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Chlorophyll-a Pigment Measurement of Spirulina in Algal Growth Monitoring Using Portable Pulsed LED Fluorescence Lidar System
Chlorophyll-a measurement is important in algal growth and water quality monitoring in natural waters. A portable pulsed LED fluorescence lidar system based on the preliminary algal organic matter and pigments excitation–emission matrix (EEM) of commercialized AZTEC Spirulina powder at varying conce...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9025811/ https://www.ncbi.nlm.nih.gov/pubmed/35458924 http://dx.doi.org/10.3390/s22082940 |
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author | Cadondon, Jumar G. Ong, Prane Mariel B. Vallar, Edgar A. Shiina, Tatsuo Galvez, Maria Cecilia D. |
author_facet | Cadondon, Jumar G. Ong, Prane Mariel B. Vallar, Edgar A. Shiina, Tatsuo Galvez, Maria Cecilia D. |
author_sort | Cadondon, Jumar G. |
collection | PubMed |
description | Chlorophyll-a measurement is important in algal growth and water quality monitoring in natural waters. A portable pulsed LED fluorescence lidar system based on the preliminary algal organic matter and pigments excitation–emission matrix (EEM) of commercialized AZTEC Spirulina powder at varying concentrations was developed. Fluorescence peaks from EEMs showed increasing intensity as the Spirulina concentration increases. Using this information, an LED fluorescence lidar with a wavelength of 385 nm, pulse width of 10 ns, and repetition frequency of 500 kHz was constructed for chlorophyll detection at 680 nm. Turbidity measurements were also conducted at 700 nm emission wavelength at the same excitation wavelength. Range-resolved fluorescence lidar signals from the portable pulsed LED fluorescence lidar system are highly correlated with the standard methods such as optical density at 680 nm (R(2) = 0.87), EEM fluorescence chlorophyll-a pigment at 680 nm (R(2) = 0.89), and corrected chlorophyll-a concentration (R(2) =0.92). The F680/F700 lidar ratio was measured to provide a linear relationship of chlorophyll-a and turbidity in waters. The F680/F700 measurement showed strong correlations with Spirulina concentration (R(2) = 0.94), absorbance at 680 nm (R(2) = 0.84), EEM chlorophyll-a pigment at 680 nm (R(2) = 0.83), and corrected chlorophyll-a concentration (R(2) = 0.86). Results revealed that this new technique of chlorophyll-a measurement can be used as an alternative to other standard methods in algal growth monitoring. |
format | Online Article Text |
id | pubmed-9025811 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-90258112022-04-23 Chlorophyll-a Pigment Measurement of Spirulina in Algal Growth Monitoring Using Portable Pulsed LED Fluorescence Lidar System Cadondon, Jumar G. Ong, Prane Mariel B. Vallar, Edgar A. Shiina, Tatsuo Galvez, Maria Cecilia D. Sensors (Basel) Article Chlorophyll-a measurement is important in algal growth and water quality monitoring in natural waters. A portable pulsed LED fluorescence lidar system based on the preliminary algal organic matter and pigments excitation–emission matrix (EEM) of commercialized AZTEC Spirulina powder at varying concentrations was developed. Fluorescence peaks from EEMs showed increasing intensity as the Spirulina concentration increases. Using this information, an LED fluorescence lidar with a wavelength of 385 nm, pulse width of 10 ns, and repetition frequency of 500 kHz was constructed for chlorophyll detection at 680 nm. Turbidity measurements were also conducted at 700 nm emission wavelength at the same excitation wavelength. Range-resolved fluorescence lidar signals from the portable pulsed LED fluorescence lidar system are highly correlated with the standard methods such as optical density at 680 nm (R(2) = 0.87), EEM fluorescence chlorophyll-a pigment at 680 nm (R(2) = 0.89), and corrected chlorophyll-a concentration (R(2) =0.92). The F680/F700 lidar ratio was measured to provide a linear relationship of chlorophyll-a and turbidity in waters. The F680/F700 measurement showed strong correlations with Spirulina concentration (R(2) = 0.94), absorbance at 680 nm (R(2) = 0.84), EEM chlorophyll-a pigment at 680 nm (R(2) = 0.83), and corrected chlorophyll-a concentration (R(2) = 0.86). Results revealed that this new technique of chlorophyll-a measurement can be used as an alternative to other standard methods in algal growth monitoring. MDPI 2022-04-12 /pmc/articles/PMC9025811/ /pubmed/35458924 http://dx.doi.org/10.3390/s22082940 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Cadondon, Jumar G. Ong, Prane Mariel B. Vallar, Edgar A. Shiina, Tatsuo Galvez, Maria Cecilia D. Chlorophyll-a Pigment Measurement of Spirulina in Algal Growth Monitoring Using Portable Pulsed LED Fluorescence Lidar System |
title | Chlorophyll-a Pigment Measurement of Spirulina in Algal Growth Monitoring Using Portable Pulsed LED Fluorescence Lidar System |
title_full | Chlorophyll-a Pigment Measurement of Spirulina in Algal Growth Monitoring Using Portable Pulsed LED Fluorescence Lidar System |
title_fullStr | Chlorophyll-a Pigment Measurement of Spirulina in Algal Growth Monitoring Using Portable Pulsed LED Fluorescence Lidar System |
title_full_unstemmed | Chlorophyll-a Pigment Measurement of Spirulina in Algal Growth Monitoring Using Portable Pulsed LED Fluorescence Lidar System |
title_short | Chlorophyll-a Pigment Measurement of Spirulina in Algal Growth Monitoring Using Portable Pulsed LED Fluorescence Lidar System |
title_sort | chlorophyll-a pigment measurement of spirulina in algal growth monitoring using portable pulsed led fluorescence lidar system |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9025811/ https://www.ncbi.nlm.nih.gov/pubmed/35458924 http://dx.doi.org/10.3390/s22082940 |
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