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First Identification and Genetic Characterization of a Novel Duck Astrovirus in Ducklings in China

Four divergent groups of duck astroviruses (DAstVs) have been identified that infect domestic ducks. In March 2021, a fatal disease characterized by visceral urate deposition broke out in 5-day-old Beijing ducks on a commercial farm in Guangdong province, China. We identified a novel duck astrovirus...

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Autores principales: Zhang, Junqin, Huang, Yunzhen, Li, Linlin, Dong, Jiawen, Kuang, Ruihuan, Liao, Ming, Sun, Minhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9024104/
https://www.ncbi.nlm.nih.gov/pubmed/35464380
http://dx.doi.org/10.3389/fvets.2022.873062
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author Zhang, Junqin
Huang, Yunzhen
Li, Linlin
Dong, Jiawen
Kuang, Ruihuan
Liao, Ming
Sun, Minhua
author_facet Zhang, Junqin
Huang, Yunzhen
Li, Linlin
Dong, Jiawen
Kuang, Ruihuan
Liao, Ming
Sun, Minhua
author_sort Zhang, Junqin
collection PubMed
description Four divergent groups of duck astroviruses (DAstVs) have been identified that infect domestic ducks. In March 2021, a fatal disease characterized by visceral urate deposition broke out in 5-day-old Beijing ducks on a commercial farm in Guangdong province, China. We identified a novel duck astrovirus from the ducklings suffering from gout disease. The complete genome sequence of this DAstV was obtained by virome sequencing and amplification. Phylogenetic analyses and pairwise comparisons demonstrated that this DAstV represented a novel group of avastrovirus. Thus, we designated this duck astrovirus as DAstV-5 JM strain. DAstV-5 JM shared genome sequence identities of 15–45% with other avastroviruses. Amino acid identities with proteins from other avastroviruses did not exceed 59% for ORF1a, 79% for ORF1b, and 60% for ORF2. The capsid region of JM shared genetic distances of 0.596 to 0.695 with the three official avastrovirus species. suggesting that JM could be classified as a novel genotype species in the Avastrovirus genus. Meanwhile, JM shares genetic distances of 0.402–0.662 with all the other known unassigned avastroviruses, revealing that it represents an additional unassigned avastrovirus. In summary, we determined that the DAstV-5 JM strain is a novel genotype species of avastrovirus.
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spelling pubmed-90241042022-04-23 First Identification and Genetic Characterization of a Novel Duck Astrovirus in Ducklings in China Zhang, Junqin Huang, Yunzhen Li, Linlin Dong, Jiawen Kuang, Ruihuan Liao, Ming Sun, Minhua Front Vet Sci Veterinary Science Four divergent groups of duck astroviruses (DAstVs) have been identified that infect domestic ducks. In March 2021, a fatal disease characterized by visceral urate deposition broke out in 5-day-old Beijing ducks on a commercial farm in Guangdong province, China. We identified a novel duck astrovirus from the ducklings suffering from gout disease. The complete genome sequence of this DAstV was obtained by virome sequencing and amplification. Phylogenetic analyses and pairwise comparisons demonstrated that this DAstV represented a novel group of avastrovirus. Thus, we designated this duck astrovirus as DAstV-5 JM strain. DAstV-5 JM shared genome sequence identities of 15–45% with other avastroviruses. Amino acid identities with proteins from other avastroviruses did not exceed 59% for ORF1a, 79% for ORF1b, and 60% for ORF2. The capsid region of JM shared genetic distances of 0.596 to 0.695 with the three official avastrovirus species. suggesting that JM could be classified as a novel genotype species in the Avastrovirus genus. Meanwhile, JM shares genetic distances of 0.402–0.662 with all the other known unassigned avastroviruses, revealing that it represents an additional unassigned avastrovirus. In summary, we determined that the DAstV-5 JM strain is a novel genotype species of avastrovirus. Frontiers Media S.A. 2022-04-08 /pmc/articles/PMC9024104/ /pubmed/35464380 http://dx.doi.org/10.3389/fvets.2022.873062 Text en Copyright © 2022 Zhang, Huang, Li, Dong, Kuang, Liao and Sun. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Zhang, Junqin
Huang, Yunzhen
Li, Linlin
Dong, Jiawen
Kuang, Ruihuan
Liao, Ming
Sun, Minhua
First Identification and Genetic Characterization of a Novel Duck Astrovirus in Ducklings in China
title First Identification and Genetic Characterization of a Novel Duck Astrovirus in Ducklings in China
title_full First Identification and Genetic Characterization of a Novel Duck Astrovirus in Ducklings in China
title_fullStr First Identification and Genetic Characterization of a Novel Duck Astrovirus in Ducklings in China
title_full_unstemmed First Identification and Genetic Characterization of a Novel Duck Astrovirus in Ducklings in China
title_short First Identification and Genetic Characterization of a Novel Duck Astrovirus in Ducklings in China
title_sort first identification and genetic characterization of a novel duck astrovirus in ducklings in china
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9024104/
https://www.ncbi.nlm.nih.gov/pubmed/35464380
http://dx.doi.org/10.3389/fvets.2022.873062
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