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Co-Expression Networks Unveiled Long Non-Coding RNAs as Molecular Targets of Drugs Used to Treat Bipolar Disorder
Long non-coding RNAs (lncRNAs) may play a role in psychiatric diseases including bipolar disorder (BD). We investigated mRNA-lncRNA co-expression patterns in neuronal-like cells treated with widely prescribed BD medications. The aim was to unveil insights into the complex mechanisms of BD medication...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9024411/ https://www.ncbi.nlm.nih.gov/pubmed/35462908 http://dx.doi.org/10.3389/fphar.2022.873271 |
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author | Truong, Trang TT. Bortolasci, Chiara C. Spolding, Briana Panizzutti, Bruna Liu, Zoe SJ. Kidnapillai, Srisaiyini Richardson, Mark Gray, Laura Smith, Craig M. Dean, Olivia M. Kim, Jee Hyun Berk, Michael Walder, Ken |
author_facet | Truong, Trang TT. Bortolasci, Chiara C. Spolding, Briana Panizzutti, Bruna Liu, Zoe SJ. Kidnapillai, Srisaiyini Richardson, Mark Gray, Laura Smith, Craig M. Dean, Olivia M. Kim, Jee Hyun Berk, Michael Walder, Ken |
author_sort | Truong, Trang TT. |
collection | PubMed |
description | Long non-coding RNAs (lncRNAs) may play a role in psychiatric diseases including bipolar disorder (BD). We investigated mRNA-lncRNA co-expression patterns in neuronal-like cells treated with widely prescribed BD medications. The aim was to unveil insights into the complex mechanisms of BD medications and highlight potential targets for new drug development. Human neuronal-like (NT2-N) cells were treated with either lamotrigine, lithium, quetiapine, valproate or vehicle for 24 h. Genome-wide mRNA expression was quantified for weighted gene co-expression network analysis (WGCNA) to correlate the expression levels of mRNAs with lncRNAs. Functional enrichment analysis and hub lncRNA identification was conducted on key co-expressed modules associated with the drug response. We constructed lncRNA-mRNA co-expression networks and identified key modules underlying these treatments, as well as their enriched biological functions. Processes enriched in key modules included synaptic vesicle cycle, endoplasmic reticulum-related functions and neurodevelopment. Several lncRNAs such as GAS6-AS1 and MIR100HG were highlighted as driver genes of key modules. Our study demonstrates the key role of lncRNAs in the mechanism(s) of action of BD drugs. Several lncRNAs have been suggested as major regulators of medication effects and are worthy of further investigation as novel drug targets to treat BD. |
format | Online Article Text |
id | pubmed-9024411 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-90244112022-04-23 Co-Expression Networks Unveiled Long Non-Coding RNAs as Molecular Targets of Drugs Used to Treat Bipolar Disorder Truong, Trang TT. Bortolasci, Chiara C. Spolding, Briana Panizzutti, Bruna Liu, Zoe SJ. Kidnapillai, Srisaiyini Richardson, Mark Gray, Laura Smith, Craig M. Dean, Olivia M. Kim, Jee Hyun Berk, Michael Walder, Ken Front Pharmacol Pharmacology Long non-coding RNAs (lncRNAs) may play a role in psychiatric diseases including bipolar disorder (BD). We investigated mRNA-lncRNA co-expression patterns in neuronal-like cells treated with widely prescribed BD medications. The aim was to unveil insights into the complex mechanisms of BD medications and highlight potential targets for new drug development. Human neuronal-like (NT2-N) cells were treated with either lamotrigine, lithium, quetiapine, valproate or vehicle for 24 h. Genome-wide mRNA expression was quantified for weighted gene co-expression network analysis (WGCNA) to correlate the expression levels of mRNAs with lncRNAs. Functional enrichment analysis and hub lncRNA identification was conducted on key co-expressed modules associated with the drug response. We constructed lncRNA-mRNA co-expression networks and identified key modules underlying these treatments, as well as their enriched biological functions. Processes enriched in key modules included synaptic vesicle cycle, endoplasmic reticulum-related functions and neurodevelopment. Several lncRNAs such as GAS6-AS1 and MIR100HG were highlighted as driver genes of key modules. Our study demonstrates the key role of lncRNAs in the mechanism(s) of action of BD drugs. Several lncRNAs have been suggested as major regulators of medication effects and are worthy of further investigation as novel drug targets to treat BD. Frontiers Media S.A. 2022-04-08 /pmc/articles/PMC9024411/ /pubmed/35462908 http://dx.doi.org/10.3389/fphar.2022.873271 Text en Copyright © 2022 Truong, Bortolasci, Spolding, Panizzutti, Liu, Kidnapillai, Richardson, Gray, Smith, Dean, Kim, Berk and Walder. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Pharmacology Truong, Trang TT. Bortolasci, Chiara C. Spolding, Briana Panizzutti, Bruna Liu, Zoe SJ. Kidnapillai, Srisaiyini Richardson, Mark Gray, Laura Smith, Craig M. Dean, Olivia M. Kim, Jee Hyun Berk, Michael Walder, Ken Co-Expression Networks Unveiled Long Non-Coding RNAs as Molecular Targets of Drugs Used to Treat Bipolar Disorder |
title | Co-Expression Networks Unveiled Long Non-Coding RNAs as Molecular Targets of Drugs Used to Treat Bipolar Disorder |
title_full | Co-Expression Networks Unveiled Long Non-Coding RNAs as Molecular Targets of Drugs Used to Treat Bipolar Disorder |
title_fullStr | Co-Expression Networks Unveiled Long Non-Coding RNAs as Molecular Targets of Drugs Used to Treat Bipolar Disorder |
title_full_unstemmed | Co-Expression Networks Unveiled Long Non-Coding RNAs as Molecular Targets of Drugs Used to Treat Bipolar Disorder |
title_short | Co-Expression Networks Unveiled Long Non-Coding RNAs as Molecular Targets of Drugs Used to Treat Bipolar Disorder |
title_sort | co-expression networks unveiled long non-coding rnas as molecular targets of drugs used to treat bipolar disorder |
topic | Pharmacology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9024411/ https://www.ncbi.nlm.nih.gov/pubmed/35462908 http://dx.doi.org/10.3389/fphar.2022.873271 |
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