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Sucrose Synthase and Fructokinase Are Required for Proper Meristematic and Vascular Development

Sucrose synthase (SuSy) and fructokinase (FRK) work together to control carbohydrate flux in sink tissues. SuSy cleaves sucrose into fructose and UDP-glucose; whereas FRK phosphorylates fructose. Previous results have shown that suppression of the SUS1,3&4 genes by SUS-RNAi alters auxin transpor...

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Autores principales: Lugassi, Nitsan, Stein, Ofer, Egbaria, Aiman, Belausov, Eduard, Zemach, Hanita, Arad, Tal, Granot, David, Carmi, Nir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9025968/
https://www.ncbi.nlm.nih.gov/pubmed/35448763
http://dx.doi.org/10.3390/plants11081035
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author Lugassi, Nitsan
Stein, Ofer
Egbaria, Aiman
Belausov, Eduard
Zemach, Hanita
Arad, Tal
Granot, David
Carmi, Nir
author_facet Lugassi, Nitsan
Stein, Ofer
Egbaria, Aiman
Belausov, Eduard
Zemach, Hanita
Arad, Tal
Granot, David
Carmi, Nir
author_sort Lugassi, Nitsan
collection PubMed
description Sucrose synthase (SuSy) and fructokinase (FRK) work together to control carbohydrate flux in sink tissues. SuSy cleaves sucrose into fructose and UDP-glucose; whereas FRK phosphorylates fructose. Previous results have shown that suppression of the SUS1,3&4 genes by SUS-RNAi alters auxin transport in the shoot apical meristems of tomato plants and affects cotyledons and leaf structure; whereas antisense suppression of FRK2 affects vascular development. To explore the joint developmental roles of SuSy and FRK, we crossed SUS-RNAi plants with FRK2-antisense plants to create double-mutant plants. The double-mutant plants exhibited novel phenotypes that were absent from the parent lines. About a third of the plants showed arrested shoot apical meristem around the transition to flowering and developed ectopic meristems. Use of the auxin reporter DR5::VENUS revealed a significantly reduced auxin response in the shoot apical meristems of the double-mutant, indicating that auxin levels were low. Altered inflorescence phyllotaxis and significant disorientation of vascular tissues were also observed. In addition, the fruits and the seeds of the double-mutant plants were very small and the seeds had very low germination rates. These results show that SUS1,3&4 and FRK2 enzymes are jointly essential for proper meristematic and vascular development, and for fruit and seed development.
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spelling pubmed-90259682022-04-23 Sucrose Synthase and Fructokinase Are Required for Proper Meristematic and Vascular Development Lugassi, Nitsan Stein, Ofer Egbaria, Aiman Belausov, Eduard Zemach, Hanita Arad, Tal Granot, David Carmi, Nir Plants (Basel) Article Sucrose synthase (SuSy) and fructokinase (FRK) work together to control carbohydrate flux in sink tissues. SuSy cleaves sucrose into fructose and UDP-glucose; whereas FRK phosphorylates fructose. Previous results have shown that suppression of the SUS1,3&4 genes by SUS-RNAi alters auxin transport in the shoot apical meristems of tomato plants and affects cotyledons and leaf structure; whereas antisense suppression of FRK2 affects vascular development. To explore the joint developmental roles of SuSy and FRK, we crossed SUS-RNAi plants with FRK2-antisense plants to create double-mutant plants. The double-mutant plants exhibited novel phenotypes that were absent from the parent lines. About a third of the plants showed arrested shoot apical meristem around the transition to flowering and developed ectopic meristems. Use of the auxin reporter DR5::VENUS revealed a significantly reduced auxin response in the shoot apical meristems of the double-mutant, indicating that auxin levels were low. Altered inflorescence phyllotaxis and significant disorientation of vascular tissues were also observed. In addition, the fruits and the seeds of the double-mutant plants were very small and the seeds had very low germination rates. These results show that SUS1,3&4 and FRK2 enzymes are jointly essential for proper meristematic and vascular development, and for fruit and seed development. MDPI 2022-04-11 /pmc/articles/PMC9025968/ /pubmed/35448763 http://dx.doi.org/10.3390/plants11081035 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lugassi, Nitsan
Stein, Ofer
Egbaria, Aiman
Belausov, Eduard
Zemach, Hanita
Arad, Tal
Granot, David
Carmi, Nir
Sucrose Synthase and Fructokinase Are Required for Proper Meristematic and Vascular Development
title Sucrose Synthase and Fructokinase Are Required for Proper Meristematic and Vascular Development
title_full Sucrose Synthase and Fructokinase Are Required for Proper Meristematic and Vascular Development
title_fullStr Sucrose Synthase and Fructokinase Are Required for Proper Meristematic and Vascular Development
title_full_unstemmed Sucrose Synthase and Fructokinase Are Required for Proper Meristematic and Vascular Development
title_short Sucrose Synthase and Fructokinase Are Required for Proper Meristematic and Vascular Development
title_sort sucrose synthase and fructokinase are required for proper meristematic and vascular development
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9025968/
https://www.ncbi.nlm.nih.gov/pubmed/35448763
http://dx.doi.org/10.3390/plants11081035
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