Cargando…
The Effect of Low-Processing Temperature on the Physicochemical and Mechanical Properties of Bovine Hydroxyapatite Bone Substitutes
Bovine bone grafts (BBX) require protein removal as part of the manufacturing process to reduce antigenicity and, in consequence, to be safely used in humans. Deproteinisation may have direct effects on the characteristics of the bone material and on in vivo material performance. This research aimed...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9025514/ https://www.ncbi.nlm.nih.gov/pubmed/35454491 http://dx.doi.org/10.3390/ma15082798 |
_version_ | 1784690891188862976 |
---|---|
author | Abdelmoneim, Dina Porter, Gemma Claire Coates, Dawn Elizabeth Duncan, Warwick John Waddell, John Neil Hammer, Niels Li, Kai Chun |
author_facet | Abdelmoneim, Dina Porter, Gemma Claire Coates, Dawn Elizabeth Duncan, Warwick John Waddell, John Neil Hammer, Niels Li, Kai Chun |
author_sort | Abdelmoneim, Dina |
collection | PubMed |
description | Bovine bone grafts (BBX) require protein removal as part of the manufacturing process to reduce antigenicity and, in consequence, to be safely used in humans. Deproteinisation may have direct effects on the characteristics of the bone material and on in vivo material performance. This research aimed to comprehensively study the physicochemical and mechanical properties of BBX processed at low deproteinisation processing temperatures. Cubes of bovine bone (8 mm(3)) were treated with temperatures between 100 °C and 220 °C at 30 °C intervals and with pressures ranging from 1.01 to 24.58 Bar. The samples were characterised topographically and mechanically using scanning electron microscopy (SEM), atomic force microscopy (AFM), and uniaxial bending tests. The organic content and the chemical composition were determined using thermogravimetric analysis (TGA) and Fourier-transform infrared spectroscopy (FTIR). X-ray diffraction (XRD) and FTIR were also used to quantitatively determine the specimen crystallinity. Increasing temperature/pressure was associated with decreasing protein levels and compressive strength and increasing surface irregularities and crystallinity. The findings suggest that low-temperature processed bone is likely to exhibit a rapid in vivo degradation rate. The deproteinisation temperature can be adjusted to tailor the graft properties for specific applications. |
format | Online Article Text |
id | pubmed-9025514 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-90255142022-04-23 The Effect of Low-Processing Temperature on the Physicochemical and Mechanical Properties of Bovine Hydroxyapatite Bone Substitutes Abdelmoneim, Dina Porter, Gemma Claire Coates, Dawn Elizabeth Duncan, Warwick John Waddell, John Neil Hammer, Niels Li, Kai Chun Materials (Basel) Article Bovine bone grafts (BBX) require protein removal as part of the manufacturing process to reduce antigenicity and, in consequence, to be safely used in humans. Deproteinisation may have direct effects on the characteristics of the bone material and on in vivo material performance. This research aimed to comprehensively study the physicochemical and mechanical properties of BBX processed at low deproteinisation processing temperatures. Cubes of bovine bone (8 mm(3)) were treated with temperatures between 100 °C and 220 °C at 30 °C intervals and with pressures ranging from 1.01 to 24.58 Bar. The samples were characterised topographically and mechanically using scanning electron microscopy (SEM), atomic force microscopy (AFM), and uniaxial bending tests. The organic content and the chemical composition were determined using thermogravimetric analysis (TGA) and Fourier-transform infrared spectroscopy (FTIR). X-ray diffraction (XRD) and FTIR were also used to quantitatively determine the specimen crystallinity. Increasing temperature/pressure was associated with decreasing protein levels and compressive strength and increasing surface irregularities and crystallinity. The findings suggest that low-temperature processed bone is likely to exhibit a rapid in vivo degradation rate. The deproteinisation temperature can be adjusted to tailor the graft properties for specific applications. MDPI 2022-04-11 /pmc/articles/PMC9025514/ /pubmed/35454491 http://dx.doi.org/10.3390/ma15082798 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Abdelmoneim, Dina Porter, Gemma Claire Coates, Dawn Elizabeth Duncan, Warwick John Waddell, John Neil Hammer, Niels Li, Kai Chun The Effect of Low-Processing Temperature on the Physicochemical and Mechanical Properties of Bovine Hydroxyapatite Bone Substitutes |
title | The Effect of Low-Processing Temperature on the Physicochemical and Mechanical Properties of Bovine Hydroxyapatite Bone Substitutes |
title_full | The Effect of Low-Processing Temperature on the Physicochemical and Mechanical Properties of Bovine Hydroxyapatite Bone Substitutes |
title_fullStr | The Effect of Low-Processing Temperature on the Physicochemical and Mechanical Properties of Bovine Hydroxyapatite Bone Substitutes |
title_full_unstemmed | The Effect of Low-Processing Temperature on the Physicochemical and Mechanical Properties of Bovine Hydroxyapatite Bone Substitutes |
title_short | The Effect of Low-Processing Temperature on the Physicochemical and Mechanical Properties of Bovine Hydroxyapatite Bone Substitutes |
title_sort | effect of low-processing temperature on the physicochemical and mechanical properties of bovine hydroxyapatite bone substitutes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9025514/ https://www.ncbi.nlm.nih.gov/pubmed/35454491 http://dx.doi.org/10.3390/ma15082798 |
work_keys_str_mv | AT abdelmoneimdina theeffectoflowprocessingtemperatureonthephysicochemicalandmechanicalpropertiesofbovinehydroxyapatitebonesubstitutes AT portergemmaclaire theeffectoflowprocessingtemperatureonthephysicochemicalandmechanicalpropertiesofbovinehydroxyapatitebonesubstitutes AT coatesdawnelizabeth theeffectoflowprocessingtemperatureonthephysicochemicalandmechanicalpropertiesofbovinehydroxyapatitebonesubstitutes AT duncanwarwickjohn theeffectoflowprocessingtemperatureonthephysicochemicalandmechanicalpropertiesofbovinehydroxyapatitebonesubstitutes AT waddelljohnneil theeffectoflowprocessingtemperatureonthephysicochemicalandmechanicalpropertiesofbovinehydroxyapatitebonesubstitutes AT hammerniels theeffectoflowprocessingtemperatureonthephysicochemicalandmechanicalpropertiesofbovinehydroxyapatitebonesubstitutes AT likaichun theeffectoflowprocessingtemperatureonthephysicochemicalandmechanicalpropertiesofbovinehydroxyapatitebonesubstitutes AT abdelmoneimdina effectoflowprocessingtemperatureonthephysicochemicalandmechanicalpropertiesofbovinehydroxyapatitebonesubstitutes AT portergemmaclaire effectoflowprocessingtemperatureonthephysicochemicalandmechanicalpropertiesofbovinehydroxyapatitebonesubstitutes AT coatesdawnelizabeth effectoflowprocessingtemperatureonthephysicochemicalandmechanicalpropertiesofbovinehydroxyapatitebonesubstitutes AT duncanwarwickjohn effectoflowprocessingtemperatureonthephysicochemicalandmechanicalpropertiesofbovinehydroxyapatitebonesubstitutes AT waddelljohnneil effectoflowprocessingtemperatureonthephysicochemicalandmechanicalpropertiesofbovinehydroxyapatitebonesubstitutes AT hammerniels effectoflowprocessingtemperatureonthephysicochemicalandmechanicalpropertiesofbovinehydroxyapatitebonesubstitutes AT likaichun effectoflowprocessingtemperatureonthephysicochemicalandmechanicalpropertiesofbovinehydroxyapatitebonesubstitutes |