Cargando…
Mitochondrial Generated Redox Stress Differently Affects the Endoplasmic Reticulum of Circulating Lymphocytes and Monocytes in Treatment-Naïve Hodgkin’s Lymphoma
Background. The redox stress caused by Hodgkin’s lymphoma (HL) also involves the peripheral blood mononucleated cells (PBMCs) even before chemotherapy. Here, we tested whether lymphocytes and monocytes show a different response to the increased mitochondrial generation of reactive oxygen species (RO...
Autores principales: | , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9024578/ https://www.ncbi.nlm.nih.gov/pubmed/35453447 http://dx.doi.org/10.3390/antiox11040762 |
_version_ | 1784690632978071552 |
---|---|
author | Marini, Cecilia Cossu, Vanessa Bauckneht, Matteo Carta, Sonia Lanfranchi, Francesco D’Amico, Francesca Ravera, Silvia Orengo, Anna Maria Ghiggi, Chiara Ballerini, Filippo Durando, Paolo Chiesa, Sabrina Miceli, Alberto Donegani, Maria Isabella Morbelli, Silvia Bruno, Silvia Sambuceti, Gianmario |
author_facet | Marini, Cecilia Cossu, Vanessa Bauckneht, Matteo Carta, Sonia Lanfranchi, Francesco D’Amico, Francesca Ravera, Silvia Orengo, Anna Maria Ghiggi, Chiara Ballerini, Filippo Durando, Paolo Chiesa, Sabrina Miceli, Alberto Donegani, Maria Isabella Morbelli, Silvia Bruno, Silvia Sambuceti, Gianmario |
author_sort | Marini, Cecilia |
collection | PubMed |
description | Background. The redox stress caused by Hodgkin’s lymphoma (HL) also involves the peripheral blood mononucleated cells (PBMCs) even before chemotherapy. Here, we tested whether lymphocytes and monocytes show a different response to the increased mitochondrial generation of reactive oxygen species (ROS). Methods. PBMCs, isolated from the blood of treatment-naïve HL patients and control subjects, underwent assessment of malondialdehyde content and enzymatic activity of both hexose- and glucose-6P dehydrogenase (H6PD and G6PD) as well as flow cytometric analysis of mitochondrial ROS content. These data were complemented by evaluating the uptake of the fluorescent glucose analogue 2-NBDG that is selectively stored within the endoplasmic reticulum (ER). Results. Malondialdehyde content was increased in the whole population of HL PBMCs. The oxidative damage matched an increased activity of G6PD, and even more of H6PD, that trigger the cytosolic and ER pentose phosphate pathways, respectively. At flow cytometry, the number of recovered viable cells was selectively decreased in HL lymphocytes that also showed a more pronounced increase in mitochondrial ROS generation and 2-NBDG uptake, with respect to monocytes. Conclusions. PBMCs of HL patients display a selective mitochondrial and ER redox stress most evident in lymphocytes already before the exposure to chemotherapy toxicity. |
format | Online Article Text |
id | pubmed-9024578 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-90245782022-04-23 Mitochondrial Generated Redox Stress Differently Affects the Endoplasmic Reticulum of Circulating Lymphocytes and Monocytes in Treatment-Naïve Hodgkin’s Lymphoma Marini, Cecilia Cossu, Vanessa Bauckneht, Matteo Carta, Sonia Lanfranchi, Francesco D’Amico, Francesca Ravera, Silvia Orengo, Anna Maria Ghiggi, Chiara Ballerini, Filippo Durando, Paolo Chiesa, Sabrina Miceli, Alberto Donegani, Maria Isabella Morbelli, Silvia Bruno, Silvia Sambuceti, Gianmario Antioxidants (Basel) Article Background. The redox stress caused by Hodgkin’s lymphoma (HL) also involves the peripheral blood mononucleated cells (PBMCs) even before chemotherapy. Here, we tested whether lymphocytes and monocytes show a different response to the increased mitochondrial generation of reactive oxygen species (ROS). Methods. PBMCs, isolated from the blood of treatment-naïve HL patients and control subjects, underwent assessment of malondialdehyde content and enzymatic activity of both hexose- and glucose-6P dehydrogenase (H6PD and G6PD) as well as flow cytometric analysis of mitochondrial ROS content. These data were complemented by evaluating the uptake of the fluorescent glucose analogue 2-NBDG that is selectively stored within the endoplasmic reticulum (ER). Results. Malondialdehyde content was increased in the whole population of HL PBMCs. The oxidative damage matched an increased activity of G6PD, and even more of H6PD, that trigger the cytosolic and ER pentose phosphate pathways, respectively. At flow cytometry, the number of recovered viable cells was selectively decreased in HL lymphocytes that also showed a more pronounced increase in mitochondrial ROS generation and 2-NBDG uptake, with respect to monocytes. Conclusions. PBMCs of HL patients display a selective mitochondrial and ER redox stress most evident in lymphocytes already before the exposure to chemotherapy toxicity. MDPI 2022-04-11 /pmc/articles/PMC9024578/ /pubmed/35453447 http://dx.doi.org/10.3390/antiox11040762 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Marini, Cecilia Cossu, Vanessa Bauckneht, Matteo Carta, Sonia Lanfranchi, Francesco D’Amico, Francesca Ravera, Silvia Orengo, Anna Maria Ghiggi, Chiara Ballerini, Filippo Durando, Paolo Chiesa, Sabrina Miceli, Alberto Donegani, Maria Isabella Morbelli, Silvia Bruno, Silvia Sambuceti, Gianmario Mitochondrial Generated Redox Stress Differently Affects the Endoplasmic Reticulum of Circulating Lymphocytes and Monocytes in Treatment-Naïve Hodgkin’s Lymphoma |
title | Mitochondrial Generated Redox Stress Differently Affects the Endoplasmic Reticulum of Circulating Lymphocytes and Monocytes in Treatment-Naïve Hodgkin’s Lymphoma |
title_full | Mitochondrial Generated Redox Stress Differently Affects the Endoplasmic Reticulum of Circulating Lymphocytes and Monocytes in Treatment-Naïve Hodgkin’s Lymphoma |
title_fullStr | Mitochondrial Generated Redox Stress Differently Affects the Endoplasmic Reticulum of Circulating Lymphocytes and Monocytes in Treatment-Naïve Hodgkin’s Lymphoma |
title_full_unstemmed | Mitochondrial Generated Redox Stress Differently Affects the Endoplasmic Reticulum of Circulating Lymphocytes and Monocytes in Treatment-Naïve Hodgkin’s Lymphoma |
title_short | Mitochondrial Generated Redox Stress Differently Affects the Endoplasmic Reticulum of Circulating Lymphocytes and Monocytes in Treatment-Naïve Hodgkin’s Lymphoma |
title_sort | mitochondrial generated redox stress differently affects the endoplasmic reticulum of circulating lymphocytes and monocytes in treatment-naïve hodgkin’s lymphoma |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9024578/ https://www.ncbi.nlm.nih.gov/pubmed/35453447 http://dx.doi.org/10.3390/antiox11040762 |
work_keys_str_mv | AT marinicecilia mitochondrialgeneratedredoxstressdifferentlyaffectstheendoplasmicreticulumofcirculatinglymphocytesandmonocytesintreatmentnaivehodgkinslymphoma AT cossuvanessa mitochondrialgeneratedredoxstressdifferentlyaffectstheendoplasmicreticulumofcirculatinglymphocytesandmonocytesintreatmentnaivehodgkinslymphoma AT baucknehtmatteo mitochondrialgeneratedredoxstressdifferentlyaffectstheendoplasmicreticulumofcirculatinglymphocytesandmonocytesintreatmentnaivehodgkinslymphoma AT cartasonia mitochondrialgeneratedredoxstressdifferentlyaffectstheendoplasmicreticulumofcirculatinglymphocytesandmonocytesintreatmentnaivehodgkinslymphoma AT lanfranchifrancesco mitochondrialgeneratedredoxstressdifferentlyaffectstheendoplasmicreticulumofcirculatinglymphocytesandmonocytesintreatmentnaivehodgkinslymphoma AT damicofrancesca mitochondrialgeneratedredoxstressdifferentlyaffectstheendoplasmicreticulumofcirculatinglymphocytesandmonocytesintreatmentnaivehodgkinslymphoma AT raverasilvia mitochondrialgeneratedredoxstressdifferentlyaffectstheendoplasmicreticulumofcirculatinglymphocytesandmonocytesintreatmentnaivehodgkinslymphoma AT orengoannamaria mitochondrialgeneratedredoxstressdifferentlyaffectstheendoplasmicreticulumofcirculatinglymphocytesandmonocytesintreatmentnaivehodgkinslymphoma AT ghiggichiara mitochondrialgeneratedredoxstressdifferentlyaffectstheendoplasmicreticulumofcirculatinglymphocytesandmonocytesintreatmentnaivehodgkinslymphoma AT ballerinifilippo mitochondrialgeneratedredoxstressdifferentlyaffectstheendoplasmicreticulumofcirculatinglymphocytesandmonocytesintreatmentnaivehodgkinslymphoma AT durandopaolo mitochondrialgeneratedredoxstressdifferentlyaffectstheendoplasmicreticulumofcirculatinglymphocytesandmonocytesintreatmentnaivehodgkinslymphoma AT chiesasabrina mitochondrialgeneratedredoxstressdifferentlyaffectstheendoplasmicreticulumofcirculatinglymphocytesandmonocytesintreatmentnaivehodgkinslymphoma AT micelialberto mitochondrialgeneratedredoxstressdifferentlyaffectstheendoplasmicreticulumofcirculatinglymphocytesandmonocytesintreatmentnaivehodgkinslymphoma AT doneganimariaisabella mitochondrialgeneratedredoxstressdifferentlyaffectstheendoplasmicreticulumofcirculatinglymphocytesandmonocytesintreatmentnaivehodgkinslymphoma AT morbellisilvia mitochondrialgeneratedredoxstressdifferentlyaffectstheendoplasmicreticulumofcirculatinglymphocytesandmonocytesintreatmentnaivehodgkinslymphoma AT brunosilvia mitochondrialgeneratedredoxstressdifferentlyaffectstheendoplasmicreticulumofcirculatinglymphocytesandmonocytesintreatmentnaivehodgkinslymphoma AT sambucetigianmario mitochondrialgeneratedredoxstressdifferentlyaffectstheendoplasmicreticulumofcirculatinglymphocytesandmonocytesintreatmentnaivehodgkinslymphoma |