes. Optimizations of H(2)O(2) concentration, cell exposure, and cell recovery times were performed through MTT assays. Intracellular metabolites were analyzed right after the oxidative stress (oxidative stress group) and after 48 h of cell recovery (recovery group) by ultra-high-performance liquid chromatography coupled to mass spectrometry (UHPLC-MS) in positive and negative ESI ionization mode. Significant alterations were found in pathways such as “alanine, aspartate and glutamate metabolism”, “glycolysis”, and “glutathione metabolism”, mostly with increased metabolites (upregulated). Furthermore, our results indicated that the LC-MS method is effective for studying metabolism in cardiomyocytes and generated excellent fit (R(2)Y > 0.987) and predictability (Q(2) > 0.84) values. |
format | Online Article Text |
id | pubmed-9023746 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-90237462022-04-23 Comprehending Cardiac Dysfunction by Oxidative Stress: Untargeted Metabolomics of In Vitro Samples Amaral, Alan Gonçalves Moretto, Isabela Aparecida Zandonadi, Flávia da Silva Zamora-Obando, Hans Rolando Rocha, Isabela Sussulini, Alessandra de Thomaz, André Alexandre Oliveira, Regina Vincenzi dos Santos, Aline Mara Simionato, Ana Valéria Colnaghi Front Chem Chemistry Cardiovascular diseases (CVDs) are noncommunicable diseases known for their complex etiology and high mortality rate. Oxidative stress (OS), a condition in which the release of free radical exceeds endogenous antioxidant capacity, is pivotal in CVC, such as myocardial infarction, ischemia/reperfusion, and heart failure. Due to the lack of information about the implications of OS on cardiovascular conditions, several methodologies have been applied to investigate the causes and consequences, and to find new ways of diagnosis and treatment as well. In the present study, cardiac dysfunction was evaluated by analyzing cells’ alterations with untargeted metabolomics, after simulation of an oxidative stress condition using hydrogen peroxide (H(2)O(2)) in H9c2 myocytes. Optimizations of H(2)O(2) concentration, cell exposure, and cell recovery times were performed through MTT assays. Intracellular metabolites were analyzed right after the oxidative stress (oxidative stress group) and after 48 h of cell recovery (recovery group) by ultra-high-performance liquid chromatography coupled to mass spectrometry (UHPLC-MS) in positive and negative ESI ionization mode. Significant alterations were found in pathways such as “alanine, aspartate and glutamate metabolism”, “glycolysis”, and “glutathione metabolism”, mostly with increased metabolites (upregulated). Furthermore, our results indicated that the LC-MS method is effective for studying metabolism in cardiomyocytes and generated excellent fit (R(2)Y > 0.987) and predictability (Q(2) > 0.84) values. Frontiers Media S.A. 2022-04-08 /pmc/articles/PMC9023746/ /pubmed/35464220 http://dx.doi.org/10.3389/fchem.2022.836478 Text en Copyright © 2022 Amaral, Moretto, Zandonadi, Zamora-Obando, Rocha, Sussulini, Thomaz, Oliveira, Santos and Simionato. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Chemistry Amaral, Alan Gonçalves Moretto, Isabela Aparecida Zandonadi, Flávia da Silva Zamora-Obando, Hans Rolando Rocha, Isabela Sussulini, Alessandra de Thomaz, André Alexandre Oliveira, Regina Vincenzi dos Santos, Aline Mara Simionato, Ana Valéria Colnaghi Comprehending Cardiac Dysfunction by Oxidative Stress: Untargeted Metabolomics of In Vitro Samples |
title | Comprehending Cardiac Dysfunction by Oxidative Stress: Untargeted Metabolomics of In Vitro Samples |
title_full | Comprehending Cardiac Dysfunction by Oxidative Stress: Untargeted Metabolomics of In Vitro Samples |
title_fullStr | Comprehending Cardiac Dysfunction by Oxidative Stress: Untargeted Metabolomics of In Vitro Samples |
title_full_unstemmed | Comprehending Cardiac Dysfunction by Oxidative Stress: Untargeted Metabolomics of In Vitro Samples |
title_short | Comprehending Cardiac Dysfunction by Oxidative Stress: Untargeted Metabolomics of In Vitro Samples |
title_sort | comprehending cardiac dysfunction by oxidative stress: untargeted metabolomics of in vitro samples |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9023746/ https://www.ncbi.nlm.nih.gov/pubmed/35464220 http://dx.doi.org/10.3389/fchem.2022.836478 |
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