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Vaginal Microbiota Diversity in Response to Lipopolysaccharide in Gilts Housed Under Three Housing Systems

The United Kingdom and European Union have banned crates for pregnant sows. However, animals are kept in a restrictive environment for up to four weeks after mating, leading to stress and different responses of the animals’ immune system. Here, we used vaginal flushing of gilts to investigate whethe...

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Autores principales: Alves, Luana, de Novais, Francisco José, da Silva, Arthur Nery, Araujo, Michelle Silva, Bernardino, Thiago, Osowski, Germana Vizzotto, Zanella, Ricardo, Lee Settles, Matthew, Holmes, Mark A., Fukumasu, Heidge, Ruiz, Vera Letticie de Azevedo, Zanella, Adroaldo José
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9024362/
https://www.ncbi.nlm.nih.gov/pubmed/35464863
http://dx.doi.org/10.3389/fgene.2022.836962
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author Alves, Luana
de Novais, Francisco José
da Silva, Arthur Nery
Araujo, Michelle Silva
Bernardino, Thiago
Osowski, Germana Vizzotto
Zanella, Ricardo
Lee Settles, Matthew
Holmes, Mark A.
Fukumasu, Heidge
Ruiz, Vera Letticie de Azevedo
Zanella, Adroaldo José
author_facet Alves, Luana
de Novais, Francisco José
da Silva, Arthur Nery
Araujo, Michelle Silva
Bernardino, Thiago
Osowski, Germana Vizzotto
Zanella, Ricardo
Lee Settles, Matthew
Holmes, Mark A.
Fukumasu, Heidge
Ruiz, Vera Letticie de Azevedo
Zanella, Adroaldo José
author_sort Alves, Luana
collection PubMed
description The United Kingdom and European Union have banned crates for pregnant sows. However, animals are kept in a restrictive environment for up to four weeks after mating, leading to stress and different responses of the animals’ immune system. Here, we used vaginal flushing of gilts to investigate whether housing systems or an experimental inflammatory challenge with lipopolysaccharide (LPS) can modify the gilt vaginal microbiome. Alpha-diversity indices showed differences in the microbiota of gilts housed under different systems (q = 0.04). Shannon alpha-diversity richness was higher in gilts group-housed in pens than in gilts housed in crates (q = 0.035), but not higher than in other groups. The relative abundance of the operational taxonomic unit (OTU) (q < 0.05) revealed specific differences in housing systems before a LPS or saline (SAL control) challenge. We found different abundances in taxa of Actinobacteria, Bacteroidetes, Cyanobacteria, Firmicutes, and Proteobacteria in gilts housed in the different systems before challenge. After the LPS challenge, significant differences were detected in the relative abundance of OTUs (q < 0.05) for the LPS-challenged group compared with SAL animals for each housing system. The phylum Staphylococcus showed higher abundance among the LPS-challenged gilts than in SAL-challenged animals. Furthermore, Enterobacter was more abundant in the LPS-challenged gilts housed in crates than in SAL-challenged gilts housed in crates. Streptococcus suis, Conchiformibius, Globicatella and Actinobacillus were more abundant in LPS-challenged gilts in indoor group housing than in SAL gilts in the same housing system. Gilts kept outdoors did not show changes in vaginal microbiota after an LPS challenge. Gilts housed in crates showed clinical signs of urogenital infection, whereas gilts housed outdoors and in indoor group housing did not. The relationship between environment, immune response, and microbiota suggested that animals in a poor environments experience difficulties responding to a challenge and their vaginal microbiota is altered as a consequence, with decreased richness of normal vaginal microbiota, and increased opportunistic bacteria. Welfare indicators measured by gilts’ responses to housing systems however, do not fully explain mechanisms associated with the unique signature in vaginal microbiota encountered in the different housing systems.
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spelling pubmed-90243622022-04-23 Vaginal Microbiota Diversity in Response to Lipopolysaccharide in Gilts Housed Under Three Housing Systems Alves, Luana de Novais, Francisco José da Silva, Arthur Nery Araujo, Michelle Silva Bernardino, Thiago Osowski, Germana Vizzotto Zanella, Ricardo Lee Settles, Matthew Holmes, Mark A. Fukumasu, Heidge Ruiz, Vera Letticie de Azevedo Zanella, Adroaldo José Front Genet Genetics The United Kingdom and European Union have banned crates for pregnant sows. However, animals are kept in a restrictive environment for up to four weeks after mating, leading to stress and different responses of the animals’ immune system. Here, we used vaginal flushing of gilts to investigate whether housing systems or an experimental inflammatory challenge with lipopolysaccharide (LPS) can modify the gilt vaginal microbiome. Alpha-diversity indices showed differences in the microbiota of gilts housed under different systems (q = 0.04). Shannon alpha-diversity richness was higher in gilts group-housed in pens than in gilts housed in crates (q = 0.035), but not higher than in other groups. The relative abundance of the operational taxonomic unit (OTU) (q < 0.05) revealed specific differences in housing systems before a LPS or saline (SAL control) challenge. We found different abundances in taxa of Actinobacteria, Bacteroidetes, Cyanobacteria, Firmicutes, and Proteobacteria in gilts housed in the different systems before challenge. After the LPS challenge, significant differences were detected in the relative abundance of OTUs (q < 0.05) for the LPS-challenged group compared with SAL animals for each housing system. The phylum Staphylococcus showed higher abundance among the LPS-challenged gilts than in SAL-challenged animals. Furthermore, Enterobacter was more abundant in the LPS-challenged gilts housed in crates than in SAL-challenged gilts housed in crates. Streptococcus suis, Conchiformibius, Globicatella and Actinobacillus were more abundant in LPS-challenged gilts in indoor group housing than in SAL gilts in the same housing system. Gilts kept outdoors did not show changes in vaginal microbiota after an LPS challenge. Gilts housed in crates showed clinical signs of urogenital infection, whereas gilts housed outdoors and in indoor group housing did not. The relationship between environment, immune response, and microbiota suggested that animals in a poor environments experience difficulties responding to a challenge and their vaginal microbiota is altered as a consequence, with decreased richness of normal vaginal microbiota, and increased opportunistic bacteria. Welfare indicators measured by gilts’ responses to housing systems however, do not fully explain mechanisms associated with the unique signature in vaginal microbiota encountered in the different housing systems. Frontiers Media S.A. 2022-04-08 /pmc/articles/PMC9024362/ /pubmed/35464863 http://dx.doi.org/10.3389/fgene.2022.836962 Text en Copyright © 2022 Alves, de Novais, da Silva, Araujo, Bernardino, Osowski, Zanella, Lee Settles, Holmes, Fukumasu, Ruiz and Zanella. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Alves, Luana
de Novais, Francisco José
da Silva, Arthur Nery
Araujo, Michelle Silva
Bernardino, Thiago
Osowski, Germana Vizzotto
Zanella, Ricardo
Lee Settles, Matthew
Holmes, Mark A.
Fukumasu, Heidge
Ruiz, Vera Letticie de Azevedo
Zanella, Adroaldo José
Vaginal Microbiota Diversity in Response to Lipopolysaccharide in Gilts Housed Under Three Housing Systems
title Vaginal Microbiota Diversity in Response to Lipopolysaccharide in Gilts Housed Under Three Housing Systems
title_full Vaginal Microbiota Diversity in Response to Lipopolysaccharide in Gilts Housed Under Three Housing Systems
title_fullStr Vaginal Microbiota Diversity in Response to Lipopolysaccharide in Gilts Housed Under Three Housing Systems
title_full_unstemmed Vaginal Microbiota Diversity in Response to Lipopolysaccharide in Gilts Housed Under Three Housing Systems
title_short Vaginal Microbiota Diversity in Response to Lipopolysaccharide in Gilts Housed Under Three Housing Systems
title_sort vaginal microbiota diversity in response to lipopolysaccharide in gilts housed under three housing systems
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9024362/
https://www.ncbi.nlm.nih.gov/pubmed/35464863
http://dx.doi.org/10.3389/fgene.2022.836962
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