Significance of Lipid Fatty Acid Composition for Resistance to Winter Conditions in Asplenium scolopendrium

SIMPLE SUMMARY: Plants growing at temperate and polar latitudes are exposed to cold stress. With climate change, different durations of low temperatures and sometimes frost are being increasingly observed in regions at low latitudes. This can cause especially great harm to agricultural crops, the mortality of which can pose a serious challenge to the food supply for the global population. One of the factors affecting plant resistance to low and negative temperatures is specific changes in the fatty acid (FA) composition of lipids. It should be noted that most of the crops studied in this regard are angiosperms. It is known that the FA composition of angiosperms has undergone significant evolutionary changes compared to that of nonflowering vascular plants. Studying the FA composition of various taxonomic groups can shed light on and reveal new mechanisms of plant resistance. Therefore, in this paper, we focused on the rare evergreen fern Asplenium scolopendrium, whose fronds can tolerate freezing. A number of specific features of its FA composition were discovered, which, in combination with other resistance mechanisms, determine its ability to grow in temperate climate zones and safely undergo wintering. ABSTRACT: Ferns are one of the oldest land plants. Among them, there are species that, during the course of evolution, have adapted to living in temperate climates and under winter conditions. Asplenium scolopendrium is one such species whose fronds are able to tolerate low subzero temperatures in winter. It is known that the resistance of ferns to freezing is associated with their prevention of desiccation via unique properties of the xylem and effective photoprotective mechanisms. In this work, the composition of A. scolopendrium lipid fatty acids (FAs) at different times of the year was studied by gas–liquid chromatography with mass spectrometry to determine their role in the resistance of this species to low temperatures. During the growing season, the polyunsaturated FA content increased significantly. This led to increases in the unsaturation and double-bond indices by winter. In addition, after emergence from snow, medium-chain FAs were found in the fronds. Thus, it can be speculated that the FA composition plays an important role in the adaptation of A. scolopendrium to growing conditions and preparation for successful wintering.