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Hepatitis C virus (HCV) infection among patients with sickle cell disease at the Korle-Bu teaching hospital

BACKGROUND: Hepatitis C virus (HCV) infection is a blood borne infection that remains potentially transmissible through blood transfusions. Sickle cell disease (SCD) is a common inheritable haemoglobinopathy in Ghana that requires multiple blood transfusions as part of its management. The SCD patien...

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Autores principales: Mawuli, Gifty, Dzudzor, Bartholomew, Tachi, Kenneth, Kuma, Amma Anima Benneh-Akwasi, Odame-Aboagye, James, Obeng, Billal Musah, Boateng, Anthony Twumasi, Edu-Quansah, Elijah Paa, Attiku, Keren Okyerebea, Agbosu, Esinam, Arjarquah, Augustina, Bonney, Joseph Humphrey Kofi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9026067/
https://www.ncbi.nlm.nih.gov/pubmed/35459145
http://dx.doi.org/10.1186/s12985-022-01797-z
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author Mawuli, Gifty
Dzudzor, Bartholomew
Tachi, Kenneth
Kuma, Amma Anima Benneh-Akwasi
Odame-Aboagye, James
Obeng, Billal Musah
Boateng, Anthony Twumasi
Edu-Quansah, Elijah Paa
Attiku, Keren Okyerebea
Agbosu, Esinam
Arjarquah, Augustina
Bonney, Joseph Humphrey Kofi
author_facet Mawuli, Gifty
Dzudzor, Bartholomew
Tachi, Kenneth
Kuma, Amma Anima Benneh-Akwasi
Odame-Aboagye, James
Obeng, Billal Musah
Boateng, Anthony Twumasi
Edu-Quansah, Elijah Paa
Attiku, Keren Okyerebea
Agbosu, Esinam
Arjarquah, Augustina
Bonney, Joseph Humphrey Kofi
author_sort Mawuli, Gifty
collection PubMed
description BACKGROUND: Hepatitis C virus (HCV) infection is a blood borne infection that remains potentially transmissible through blood transfusions. Sickle cell disease (SCD) is a common inheritable haemoglobinopathy in Ghana that requires multiple blood transfusions as part of its management. The SCD patient is therefore at a high risk of HCV infection; however, data on the occurrence of HCV in SCD patients has not been documented in Ghana. This study sought to determine the prevalence and genotypes of HCV infection in SCD patients. MATERIALS AND METHODS: This was a cross-sectional study which enrolled 141 sickle-cell disease patients from the Ghana Institute for Clinical Genetics, Korle-Bu Teaching Hospital (KBTH). Patient information was obtained through a structured questionnaire. Aliquots of the plasma obtained was used for both serology with Advanced Quality Rapid Anti-HCV Test Strip and molecular testing by RT-PCR with primers targeting the HCV core gene. The amplified DNA were purified and subjected to phylogenetic analysis to characterize HCV genotypes. RESULTS: Twelve (9%) out of the 141 patients were sero-positive for HCV total antibodies. HCV RNA was amplified from 8 (6%) out of the total number of patients’ samples. One of the 12 sero-positives was HCV RNA positive. Five (63%) out of the 8 HCV RNA positive samples were successfully sequenced. The phylogenetic tree constructed with the study and GenBank reference sequences, clustered all five study sequences into HCV genotype 1. CONCLUSION: The HCV seroprevalence of 9% among sickle cell disease patients is higher than reported for the general Ghanaian population which is 3%. Genotype 1 is the common HCV genotype infecting SCD patients. Sickle cell disease is likely to be a high-risk group for HCV inapparent infections in Ghana as seroprevalence does not correlate with viremia. However, even with higher seroprevalence, the group must be given priority in resource allocation for preventive, diagnostic and therapeutic strategies.
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spelling pubmed-90260672022-04-22 Hepatitis C virus (HCV) infection among patients with sickle cell disease at the Korle-Bu teaching hospital Mawuli, Gifty Dzudzor, Bartholomew Tachi, Kenneth Kuma, Amma Anima Benneh-Akwasi Odame-Aboagye, James Obeng, Billal Musah Boateng, Anthony Twumasi Edu-Quansah, Elijah Paa Attiku, Keren Okyerebea Agbosu, Esinam Arjarquah, Augustina Bonney, Joseph Humphrey Kofi Virol J Research BACKGROUND: Hepatitis C virus (HCV) infection is a blood borne infection that remains potentially transmissible through blood transfusions. Sickle cell disease (SCD) is a common inheritable haemoglobinopathy in Ghana that requires multiple blood transfusions as part of its management. The SCD patient is therefore at a high risk of HCV infection; however, data on the occurrence of HCV in SCD patients has not been documented in Ghana. This study sought to determine the prevalence and genotypes of HCV infection in SCD patients. MATERIALS AND METHODS: This was a cross-sectional study which enrolled 141 sickle-cell disease patients from the Ghana Institute for Clinical Genetics, Korle-Bu Teaching Hospital (KBTH). Patient information was obtained through a structured questionnaire. Aliquots of the plasma obtained was used for both serology with Advanced Quality Rapid Anti-HCV Test Strip and molecular testing by RT-PCR with primers targeting the HCV core gene. The amplified DNA were purified and subjected to phylogenetic analysis to characterize HCV genotypes. RESULTS: Twelve (9%) out of the 141 patients were sero-positive for HCV total antibodies. HCV RNA was amplified from 8 (6%) out of the total number of patients’ samples. One of the 12 sero-positives was HCV RNA positive. Five (63%) out of the 8 HCV RNA positive samples were successfully sequenced. The phylogenetic tree constructed with the study and GenBank reference sequences, clustered all five study sequences into HCV genotype 1. CONCLUSION: The HCV seroprevalence of 9% among sickle cell disease patients is higher than reported for the general Ghanaian population which is 3%. Genotype 1 is the common HCV genotype infecting SCD patients. Sickle cell disease is likely to be a high-risk group for HCV inapparent infections in Ghana as seroprevalence does not correlate with viremia. However, even with higher seroprevalence, the group must be given priority in resource allocation for preventive, diagnostic and therapeutic strategies. BioMed Central 2022-04-22 /pmc/articles/PMC9026067/ /pubmed/35459145 http://dx.doi.org/10.1186/s12985-022-01797-z Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visithttp://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Mawuli, Gifty
Dzudzor, Bartholomew
Tachi, Kenneth
Kuma, Amma Anima Benneh-Akwasi
Odame-Aboagye, James
Obeng, Billal Musah
Boateng, Anthony Twumasi
Edu-Quansah, Elijah Paa
Attiku, Keren Okyerebea
Agbosu, Esinam
Arjarquah, Augustina
Bonney, Joseph Humphrey Kofi
Hepatitis C virus (HCV) infection among patients with sickle cell disease at the Korle-Bu teaching hospital
title Hepatitis C virus (HCV) infection among patients with sickle cell disease at the Korle-Bu teaching hospital
title_full Hepatitis C virus (HCV) infection among patients with sickle cell disease at the Korle-Bu teaching hospital
title_fullStr Hepatitis C virus (HCV) infection among patients with sickle cell disease at the Korle-Bu teaching hospital
title_full_unstemmed Hepatitis C virus (HCV) infection among patients with sickle cell disease at the Korle-Bu teaching hospital
title_short Hepatitis C virus (HCV) infection among patients with sickle cell disease at the Korle-Bu teaching hospital
title_sort hepatitis c virus (hcv) infection among patients with sickle cell disease at the korle-bu teaching hospital
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9026067/
https://www.ncbi.nlm.nih.gov/pubmed/35459145
http://dx.doi.org/10.1186/s12985-022-01797-z
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