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Changes in the Gut Metabolic Profile of Gestational Diabetes Mellitus Rats Following Probiotic Supplementation

The roles of gut microbiota and metabolomics in women with gestational diabetes mellitus (GDM) are not well understood. This study investigated the gut metabolomic profiling of GDM rats and GDM rats treated with probiotic supplements. Associations between gut metabolites and microbiota were also stu...

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Autores principales: Zheng, Qing-Xiang, Wang, Hai-Wei, Jiang, Xiu-Min, Ge, Li, Lai, Yu-Ting, Jiang, Xin-Yong, Huang, Ping-Ping, Chen, Fan, Chen, Xiao-Qian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9024396/
https://www.ncbi.nlm.nih.gov/pubmed/35464990
http://dx.doi.org/10.3389/fmicb.2022.779314
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author Zheng, Qing-Xiang
Wang, Hai-Wei
Jiang, Xiu-Min
Ge, Li
Lai, Yu-Ting
Jiang, Xin-Yong
Huang, Ping-Ping
Chen, Fan
Chen, Xiao-Qian
author_facet Zheng, Qing-Xiang
Wang, Hai-Wei
Jiang, Xiu-Min
Ge, Li
Lai, Yu-Ting
Jiang, Xin-Yong
Huang, Ping-Ping
Chen, Fan
Chen, Xiao-Qian
author_sort Zheng, Qing-Xiang
collection PubMed
description The roles of gut microbiota and metabolomics in women with gestational diabetes mellitus (GDM) are not well understood. This study investigated the gut metabolomic profiling of GDM rats and GDM rats treated with probiotic supplements. Associations between gut metabolites and microbiota were also studied in GDM rats. Liquid chromatography–mass spectrometry was used to detect gut metabolites in GDM rats and GDM rats treated with probiotic supplements of 0.5 g (low-dose group) or 1 g (high-dose group) for 15 days. Each gram of probiotic supplement contained 5 × 10(7) colony-forming units (CFU) of Lactobacillus rhamnosus LGG and 1 × 10(8) CFU of Bifidobacterium animalis subspecies lactis Bb12. The association between gut metabolites and microbiota in GDM rats was investigated using Spearman’s correlation. Finally, 10 rats in the normal pregnant group, eight rats in the GDM model group, eight GDM rats in the low-dose probiotics group, and nine GDM rats in the high-dose probiotics group were further studied. Serum parameters and pancreatic and colon histology were significantly changed in GDM rats, and these were restored using probiotic supplements. In total, 999 gut metabolites were detected in the feces, and GDM rats were distinguished from normal rats. The levels of 44 metabolites were increased in GDM rats, and they were alleviated using probiotic supplements. Changes in metabolites in GDM rats were associated with amino acids and bile acids metabolism signaling pathways. Furthermore, changes in metabolites after probiotic supplementation were associated with porphyrin and chlorophyll metabolism pathways. We found that the Allobaculum genus displayed strong positive correlations, whereas the Bryobacter and Gemmatimonas genera displayed strong negative correlations with metabolisms of amino acids and bile acids in GDM rats. The Lactobacillus and Bifidobacterium genera were positively correlated with gut metabolites. Overall, our results showed that metabolism signaling pathways of amino acids and bile acids are associated with the development of GDM. Probiotic supplements alleviate the pathology of GDM through the metabolism pathways of amino acids, bile acids, porphyrin, and chlorophyll.
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spelling pubmed-90243962022-04-23 Changes in the Gut Metabolic Profile of Gestational Diabetes Mellitus Rats Following Probiotic Supplementation Zheng, Qing-Xiang Wang, Hai-Wei Jiang, Xiu-Min Ge, Li Lai, Yu-Ting Jiang, Xin-Yong Huang, Ping-Ping Chen, Fan Chen, Xiao-Qian Front Microbiol Microbiology The roles of gut microbiota and metabolomics in women with gestational diabetes mellitus (GDM) are not well understood. This study investigated the gut metabolomic profiling of GDM rats and GDM rats treated with probiotic supplements. Associations between gut metabolites and microbiota were also studied in GDM rats. Liquid chromatography–mass spectrometry was used to detect gut metabolites in GDM rats and GDM rats treated with probiotic supplements of 0.5 g (low-dose group) or 1 g (high-dose group) for 15 days. Each gram of probiotic supplement contained 5 × 10(7) colony-forming units (CFU) of Lactobacillus rhamnosus LGG and 1 × 10(8) CFU of Bifidobacterium animalis subspecies lactis Bb12. The association between gut metabolites and microbiota in GDM rats was investigated using Spearman’s correlation. Finally, 10 rats in the normal pregnant group, eight rats in the GDM model group, eight GDM rats in the low-dose probiotics group, and nine GDM rats in the high-dose probiotics group were further studied. Serum parameters and pancreatic and colon histology were significantly changed in GDM rats, and these were restored using probiotic supplements. In total, 999 gut metabolites were detected in the feces, and GDM rats were distinguished from normal rats. The levels of 44 metabolites were increased in GDM rats, and they were alleviated using probiotic supplements. Changes in metabolites in GDM rats were associated with amino acids and bile acids metabolism signaling pathways. Furthermore, changes in metabolites after probiotic supplementation were associated with porphyrin and chlorophyll metabolism pathways. We found that the Allobaculum genus displayed strong positive correlations, whereas the Bryobacter and Gemmatimonas genera displayed strong negative correlations with metabolisms of amino acids and bile acids in GDM rats. The Lactobacillus and Bifidobacterium genera were positively correlated with gut metabolites. Overall, our results showed that metabolism signaling pathways of amino acids and bile acids are associated with the development of GDM. Probiotic supplements alleviate the pathology of GDM through the metabolism pathways of amino acids, bile acids, porphyrin, and chlorophyll. Frontiers Media S.A. 2022-04-08 /pmc/articles/PMC9024396/ /pubmed/35464990 http://dx.doi.org/10.3389/fmicb.2022.779314 Text en Copyright © 2022 Zheng, Wang, Jiang, Ge, Lai, Jiang, Huang, Chen and Chen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Zheng, Qing-Xiang
Wang, Hai-Wei
Jiang, Xiu-Min
Ge, Li
Lai, Yu-Ting
Jiang, Xin-Yong
Huang, Ping-Ping
Chen, Fan
Chen, Xiao-Qian
Changes in the Gut Metabolic Profile of Gestational Diabetes Mellitus Rats Following Probiotic Supplementation
title Changes in the Gut Metabolic Profile of Gestational Diabetes Mellitus Rats Following Probiotic Supplementation
title_full Changes in the Gut Metabolic Profile of Gestational Diabetes Mellitus Rats Following Probiotic Supplementation
title_fullStr Changes in the Gut Metabolic Profile of Gestational Diabetes Mellitus Rats Following Probiotic Supplementation
title_full_unstemmed Changes in the Gut Metabolic Profile of Gestational Diabetes Mellitus Rats Following Probiotic Supplementation
title_short Changes in the Gut Metabolic Profile of Gestational Diabetes Mellitus Rats Following Probiotic Supplementation
title_sort changes in the gut metabolic profile of gestational diabetes mellitus rats following probiotic supplementation
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9024396/
https://www.ncbi.nlm.nih.gov/pubmed/35464990
http://dx.doi.org/10.3389/fmicb.2022.779314
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