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QuantiFERON-TB Gold Plus Assay in Patients With Latent vs. Active Tuberculosis in a Low Incidence Setting: Level of IFN-γ, CD4/CD8 Responses, and Release of IL-2, IP-10, and MIG
OBJECTIVES: We analyzed the results of the QuantiFERON Glod Plus assay (QFT) and cytokine patterns associated with active tuberculosis (ATB) among patients with positive QFT. METHODS: A total of 195 patients are QFT-positive, among which 24 had an ATB and 171 had a latent tuberculosis infection (LTB...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9026190/ https://www.ncbi.nlm.nih.gov/pubmed/35464936 http://dx.doi.org/10.3389/fmicb.2022.825021 |
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author | Carrère-Kremer, Séverine Kolia-Diafouka, Pratt Pisoni, Amandine Bolloré, Karine Peries, Marianne Godreuil, Sylvain Bourdin, Arnaud Van de Perre, Philippe Tuaillon, Edouard |
author_facet | Carrère-Kremer, Séverine Kolia-Diafouka, Pratt Pisoni, Amandine Bolloré, Karine Peries, Marianne Godreuil, Sylvain Bourdin, Arnaud Van de Perre, Philippe Tuaillon, Edouard |
author_sort | Carrère-Kremer, Séverine |
collection | PubMed |
description | OBJECTIVES: We analyzed the results of the QuantiFERON Glod Plus assay (QFT) and cytokine patterns associated with active tuberculosis (ATB) among patients with positive QFT. METHODS: A total of 195 patients are QFT-positive, among which 24 had an ATB and 171 had a latent tuberculosis infection (LTBI). Interferon-gamma (IFN-γ) secretion was analyzed relative to interleukin-2 (IL-2), IFN-γ inducible protein or CXCL-10 (IP-10), and monokine induced by IFN-γ or CXCL-9 (MIG) secretion, and then compared between two sets of peptide antigens [tube 1 - cluster of differentiation 4 (CD4(+)) T cell stimulation; tube 2 - CD4(+)/CD8(+) T cell response]. RESULTS: Higher IFN-γ responses were measured in the ATB group (p = 0.0089). The results showed that there was a lower ratio of tube 1/tube 2 IFN-γ concentrations in the ATB group (p = 0.0009), and a median [interquartile ranges (IQR)] difference between the two sets at −0.82 IU/ml (−1.67 to 0.18) vs. −0.07 IU/ml (−0.035 to 0.11, p < 0.0001) in the ATB group compared to the LTBI group, respectively. In addition, patients with low ratios of IL-2/IFN-γ, IP-10/IFN-γ, and MIG/IFN-γ were much more likely to have ATB. CONCLUSION: High levels of IFN-γ secretion, preferential IFN-γ response in tube 2, and lower secretion of IL-2, IP-10, and MIG release relative to IFN-γ secretion were more likely observed in subjects with ATB. These features of T cell response may be helpful in low prevalence settings to suspect ATB in patients tested positive for IFN-γ release assays (IGRA). |
format | Online Article Text |
id | pubmed-9026190 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-90261902022-04-23 QuantiFERON-TB Gold Plus Assay in Patients With Latent vs. Active Tuberculosis in a Low Incidence Setting: Level of IFN-γ, CD4/CD8 Responses, and Release of IL-2, IP-10, and MIG Carrère-Kremer, Séverine Kolia-Diafouka, Pratt Pisoni, Amandine Bolloré, Karine Peries, Marianne Godreuil, Sylvain Bourdin, Arnaud Van de Perre, Philippe Tuaillon, Edouard Front Microbiol Microbiology OBJECTIVES: We analyzed the results of the QuantiFERON Glod Plus assay (QFT) and cytokine patterns associated with active tuberculosis (ATB) among patients with positive QFT. METHODS: A total of 195 patients are QFT-positive, among which 24 had an ATB and 171 had a latent tuberculosis infection (LTBI). Interferon-gamma (IFN-γ) secretion was analyzed relative to interleukin-2 (IL-2), IFN-γ inducible protein or CXCL-10 (IP-10), and monokine induced by IFN-γ or CXCL-9 (MIG) secretion, and then compared between two sets of peptide antigens [tube 1 - cluster of differentiation 4 (CD4(+)) T cell stimulation; tube 2 - CD4(+)/CD8(+) T cell response]. RESULTS: Higher IFN-γ responses were measured in the ATB group (p = 0.0089). The results showed that there was a lower ratio of tube 1/tube 2 IFN-γ concentrations in the ATB group (p = 0.0009), and a median [interquartile ranges (IQR)] difference between the two sets at −0.82 IU/ml (−1.67 to 0.18) vs. −0.07 IU/ml (−0.035 to 0.11, p < 0.0001) in the ATB group compared to the LTBI group, respectively. In addition, patients with low ratios of IL-2/IFN-γ, IP-10/IFN-γ, and MIG/IFN-γ were much more likely to have ATB. CONCLUSION: High levels of IFN-γ secretion, preferential IFN-γ response in tube 2, and lower secretion of IL-2, IP-10, and MIG release relative to IFN-γ secretion were more likely observed in subjects with ATB. These features of T cell response may be helpful in low prevalence settings to suspect ATB in patients tested positive for IFN-γ release assays (IGRA). Frontiers Media S.A. 2022-04-08 /pmc/articles/PMC9026190/ /pubmed/35464936 http://dx.doi.org/10.3389/fmicb.2022.825021 Text en Copyright © 2022 Carrère-Kremer, Kolia-Diafouka, Pisoni, Bolloré, Peries, Godreuil, Bourdin, Van de Perre and Tuaillon. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Carrère-Kremer, Séverine Kolia-Diafouka, Pratt Pisoni, Amandine Bolloré, Karine Peries, Marianne Godreuil, Sylvain Bourdin, Arnaud Van de Perre, Philippe Tuaillon, Edouard QuantiFERON-TB Gold Plus Assay in Patients With Latent vs. Active Tuberculosis in a Low Incidence Setting: Level of IFN-γ, CD4/CD8 Responses, and Release of IL-2, IP-10, and MIG |
title | QuantiFERON-TB Gold Plus Assay in Patients With Latent vs. Active Tuberculosis in a Low Incidence Setting: Level of IFN-γ, CD4/CD8 Responses, and Release of IL-2, IP-10, and MIG |
title_full | QuantiFERON-TB Gold Plus Assay in Patients With Latent vs. Active Tuberculosis in a Low Incidence Setting: Level of IFN-γ, CD4/CD8 Responses, and Release of IL-2, IP-10, and MIG |
title_fullStr | QuantiFERON-TB Gold Plus Assay in Patients With Latent vs. Active Tuberculosis in a Low Incidence Setting: Level of IFN-γ, CD4/CD8 Responses, and Release of IL-2, IP-10, and MIG |
title_full_unstemmed | QuantiFERON-TB Gold Plus Assay in Patients With Latent vs. Active Tuberculosis in a Low Incidence Setting: Level of IFN-γ, CD4/CD8 Responses, and Release of IL-2, IP-10, and MIG |
title_short | QuantiFERON-TB Gold Plus Assay in Patients With Latent vs. Active Tuberculosis in a Low Incidence Setting: Level of IFN-γ, CD4/CD8 Responses, and Release of IL-2, IP-10, and MIG |
title_sort | quantiferon-tb gold plus assay in patients with latent vs. active tuberculosis in a low incidence setting: level of ifn-γ, cd4/cd8 responses, and release of il-2, ip-10, and mig |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9026190/ https://www.ncbi.nlm.nih.gov/pubmed/35464936 http://dx.doi.org/10.3389/fmicb.2022.825021 |
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