High Prevalence of Tick-Borne Zoonotic Rickettsia slovaca in Ticks from Wild Boars, Northeastern Italy

SIMPLE SUMMARY: Tick-borne rickettsioses are emerging diseases that have become widespread in many European countries, particularly in those facing the Mediterranean basin. Although Rickettsia conorii was traditionally thought to be the most threatening species, in recent decades, thanks to the improvements in biomolecular tools, other zoonotic species have been identified, such as Rickettsia slovaca, the etiological agent of scalp eschar and neck lymphadenopathy after tick bite (SENLAT), as well as other neglected species. These pathogens are present in Italy, but few data are available. This research aimed to improve the epidemiological knowledge of rickettsial infections in tick and wild boar populations in the Euganean Hills Regional Park, an enclosed area in northeastern Italy. Both tick and wild boar blood samples were tested using biomolecular methods to detect and identify Rickettsia species. Only ticks tested positive, and Rickettsia slovaca was the most frequently detected species, showing a high prevalence, followed by Rickettsia monacensis and Rickettsia helvetica. These data highlight a non-negligible presence of these pathogens in northern Italy and outline that rickettsial infections deserve further investigation. ABSTRACT: Tick-borne rickettsiae are emerging pathogens that are becoming widespread in Europe. Rickettsiae are endemic in Italy, but epidemiological data are currently scarce. This study aimed to improve our knowledge about rickettsial infections in tick and wild boar populations. Blood and ticks were collected from 102 wild boars in 2010 and 2018. Ticks were also collected from the vegetation in the area. All of the samples were examined using real-time PCR targeting the gltA gene to detect Rickettsia DNA. Positivity was confirmed by PCR amplifying the gltA and/or ompB genes. A total of 254 ticks and 89 blood samples were analyzed. Zoonotic rickettsiae were detected in the ticks but not in the blood samples. Rickettsia slovaca (R. slovaca) was the most prevalent in ticks and was found in 23.7% of Dermacentor marginatus (D. marginatus) and in 3.4% of Ixodes ricinus (I. ricinus). Other zoonotic species were identified, such as Rickettsia monacensis, which was detected in 12% of I. ricinus ticks, and Rickettsia helvetica which was found in 3.4% of questing I. ricinus ticks and in 1.1% of D. marginatus collected from wild boars. This study highlights a high prevalence of zoonotic rickettsiae, particularly that of R. slovaca, in northeastern Italy. As rickettsioses are underreported and underdiagnosed in human medicine, both clinicians and researchers should pay more attention to this topic.