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Digital Microfluidic Mixing via Reciprocating Motions of Droplets Driven by Contact Charge Electrophoresis

Contact charge electrophoresis (CCEP) is an electrically controllable manipulation technique of conductive droplets and particles by charging and discharging when in contact with the electrode. Given its straightforward operation mechanism, low cost, and ease of system construction, it has gained tr...

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Detalles Bibliográficos
Autores principales: Kim, Jaewook, Kim, Taeyung, Ji, Inseo, Hong, Jiwoo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9025259/
https://www.ncbi.nlm.nih.gov/pubmed/35457899
http://dx.doi.org/10.3390/mi13040593
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author Kim, Jaewook
Kim, Taeyung
Ji, Inseo
Hong, Jiwoo
author_facet Kim, Jaewook
Kim, Taeyung
Ji, Inseo
Hong, Jiwoo
author_sort Kim, Jaewook
collection PubMed
description Contact charge electrophoresis (CCEP) is an electrically controllable manipulation technique of conductive droplets and particles by charging and discharging when in contact with the electrode. Given its straightforward operation mechanism, low cost, and ease of system construction, it has gained traction as a versatile and potential strategy for the realistic establishment of lab-on-a-chip (LOC) in various engineering applications. We present a CCEP-based digital microfluidics (DMF) platform with two parallel electrode modules comprising assembled conventional pin header sockets, allowing for efficient mixing through horizontal and vertical shaking via droplet reciprocating motions. The temporal chromic change caused by the chemical reaction between the pH indicator and base solutions within the shaking droplets is quantitatively analyzed under various CCEP actuation conditions to evaluate the mixing performance in shaking droplets by vertical and horizontal reciprocating motions on the DMF platform. Furthermore, mixing flow patterns within shaking droplets are successfully visualized by a high-speed camera system. The suggested techniques can mix samples and reagents rapidly and efficiently in droplet-based microreactors for DMF applications, such as biochemical analysis and medical diagnostics.
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spelling pubmed-90252592022-04-23 Digital Microfluidic Mixing via Reciprocating Motions of Droplets Driven by Contact Charge Electrophoresis Kim, Jaewook Kim, Taeyung Ji, Inseo Hong, Jiwoo Micromachines (Basel) Article Contact charge electrophoresis (CCEP) is an electrically controllable manipulation technique of conductive droplets and particles by charging and discharging when in contact with the electrode. Given its straightforward operation mechanism, low cost, and ease of system construction, it has gained traction as a versatile and potential strategy for the realistic establishment of lab-on-a-chip (LOC) in various engineering applications. We present a CCEP-based digital microfluidics (DMF) platform with two parallel electrode modules comprising assembled conventional pin header sockets, allowing for efficient mixing through horizontal and vertical shaking via droplet reciprocating motions. The temporal chromic change caused by the chemical reaction between the pH indicator and base solutions within the shaking droplets is quantitatively analyzed under various CCEP actuation conditions to evaluate the mixing performance in shaking droplets by vertical and horizontal reciprocating motions on the DMF platform. Furthermore, mixing flow patterns within shaking droplets are successfully visualized by a high-speed camera system. The suggested techniques can mix samples and reagents rapidly and efficiently in droplet-based microreactors for DMF applications, such as biochemical analysis and medical diagnostics. MDPI 2022-04-10 /pmc/articles/PMC9025259/ /pubmed/35457899 http://dx.doi.org/10.3390/mi13040593 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kim, Jaewook
Kim, Taeyung
Ji, Inseo
Hong, Jiwoo
Digital Microfluidic Mixing via Reciprocating Motions of Droplets Driven by Contact Charge Electrophoresis
title Digital Microfluidic Mixing via Reciprocating Motions of Droplets Driven by Contact Charge Electrophoresis
title_full Digital Microfluidic Mixing via Reciprocating Motions of Droplets Driven by Contact Charge Electrophoresis
title_fullStr Digital Microfluidic Mixing via Reciprocating Motions of Droplets Driven by Contact Charge Electrophoresis
title_full_unstemmed Digital Microfluidic Mixing via Reciprocating Motions of Droplets Driven by Contact Charge Electrophoresis
title_short Digital Microfluidic Mixing via Reciprocating Motions of Droplets Driven by Contact Charge Electrophoresis
title_sort digital microfluidic mixing via reciprocating motions of droplets driven by contact charge electrophoresis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9025259/
https://www.ncbi.nlm.nih.gov/pubmed/35457899
http://dx.doi.org/10.3390/mi13040593
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