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Transcriptional profiling of β-2M(−)SPα-6(+)THY1(+) spermatogonial stem cells in human spermatogenesis
Male infertility is responsible for approximately half of all cases of reproductive issues. Spermatogenesis originates in a small pool of spermatogonial stem cells (SSCs), which are of interest for therapy of infertility but remain not well defined in humans. Using multiparametric analysis of the si...
Autores principales: | , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9023810/ https://www.ncbi.nlm.nih.gov/pubmed/35334216 http://dx.doi.org/10.1016/j.stemcr.2022.02.017 |
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author | Givelet, Maelle Firlej, Virginie Lassalle, Bruno Gille, Anne Sophie Lapoujade, Clementine Holtzman, Isabelle Jarysta, Amandine Haghighirad, Farahd Dumont, Florent Jacques, Sébastien Letourneur, Franck Pflumio, Françoise Allemand, Isabelle Patrat, Catherine Thiounn, Nicolas Wolf, Jean Philippe Riou, Lydia Barraud-Lange, Virginie Fouchet, Pierre |
author_facet | Givelet, Maelle Firlej, Virginie Lassalle, Bruno Gille, Anne Sophie Lapoujade, Clementine Holtzman, Isabelle Jarysta, Amandine Haghighirad, Farahd Dumont, Florent Jacques, Sébastien Letourneur, Franck Pflumio, Françoise Allemand, Isabelle Patrat, Catherine Thiounn, Nicolas Wolf, Jean Philippe Riou, Lydia Barraud-Lange, Virginie Fouchet, Pierre |
author_sort | Givelet, Maelle |
collection | PubMed |
description | Male infertility is responsible for approximately half of all cases of reproductive issues. Spermatogenesis originates in a small pool of spermatogonial stem cells (SSCs), which are of interest for therapy of infertility but remain not well defined in humans. Using multiparametric analysis of the side population (SP) phenotype and the α-6 integrin, THY1, and β-2 microglobulin cell markers, we identified a population of human primitive undifferentiated spermatogonia with the phenotype β-2 microglobulin (β-2M)(−)SPα-6(+)THY1(+), which is highly enriched in stem cells. By analyzing the expression signatures of this SSC-enriched population along with other germinal progenitors, we established an exhaustive transcriptome of human spermatogenesis. Transcriptome profiling of the human β-2M(−)SPα-6(+)THY1(+) population and comparison with the profile of mouse undifferentiated spermatogonia provide insights into the molecular networks and key transcriptional regulators regulating human SSCs, including the basic-helix-loop-helix (bHLH) transcriptional repressor HES1, which we show to be implicated in maintenance of SSCs in vitro. |
format | Online Article Text |
id | pubmed-9023810 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-90238102022-04-23 Transcriptional profiling of β-2M(−)SPα-6(+)THY1(+) spermatogonial stem cells in human spermatogenesis Givelet, Maelle Firlej, Virginie Lassalle, Bruno Gille, Anne Sophie Lapoujade, Clementine Holtzman, Isabelle Jarysta, Amandine Haghighirad, Farahd Dumont, Florent Jacques, Sébastien Letourneur, Franck Pflumio, Françoise Allemand, Isabelle Patrat, Catherine Thiounn, Nicolas Wolf, Jean Philippe Riou, Lydia Barraud-Lange, Virginie Fouchet, Pierre Stem Cell Reports Resource Male infertility is responsible for approximately half of all cases of reproductive issues. Spermatogenesis originates in a small pool of spermatogonial stem cells (SSCs), which are of interest for therapy of infertility but remain not well defined in humans. Using multiparametric analysis of the side population (SP) phenotype and the α-6 integrin, THY1, and β-2 microglobulin cell markers, we identified a population of human primitive undifferentiated spermatogonia with the phenotype β-2 microglobulin (β-2M)(−)SPα-6(+)THY1(+), which is highly enriched in stem cells. By analyzing the expression signatures of this SSC-enriched population along with other germinal progenitors, we established an exhaustive transcriptome of human spermatogenesis. Transcriptome profiling of the human β-2M(−)SPα-6(+)THY1(+) population and comparison with the profile of mouse undifferentiated spermatogonia provide insights into the molecular networks and key transcriptional regulators regulating human SSCs, including the basic-helix-loop-helix (bHLH) transcriptional repressor HES1, which we show to be implicated in maintenance of SSCs in vitro. Elsevier 2022-03-24 /pmc/articles/PMC9023810/ /pubmed/35334216 http://dx.doi.org/10.1016/j.stemcr.2022.02.017 Text en © 2022 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Resource Givelet, Maelle Firlej, Virginie Lassalle, Bruno Gille, Anne Sophie Lapoujade, Clementine Holtzman, Isabelle Jarysta, Amandine Haghighirad, Farahd Dumont, Florent Jacques, Sébastien Letourneur, Franck Pflumio, Françoise Allemand, Isabelle Patrat, Catherine Thiounn, Nicolas Wolf, Jean Philippe Riou, Lydia Barraud-Lange, Virginie Fouchet, Pierre Transcriptional profiling of β-2M(−)SPα-6(+)THY1(+) spermatogonial stem cells in human spermatogenesis |
title | Transcriptional profiling of β-2M(−)SPα-6(+)THY1(+) spermatogonial stem cells in human spermatogenesis |
title_full | Transcriptional profiling of β-2M(−)SPα-6(+)THY1(+) spermatogonial stem cells in human spermatogenesis |
title_fullStr | Transcriptional profiling of β-2M(−)SPα-6(+)THY1(+) spermatogonial stem cells in human spermatogenesis |
title_full_unstemmed | Transcriptional profiling of β-2M(−)SPα-6(+)THY1(+) spermatogonial stem cells in human spermatogenesis |
title_short | Transcriptional profiling of β-2M(−)SPα-6(+)THY1(+) spermatogonial stem cells in human spermatogenesis |
title_sort | transcriptional profiling of β-2m(−)spα-6(+)thy1(+) spermatogonial stem cells in human spermatogenesis |
topic | Resource |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9023810/ https://www.ncbi.nlm.nih.gov/pubmed/35334216 http://dx.doi.org/10.1016/j.stemcr.2022.02.017 |
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