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Antimicrobial Volatiles of the Insect Pathogen Metarhizium brunneum

Fungal volatile organic compounds (VOCs) represent promising candidates for biopesticide fumigants to control crop pests and pathogens. Herein, VOCs produced using three strains of the entomopathogenic fungus Metarhizium brunneum were identified via GC-MS and screened for antimicrobial activity. The...

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Autores principales: Hummadi, Esam Hamid, Cetin, Yarkin, Demirbek, Merve, Kardar, Nadeems M., Khan, Shazia, Coates, Christopher J., Eastwood, Daniel C., Dudley, Ed, Maffeis, Thierry, Loveridge, Joel, Butt, Tariq M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9025432/
https://www.ncbi.nlm.nih.gov/pubmed/35448558
http://dx.doi.org/10.3390/jof8040326
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author Hummadi, Esam Hamid
Cetin, Yarkin
Demirbek, Merve
Kardar, Nadeems M.
Khan, Shazia
Coates, Christopher J.
Eastwood, Daniel C.
Dudley, Ed
Maffeis, Thierry
Loveridge, Joel
Butt, Tariq M.
author_facet Hummadi, Esam Hamid
Cetin, Yarkin
Demirbek, Merve
Kardar, Nadeems M.
Khan, Shazia
Coates, Christopher J.
Eastwood, Daniel C.
Dudley, Ed
Maffeis, Thierry
Loveridge, Joel
Butt, Tariq M.
author_sort Hummadi, Esam Hamid
collection PubMed
description Fungal volatile organic compounds (VOCs) represent promising candidates for biopesticide fumigants to control crop pests and pathogens. Herein, VOCs produced using three strains of the entomopathogenic fungus Metarhizium brunneum were identified via GC-MS and screened for antimicrobial activity. The VOC profiles varied with fungal strain, development state (mycelium, spores) and culture conditions. Selected VOCs were screened against a range of rhizosphere and non-rhizosphere microbes, including three Gram-negative bacteria (Escherichia coli, Pantoea agglomerans, Pseudomonas aeruginosa), five Gram-positive bacteria (Micrococcus luteus, Staphylococcus aureus, Bacillus subtilis, B. megaterium, B. thuringiensis), two yeasts (Candida albicans, Candida glabrata) and three plant pathogenic fungi (Pythium ultimum, Botrytis cinerea, Fusarium graminearum). Microbes differed in their sensitivity to the test compounds, with 1-octen-3-ol and isovaleric acid showing broad-spectrum antimicrobial activity. Yeasts and bacteria were inhibited by the same VOCs. Cryo-SEM showed that both yeasts and bacteria underwent some form of “autolysis”, where all components of the cell, including the cell wall, disintegrated with little evidence of their presence in the clear, inhibition zone. The oomycete (P. ultimum) and ascomycete fungi (F. graminearum, B. cinerea) were sensitive to a wider range of VOCs than the bacteria, suggesting that eukaryotic microbes are the main competitors to M. brunneum in the rhizosphere. The ability to alter the VOC profile in response to nutritional cues may assist M. brunneum to survive among the roots of a wide range of plant species. Our VOC studies provided new insights as to how M. brunneum may protect plants from pathogenic microbes and correspondingly promote healthy growth.
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spelling pubmed-90254322022-04-23 Antimicrobial Volatiles of the Insect Pathogen Metarhizium brunneum Hummadi, Esam Hamid Cetin, Yarkin Demirbek, Merve Kardar, Nadeems M. Khan, Shazia Coates, Christopher J. Eastwood, Daniel C. Dudley, Ed Maffeis, Thierry Loveridge, Joel Butt, Tariq M. J Fungi (Basel) Article Fungal volatile organic compounds (VOCs) represent promising candidates for biopesticide fumigants to control crop pests and pathogens. Herein, VOCs produced using three strains of the entomopathogenic fungus Metarhizium brunneum were identified via GC-MS and screened for antimicrobial activity. The VOC profiles varied with fungal strain, development state (mycelium, spores) and culture conditions. Selected VOCs were screened against a range of rhizosphere and non-rhizosphere microbes, including three Gram-negative bacteria (Escherichia coli, Pantoea agglomerans, Pseudomonas aeruginosa), five Gram-positive bacteria (Micrococcus luteus, Staphylococcus aureus, Bacillus subtilis, B. megaterium, B. thuringiensis), two yeasts (Candida albicans, Candida glabrata) and three plant pathogenic fungi (Pythium ultimum, Botrytis cinerea, Fusarium graminearum). Microbes differed in their sensitivity to the test compounds, with 1-octen-3-ol and isovaleric acid showing broad-spectrum antimicrobial activity. Yeasts and bacteria were inhibited by the same VOCs. Cryo-SEM showed that both yeasts and bacteria underwent some form of “autolysis”, where all components of the cell, including the cell wall, disintegrated with little evidence of their presence in the clear, inhibition zone. The oomycete (P. ultimum) and ascomycete fungi (F. graminearum, B. cinerea) were sensitive to a wider range of VOCs than the bacteria, suggesting that eukaryotic microbes are the main competitors to M. brunneum in the rhizosphere. The ability to alter the VOC profile in response to nutritional cues may assist M. brunneum to survive among the roots of a wide range of plant species. Our VOC studies provided new insights as to how M. brunneum may protect plants from pathogenic microbes and correspondingly promote healthy growth. MDPI 2022-03-22 /pmc/articles/PMC9025432/ /pubmed/35448558 http://dx.doi.org/10.3390/jof8040326 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hummadi, Esam Hamid
Cetin, Yarkin
Demirbek, Merve
Kardar, Nadeems M.
Khan, Shazia
Coates, Christopher J.
Eastwood, Daniel C.
Dudley, Ed
Maffeis, Thierry
Loveridge, Joel
Butt, Tariq M.
Antimicrobial Volatiles of the Insect Pathogen Metarhizium brunneum
title Antimicrobial Volatiles of the Insect Pathogen Metarhizium brunneum
title_full Antimicrobial Volatiles of the Insect Pathogen Metarhizium brunneum
title_fullStr Antimicrobial Volatiles of the Insect Pathogen Metarhizium brunneum
title_full_unstemmed Antimicrobial Volatiles of the Insect Pathogen Metarhizium brunneum
title_short Antimicrobial Volatiles of the Insect Pathogen Metarhizium brunneum
title_sort antimicrobial volatiles of the insect pathogen metarhizium brunneum
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9025432/
https://www.ncbi.nlm.nih.gov/pubmed/35448558
http://dx.doi.org/10.3390/jof8040326
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