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Genome-Wide Analysis of the LRR-RLP Gene Family in a Wild Banana (Musa acuminata ssp. malaccensis) Uncovers Multiple Fusarium Wilt Resistance Gene Candidates

Banana is the most popular fruit in the world, with a relevant role in food security for more than 400 million people. However, fungal diseases cause substantial losses every year. A better understanding of the banana immune system should facilitate the development of new disease-resistant cultivars...

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Autores principales: Álvarez-López, Dulce, Herrera-Valencia, Virginia Aurora, Góngora-Castillo, Elsa, García-Laynes, Sergio, Puch-Hau, Carlos, López-Ochoa, Luisa Alhucema, Lizama-Uc, Gabriel, Peraza-Echeverria, Santy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9025879/
https://www.ncbi.nlm.nih.gov/pubmed/35456444
http://dx.doi.org/10.3390/genes13040638
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author Álvarez-López, Dulce
Herrera-Valencia, Virginia Aurora
Góngora-Castillo, Elsa
García-Laynes, Sergio
Puch-Hau, Carlos
López-Ochoa, Luisa Alhucema
Lizama-Uc, Gabriel
Peraza-Echeverria, Santy
author_facet Álvarez-López, Dulce
Herrera-Valencia, Virginia Aurora
Góngora-Castillo, Elsa
García-Laynes, Sergio
Puch-Hau, Carlos
López-Ochoa, Luisa Alhucema
Lizama-Uc, Gabriel
Peraza-Echeverria, Santy
author_sort Álvarez-López, Dulce
collection PubMed
description Banana is the most popular fruit in the world, with a relevant role in food security for more than 400 million people. However, fungal diseases cause substantial losses every year. A better understanding of the banana immune system should facilitate the development of new disease-resistant cultivars. In this study, we performed a genome-wide analysis of the leucine-rich repeat receptor-like protein (LRR-RLP) disease resistance gene family in a wild banana. We identified 78 LRR-RLP genes in the banana genome. Remarkably, seven MaLRR-RLPs formed a gene cluster in the distal part of chromosome 10, where resistance to Fusarium wilt caused by Foc race 1 has been previously mapped. Hence, we proposed these seven MaLRR-RLPs as resistance gene candidates (RGCs) for Fusarium wilt. We also identified seven other banana RGCs based on their close phylogenetic relationships with known LRR-RLP proteins. Moreover, phylogenetic analysis of the banana, rice, and Arabidopsis LRR-RLP families revealed five major phylogenetic clades shared by these plant species. Finally, transcriptomic analysis of the MaLRR-RLP gene family in plants treated with Foc race 1 or Foc TR4 showed the expression of several members of this family, and some of them were upregulated in response to these Foc races. Our study provides novel insights into the structure, distribution, evolution, and expression of the LRR-RLP gene family in bananas as well as valuable RGCs that will facilitate the identification of disease resistance genes for the genetic improvement of this crop.
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spelling pubmed-90258792022-04-23 Genome-Wide Analysis of the LRR-RLP Gene Family in a Wild Banana (Musa acuminata ssp. malaccensis) Uncovers Multiple Fusarium Wilt Resistance Gene Candidates Álvarez-López, Dulce Herrera-Valencia, Virginia Aurora Góngora-Castillo, Elsa García-Laynes, Sergio Puch-Hau, Carlos López-Ochoa, Luisa Alhucema Lizama-Uc, Gabriel Peraza-Echeverria, Santy Genes (Basel) Article Banana is the most popular fruit in the world, with a relevant role in food security for more than 400 million people. However, fungal diseases cause substantial losses every year. A better understanding of the banana immune system should facilitate the development of new disease-resistant cultivars. In this study, we performed a genome-wide analysis of the leucine-rich repeat receptor-like protein (LRR-RLP) disease resistance gene family in a wild banana. We identified 78 LRR-RLP genes in the banana genome. Remarkably, seven MaLRR-RLPs formed a gene cluster in the distal part of chromosome 10, where resistance to Fusarium wilt caused by Foc race 1 has been previously mapped. Hence, we proposed these seven MaLRR-RLPs as resistance gene candidates (RGCs) for Fusarium wilt. We also identified seven other banana RGCs based on their close phylogenetic relationships with known LRR-RLP proteins. Moreover, phylogenetic analysis of the banana, rice, and Arabidopsis LRR-RLP families revealed five major phylogenetic clades shared by these plant species. Finally, transcriptomic analysis of the MaLRR-RLP gene family in plants treated with Foc race 1 or Foc TR4 showed the expression of several members of this family, and some of them were upregulated in response to these Foc races. Our study provides novel insights into the structure, distribution, evolution, and expression of the LRR-RLP gene family in bananas as well as valuable RGCs that will facilitate the identification of disease resistance genes for the genetic improvement of this crop. MDPI 2022-04-02 /pmc/articles/PMC9025879/ /pubmed/35456444 http://dx.doi.org/10.3390/genes13040638 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Álvarez-López, Dulce
Herrera-Valencia, Virginia Aurora
Góngora-Castillo, Elsa
García-Laynes, Sergio
Puch-Hau, Carlos
López-Ochoa, Luisa Alhucema
Lizama-Uc, Gabriel
Peraza-Echeverria, Santy
Genome-Wide Analysis of the LRR-RLP Gene Family in a Wild Banana (Musa acuminata ssp. malaccensis) Uncovers Multiple Fusarium Wilt Resistance Gene Candidates
title Genome-Wide Analysis of the LRR-RLP Gene Family in a Wild Banana (Musa acuminata ssp. malaccensis) Uncovers Multiple Fusarium Wilt Resistance Gene Candidates
title_full Genome-Wide Analysis of the LRR-RLP Gene Family in a Wild Banana (Musa acuminata ssp. malaccensis) Uncovers Multiple Fusarium Wilt Resistance Gene Candidates
title_fullStr Genome-Wide Analysis of the LRR-RLP Gene Family in a Wild Banana (Musa acuminata ssp. malaccensis) Uncovers Multiple Fusarium Wilt Resistance Gene Candidates
title_full_unstemmed Genome-Wide Analysis of the LRR-RLP Gene Family in a Wild Banana (Musa acuminata ssp. malaccensis) Uncovers Multiple Fusarium Wilt Resistance Gene Candidates
title_short Genome-Wide Analysis of the LRR-RLP Gene Family in a Wild Banana (Musa acuminata ssp. malaccensis) Uncovers Multiple Fusarium Wilt Resistance Gene Candidates
title_sort genome-wide analysis of the lrr-rlp gene family in a wild banana (musa acuminata ssp. malaccensis) uncovers multiple fusarium wilt resistance gene candidates
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9025879/
https://www.ncbi.nlm.nih.gov/pubmed/35456444
http://dx.doi.org/10.3390/genes13040638
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