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Metal Nanoparticle Modified Carbon-Fiber Microelectrodes Enhance Adenosine Triphosphate Surface Interactions with Fast-Scan Cyclic Voltammetry
[Image: see text] Adenosine triphosphate (ATP) is an important rapid signaling molecule involved in a host of pathologies in the body. Historically, ATP is difficult to directly detect electrochemically with fast-scan cyclic voltammetry (FSCV) due to limited interactions at bare carbon-fibers. Syste...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9026253/ https://www.ncbi.nlm.nih.gov/pubmed/35479102 http://dx.doi.org/10.1021/acsmeasuresciau.1c00026 |
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author | Li, Yuxin Keller, Alexandra L. Cryan, Michael T. Ross, Ashley E. |
author_facet | Li, Yuxin Keller, Alexandra L. Cryan, Michael T. Ross, Ashley E. |
author_sort | Li, Yuxin |
collection | PubMed |
description | [Image: see text] Adenosine triphosphate (ATP) is an important rapid signaling molecule involved in a host of pathologies in the body. Historically, ATP is difficult to directly detect electrochemically with fast-scan cyclic voltammetry (FSCV) due to limited interactions at bare carbon-fibers. Systematic investigations of how ATP interacts at electrode surfaces is necessary for developing more sensitive electrochemical detection methods. Here, we have developed gold nanoparticle (AuNP), and platinum nanoparticle (PtNP) modified carbon-fiber microelectrodes coupled to FSCV to measure the extent to which ATP interacts at metal nanoparticle-modified surfaces and to improve the sensitivity of direct electrochemical detection. AuNP and PtNPs were electrodeposited on the carbon-fiber surface by scanning from −1.2 to 1.5 V for 30 s in 0.5 mg/mL HAuCl(4) or 0.5 mg/mLK(2)PtCl(6). Overall, we demonstrate an average 4.1 ± 1.0-fold increase in oxidative ATP current at AuNP-modified and a 3.5 ± 0.3-fold increase at PtNP-modified electrodes. Metal nanoparticle-modified surfaces promoted improved electrocatalytic conversion of ATP oxidation products at the surface, facilitated enhanced adsorption strength and surface coverage, and significantly improved sensitivity. ATP was successfully detected within living murine lymph node tissue following exogenous application. Overall, this study demonstrates a detailed characterization of ATP oxidation at metal nanoparticle surfaces and a significantly improved method for direct electrochemical detection of ATP in tissue. |
format | Online Article Text |
id | pubmed-9026253 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-90262532022-04-25 Metal Nanoparticle Modified Carbon-Fiber Microelectrodes Enhance Adenosine Triphosphate Surface Interactions with Fast-Scan Cyclic Voltammetry Li, Yuxin Keller, Alexandra L. Cryan, Michael T. Ross, Ashley E. ACS Meas Sci Au [Image: see text] Adenosine triphosphate (ATP) is an important rapid signaling molecule involved in a host of pathologies in the body. Historically, ATP is difficult to directly detect electrochemically with fast-scan cyclic voltammetry (FSCV) due to limited interactions at bare carbon-fibers. Systematic investigations of how ATP interacts at electrode surfaces is necessary for developing more sensitive electrochemical detection methods. Here, we have developed gold nanoparticle (AuNP), and platinum nanoparticle (PtNP) modified carbon-fiber microelectrodes coupled to FSCV to measure the extent to which ATP interacts at metal nanoparticle-modified surfaces and to improve the sensitivity of direct electrochemical detection. AuNP and PtNPs were electrodeposited on the carbon-fiber surface by scanning from −1.2 to 1.5 V for 30 s in 0.5 mg/mL HAuCl(4) or 0.5 mg/mLK(2)PtCl(6). Overall, we demonstrate an average 4.1 ± 1.0-fold increase in oxidative ATP current at AuNP-modified and a 3.5 ± 0.3-fold increase at PtNP-modified electrodes. Metal nanoparticle-modified surfaces promoted improved electrocatalytic conversion of ATP oxidation products at the surface, facilitated enhanced adsorption strength and surface coverage, and significantly improved sensitivity. ATP was successfully detected within living murine lymph node tissue following exogenous application. Overall, this study demonstrates a detailed characterization of ATP oxidation at metal nanoparticle surfaces and a significantly improved method for direct electrochemical detection of ATP in tissue. American Chemical Society 2021-10-07 /pmc/articles/PMC9026253/ /pubmed/35479102 http://dx.doi.org/10.1021/acsmeasuresciau.1c00026 Text en © 2021 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Li, Yuxin Keller, Alexandra L. Cryan, Michael T. Ross, Ashley E. Metal Nanoparticle Modified Carbon-Fiber Microelectrodes Enhance Adenosine Triphosphate Surface Interactions with Fast-Scan Cyclic Voltammetry |
title | Metal Nanoparticle Modified Carbon-Fiber Microelectrodes
Enhance Adenosine Triphosphate Surface Interactions with Fast-Scan
Cyclic Voltammetry |
title_full | Metal Nanoparticle Modified Carbon-Fiber Microelectrodes
Enhance Adenosine Triphosphate Surface Interactions with Fast-Scan
Cyclic Voltammetry |
title_fullStr | Metal Nanoparticle Modified Carbon-Fiber Microelectrodes
Enhance Adenosine Triphosphate Surface Interactions with Fast-Scan
Cyclic Voltammetry |
title_full_unstemmed | Metal Nanoparticle Modified Carbon-Fiber Microelectrodes
Enhance Adenosine Triphosphate Surface Interactions with Fast-Scan
Cyclic Voltammetry |
title_short | Metal Nanoparticle Modified Carbon-Fiber Microelectrodes
Enhance Adenosine Triphosphate Surface Interactions with Fast-Scan
Cyclic Voltammetry |
title_sort | metal nanoparticle modified carbon-fiber microelectrodes
enhance adenosine triphosphate surface interactions with fast-scan
cyclic voltammetry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9026253/ https://www.ncbi.nlm.nih.gov/pubmed/35479102 http://dx.doi.org/10.1021/acsmeasuresciau.1c00026 |
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