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Real-Time Detection of LAMP Products of African Swine Fever Virus Using Fluorescence and Surface Plasmon Resonance Method
African swine fever (ASF) is a swine disease with a very high fatality rate caused by a complex double-stranded DNA virus. The fluorescence PCR detection method is widely used for virus nucleic acid detection. Surface plasmon resonance (SPR) is a label-free and real-time detection method, unlike the...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9024557/ https://www.ncbi.nlm.nih.gov/pubmed/35448273 http://dx.doi.org/10.3390/bios12040213 |
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author | Zhang, Hao Yao, Yuan Chen, Zhi Sun, Wenbo Liu, Xiang Chen, Lei Sun, Jianhai Qiu, Xianbo Yu, Duli Zhang, Lulu |
author_facet | Zhang, Hao Yao, Yuan Chen, Zhi Sun, Wenbo Liu, Xiang Chen, Lei Sun, Jianhai Qiu, Xianbo Yu, Duli Zhang, Lulu |
author_sort | Zhang, Hao |
collection | PubMed |
description | African swine fever (ASF) is a swine disease with a very high fatality rate caused by a complex double-stranded DNA virus. The fluorescence PCR detection method is widely used for virus nucleic acid detection. Surface plasmon resonance (SPR) is a label-free and real-time detection method, unlike the fluorescence PCR detection method. In this research, we detected the loop-mediated isothermal amplification (LAMP) products of the African swine fever virus by using the SPR and fluorescence methods separately and simultaneously. By comparing the positive and negative control results, we found that the SPR response unit is completely different before and after the LAMP process. In addition, the fluorescence results on a chip showed that with an increase in the concentration of the sample, the cycle threshold (CT) value decreased, which is consistent with commercial instruments. Both the decline rate of the SPR response unit and the CT value of the fluorescence realized were used to distinguish the positive control from the negative control and water, which indicates that the SPR method can be combined with fluorescence to detect LAMP products. This research provides a label-free and simple method for detecting LAMP products. |
format | Online Article Text |
id | pubmed-9024557 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-90245572022-04-23 Real-Time Detection of LAMP Products of African Swine Fever Virus Using Fluorescence and Surface Plasmon Resonance Method Zhang, Hao Yao, Yuan Chen, Zhi Sun, Wenbo Liu, Xiang Chen, Lei Sun, Jianhai Qiu, Xianbo Yu, Duli Zhang, Lulu Biosensors (Basel) Communication African swine fever (ASF) is a swine disease with a very high fatality rate caused by a complex double-stranded DNA virus. The fluorescence PCR detection method is widely used for virus nucleic acid detection. Surface plasmon resonance (SPR) is a label-free and real-time detection method, unlike the fluorescence PCR detection method. In this research, we detected the loop-mediated isothermal amplification (LAMP) products of the African swine fever virus by using the SPR and fluorescence methods separately and simultaneously. By comparing the positive and negative control results, we found that the SPR response unit is completely different before and after the LAMP process. In addition, the fluorescence results on a chip showed that with an increase in the concentration of the sample, the cycle threshold (CT) value decreased, which is consistent with commercial instruments. Both the decline rate of the SPR response unit and the CT value of the fluorescence realized were used to distinguish the positive control from the negative control and water, which indicates that the SPR method can be combined with fluorescence to detect LAMP products. This research provides a label-free and simple method for detecting LAMP products. MDPI 2022-04-03 /pmc/articles/PMC9024557/ /pubmed/35448273 http://dx.doi.org/10.3390/bios12040213 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Zhang, Hao Yao, Yuan Chen, Zhi Sun, Wenbo Liu, Xiang Chen, Lei Sun, Jianhai Qiu, Xianbo Yu, Duli Zhang, Lulu Real-Time Detection of LAMP Products of African Swine Fever Virus Using Fluorescence and Surface Plasmon Resonance Method |
title | Real-Time Detection of LAMP Products of African Swine Fever Virus Using Fluorescence and Surface Plasmon Resonance Method |
title_full | Real-Time Detection of LAMP Products of African Swine Fever Virus Using Fluorescence and Surface Plasmon Resonance Method |
title_fullStr | Real-Time Detection of LAMP Products of African Swine Fever Virus Using Fluorescence and Surface Plasmon Resonance Method |
title_full_unstemmed | Real-Time Detection of LAMP Products of African Swine Fever Virus Using Fluorescence and Surface Plasmon Resonance Method |
title_short | Real-Time Detection of LAMP Products of African Swine Fever Virus Using Fluorescence and Surface Plasmon Resonance Method |
title_sort | real-time detection of lamp products of african swine fever virus using fluorescence and surface plasmon resonance method |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9024557/ https://www.ncbi.nlm.nih.gov/pubmed/35448273 http://dx.doi.org/10.3390/bios12040213 |
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