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Real-Time Detection of LAMP Products of African Swine Fever Virus Using Fluorescence and Surface Plasmon Resonance Method

African swine fever (ASF) is a swine disease with a very high fatality rate caused by a complex double-stranded DNA virus. The fluorescence PCR detection method is widely used for virus nucleic acid detection. Surface plasmon resonance (SPR) is a label-free and real-time detection method, unlike the...

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Autores principales: Zhang, Hao, Yao, Yuan, Chen, Zhi, Sun, Wenbo, Liu, Xiang, Chen, Lei, Sun, Jianhai, Qiu, Xianbo, Yu, Duli, Zhang, Lulu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9024557/
https://www.ncbi.nlm.nih.gov/pubmed/35448273
http://dx.doi.org/10.3390/bios12040213
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author Zhang, Hao
Yao, Yuan
Chen, Zhi
Sun, Wenbo
Liu, Xiang
Chen, Lei
Sun, Jianhai
Qiu, Xianbo
Yu, Duli
Zhang, Lulu
author_facet Zhang, Hao
Yao, Yuan
Chen, Zhi
Sun, Wenbo
Liu, Xiang
Chen, Lei
Sun, Jianhai
Qiu, Xianbo
Yu, Duli
Zhang, Lulu
author_sort Zhang, Hao
collection PubMed
description African swine fever (ASF) is a swine disease with a very high fatality rate caused by a complex double-stranded DNA virus. The fluorescence PCR detection method is widely used for virus nucleic acid detection. Surface plasmon resonance (SPR) is a label-free and real-time detection method, unlike the fluorescence PCR detection method. In this research, we detected the loop-mediated isothermal amplification (LAMP) products of the African swine fever virus by using the SPR and fluorescence methods separately and simultaneously. By comparing the positive and negative control results, we found that the SPR response unit is completely different before and after the LAMP process. In addition, the fluorescence results on a chip showed that with an increase in the concentration of the sample, the cycle threshold (CT) value decreased, which is consistent with commercial instruments. Both the decline rate of the SPR response unit and the CT value of the fluorescence realized were used to distinguish the positive control from the negative control and water, which indicates that the SPR method can be combined with fluorescence to detect LAMP products. This research provides a label-free and simple method for detecting LAMP products.
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spelling pubmed-90245572022-04-23 Real-Time Detection of LAMP Products of African Swine Fever Virus Using Fluorescence and Surface Plasmon Resonance Method Zhang, Hao Yao, Yuan Chen, Zhi Sun, Wenbo Liu, Xiang Chen, Lei Sun, Jianhai Qiu, Xianbo Yu, Duli Zhang, Lulu Biosensors (Basel) Communication African swine fever (ASF) is a swine disease with a very high fatality rate caused by a complex double-stranded DNA virus. The fluorescence PCR detection method is widely used for virus nucleic acid detection. Surface plasmon resonance (SPR) is a label-free and real-time detection method, unlike the fluorescence PCR detection method. In this research, we detected the loop-mediated isothermal amplification (LAMP) products of the African swine fever virus by using the SPR and fluorescence methods separately and simultaneously. By comparing the positive and negative control results, we found that the SPR response unit is completely different before and after the LAMP process. In addition, the fluorescence results on a chip showed that with an increase in the concentration of the sample, the cycle threshold (CT) value decreased, which is consistent with commercial instruments. Both the decline rate of the SPR response unit and the CT value of the fluorescence realized were used to distinguish the positive control from the negative control and water, which indicates that the SPR method can be combined with fluorescence to detect LAMP products. This research provides a label-free and simple method for detecting LAMP products. MDPI 2022-04-03 /pmc/articles/PMC9024557/ /pubmed/35448273 http://dx.doi.org/10.3390/bios12040213 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Zhang, Hao
Yao, Yuan
Chen, Zhi
Sun, Wenbo
Liu, Xiang
Chen, Lei
Sun, Jianhai
Qiu, Xianbo
Yu, Duli
Zhang, Lulu
Real-Time Detection of LAMP Products of African Swine Fever Virus Using Fluorescence and Surface Plasmon Resonance Method
title Real-Time Detection of LAMP Products of African Swine Fever Virus Using Fluorescence and Surface Plasmon Resonance Method
title_full Real-Time Detection of LAMP Products of African Swine Fever Virus Using Fluorescence and Surface Plasmon Resonance Method
title_fullStr Real-Time Detection of LAMP Products of African Swine Fever Virus Using Fluorescence and Surface Plasmon Resonance Method
title_full_unstemmed Real-Time Detection of LAMP Products of African Swine Fever Virus Using Fluorescence and Surface Plasmon Resonance Method
title_short Real-Time Detection of LAMP Products of African Swine Fever Virus Using Fluorescence and Surface Plasmon Resonance Method
title_sort real-time detection of lamp products of african swine fever virus using fluorescence and surface plasmon resonance method
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9024557/
https://www.ncbi.nlm.nih.gov/pubmed/35448273
http://dx.doi.org/10.3390/bios12040213
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