ALV-miRNA-p19-01 Promotes Viral Replication via Targeting Dual Specificity Phosphatase 6

MicroRNAs (miRNAs) are a group of regulatory noncoding RNAs, serving as major regulators with a sequence-specific manner in multifarious biological processes. Although a series of viral families have been proved to encode miRNAs, few reports were available regarding the function of ALV-J-encoded miR...

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Autores principales: Yan, Yiming, Chen, Sheng, Liao, Liqin, Gao, Shuang, Pang, Yanling, Zhang, Xinheng, Zhang, Huanmin, Xie, Qingmei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9024826/
https://www.ncbi.nlm.nih.gov/pubmed/35458535
http://dx.doi.org/10.3390/v14040805
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author Yan, Yiming
Chen, Sheng
Liao, Liqin
Gao, Shuang
Pang, Yanling
Zhang, Xinheng
Zhang, Huanmin
Xie, Qingmei
author_facet Yan, Yiming
Chen, Sheng
Liao, Liqin
Gao, Shuang
Pang, Yanling
Zhang, Xinheng
Zhang, Huanmin
Xie, Qingmei
author_sort Yan, Yiming
collection PubMed
description MicroRNAs (miRNAs) are a group of regulatory noncoding RNAs, serving as major regulators with a sequence-specific manner in multifarious biological processes. Although a series of viral families have been proved to encode miRNAs, few reports were available regarding the function of ALV-J-encoded miRNA. Here, we reported a novel miRNA (designated ALV-miRNA-p19-01) in ALV-J-infected DF-1 cells. We found that ALV-miRNA-p19-01 is encoded by the genome of the ALV-J SCAU1903 strain (located at nucleotides site 779 to 801) in a classic miRNA biogenesis manner. The transfection of DF-1 cells with ALV-miRNA-p19-01 enhanced ALV-J replication, while the blockage of ALV-miRNA-p19-01 suppressed ALV-J replication. Furthermore, our data showed that ALV-miRNA-p19-01 promotes ALV-J replication by directly targeting the cellular gene dual specificity phosphatase 6 through regulating ERK2 activity.
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spelling pubmed-90248262022-04-23 ALV-miRNA-p19-01 Promotes Viral Replication via Targeting Dual Specificity Phosphatase 6 Yan, Yiming Chen, Sheng Liao, Liqin Gao, Shuang Pang, Yanling Zhang, Xinheng Zhang, Huanmin Xie, Qingmei Viruses Article MicroRNAs (miRNAs) are a group of regulatory noncoding RNAs, serving as major regulators with a sequence-specific manner in multifarious biological processes. Although a series of viral families have been proved to encode miRNAs, few reports were available regarding the function of ALV-J-encoded miRNA. Here, we reported a novel miRNA (designated ALV-miRNA-p19-01) in ALV-J-infected DF-1 cells. We found that ALV-miRNA-p19-01 is encoded by the genome of the ALV-J SCAU1903 strain (located at nucleotides site 779 to 801) in a classic miRNA biogenesis manner. The transfection of DF-1 cells with ALV-miRNA-p19-01 enhanced ALV-J replication, while the blockage of ALV-miRNA-p19-01 suppressed ALV-J replication. Furthermore, our data showed that ALV-miRNA-p19-01 promotes ALV-J replication by directly targeting the cellular gene dual specificity phosphatase 6 through regulating ERK2 activity. MDPI 2022-04-13 /pmc/articles/PMC9024826/ /pubmed/35458535 http://dx.doi.org/10.3390/v14040805 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yan, Yiming
Chen, Sheng
Liao, Liqin
Gao, Shuang
Pang, Yanling
Zhang, Xinheng
Zhang, Huanmin
Xie, Qingmei
ALV-miRNA-p19-01 Promotes Viral Replication via Targeting Dual Specificity Phosphatase 6
title ALV-miRNA-p19-01 Promotes Viral Replication via Targeting Dual Specificity Phosphatase 6
title_full ALV-miRNA-p19-01 Promotes Viral Replication via Targeting Dual Specificity Phosphatase 6
title_fullStr ALV-miRNA-p19-01 Promotes Viral Replication via Targeting Dual Specificity Phosphatase 6
title_full_unstemmed ALV-miRNA-p19-01 Promotes Viral Replication via Targeting Dual Specificity Phosphatase 6
title_short ALV-miRNA-p19-01 Promotes Viral Replication via Targeting Dual Specificity Phosphatase 6
title_sort alv-mirna-p19-01 promotes viral replication via targeting dual specificity phosphatase 6
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9024826/
https://www.ncbi.nlm.nih.gov/pubmed/35458535
http://dx.doi.org/10.3390/v14040805
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